Human Protein S100-A10(S100A10) ELISA kit

Instructions
Code CSB-EL020623HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name S100 calcium binding protein A10
Alternative Names 42C ELISA Kit; AA409961 ELISA Kit; AL024248 ELISA Kit; Annexin II ligand ELISA Kit; Annexin II ligand; calpactin I; light polypeptide ELISA Kit; Annexin II tetramer (AIIt) p11 subunit ELISA Kit; Annexin II; light chain ELISA Kit; ANX2L ELISA Kit; ANX2LG ELISA Kit; Ca[1] ELISA Kit; CAL12 ELISA Kit; CAL1L ELISA Kit; Calpactin I light chain ELISA Kit; Calpactin I; p11 subunit ELISA Kit; Calpactin-1 light chain ELISA Kit; Cellular ligand of annexin II ELISA Kit; CLP11 ELISA Kit; GP11 ELISA Kit; MGC111133 ELISA Kit; Nerve growth factor-induced protein 42C ELISA Kit; OTTHUMP00000015269 ELISA Kit; OTTHUMP00000015270 ELISA Kit; p10 ELISA Kit; p10 protein ELISA Kit; p11 ELISA Kit; Protein S100 A10 ELISA Kit; Protein S100-A10 ELISA Kit; S100 calcium binding protein A10 (annexin II ligand; calpactin I; light polypeptide (p11)) ELISA Kit; S100 calcium binding protein A10 (calpactin) ELISA Kit; S100 calcium binding protein A10 ELISA Kit; S100 calcium-binding protein A10 ELISA Kit; S100a10 ELISA Kit; S10AA_HUMAN ELISA Kit
Abbreviation S100A10
Uniprot No. P60903
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human S100A10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 101
Range % 97-104
1:2 Average % 108
Range % 104-112
1:4 Average % 95
Range % 91-98
1:8 Average % 89
Range % 85-94
Recovery
The recovery of human S100A10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 105 103-109
EDTA plasma (n=4) 96 93-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.044 2.125 2.085 1.936
10 1.582 1.621 1.602 1.453
5 1.146 1.154 1.150 1.001
2.5 0.679 0.731 0.705 0.556
1.25 0.466 0.451 0.459 0.310
0.625 0.352 0.362 0.357 0.208
0.312 0.235 0.222 0.229 0.080
0 0.145 0.152 0.149  
.
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.
Gene References into Functions
  1. study has localised AnxA2/S100A10 complexes to key anatomical locations in the placenta and suggests a role for this complex in amniotic epithelium, trophoblasts and syncytium, in addition to its well-known roles in endothelial cells PMID: 30143909
  2. The overexpression of ANXA2 in U937 cells transfected with full-length ANXA2 cDNA was associated with increased S100A10 subunit, although S100A10 transcripts remained constitutive. PML/RARalpha fusion protein transactivated the ANXA2 promoter to upregulate ANXA2 and accumulate S100A10. PMID: 28687976
  3. these studies define a new paradigm for plasminogen activation by the plasminogen receptor, S100A10 PMID: 28382372
  4. These findings provides evidence that gene-gene interactions between p11, tPA and BDNF are all associated with post stroke depression. PMID: 29028593
  5. Given that inflammation plays a role in both Parkinson's disease (PD)and depression, it is intriguing that peripheral p11 levels are altered in immune cells in both conditions. Our data provide insight into the pathological alterations occurring centrally and peripherally in PD. Moreover, if replicated in other cohorts, p11 could be an easily accessible biomarker PMID: 28137881
  6. The S100A10 and S100B genes, which are located on different chromosomes, encode specialized calcium-binding proteins. These data support a role for calcium homeostasis in individuals with Cannabis Dependence and high risk sex behaviours. PMID: 28418321
  7. These findings identify S100A10 as a player in endometrial receptivity acquisition. PMID: 26760977
  8. Findings indicate that Munc13-4 supports acute WPB exocytosis by tethering WPBs to the plasma membrane via AnxA2-S100A10. PMID: 28450451
  9. Here, the authors demonstrate that S100A10 is required for ULK1 localization to autophagosome formation sites. Silencing of S100A10 reduces IFN-gamma-induced autophagosome formation. PMID: 27871932
  10. p11 might be a potential regulator on 5-HTR1b and 5-HTR4 as well as a predictor of or a therapeutic target for IFN-alpha-induced depression. PMID: 26821757
  11. annexin A2 and S100A10 expressions are powerful predictors of serous ovarian cancer outcome. PMID: 26925708
  12. These data show that disruption of ANX2/p11 interaction results in reduced ALL cell adhesion to osteoblasts, increased ALL cell sensitization to chemotherapy, and suppression of ALL cell homing and engraftment. PMID: 26465153
  13. Annexin A2 complexes with S100 proteins: structure, function and pharmacological manipulation PMID: 25303710
  14. Overexpression of miR-590-5P reduced the activity of luciferase expressed by a vector bearing the 3' untranslated region of S100A10 mRNA. Ectopic miR-590-5P overexpression mediated by lentiviral infection decreased expression of S100A10. PMID: 23598417
  15. TPH1 gene polymorphisms and S100A10 expression, which correlate with 5-HT signaling were associated with ramosetron effectiveness in IBS-D, and may possibly lead to prospective identification of the resistance to treatment. PMID: 25428414
  16. Authors show here that AnxA2, p11 and AHNAK are required for type 3 secretion system-mediated Salmonella invasion of cultured epithelial cells. PMID: 23931152
  17. Data suggest a role for S100A10 as a prognostic marker and potential therapeutic target in colorectal cancer. PMID: 23828264
  18. an annexin A2-S100A10 molecular bridge participates in cell-cell interactions, revealing a hitherto unexplored function of this protein interaction PMID: 23994525
  19. complex formation of AnxA2 with S100A10 is a central regulatory mechanism in the acute release of VWF in response to cAMP-elevating agonists PMID: 23757730
  20. Suggest annexin A10 as potential marker of sessile serrated adenoma/polyps. PMID: 23595865
  21. extracellular C-1-P, acting through the extracellular annexin a2-p11 heterotetrameric protein, can mediate vascular endothelial cell invasion. PMID: 23696646
  22. Annexin A2 and S100A10 regulate human papillomavirus type 16 entry and intracellular trafficking in human keratinocytes. PMID: 23637395
  23. results demonstrate the crucial role of S100A10 in actin dynamics promoting cell spreading via Rac1 activation PMID: 23129259
  24. Binding of AHNAK to the surface of AnxA2 is governed by several hydrophobic interactions between side chains of AHNAK and pockets on S100A10. PMID: 23275167
  25. N-terminal acetylation of AnxA2 is required for S100A10 binding PMID: 23091277
  26. The AHNAK peptide adopts a coil conformation that arches across the heterotetramer contacting both annexin A2 and S100A10 protomers with tight affinity. PMID: 22940583
  27. Human chondrocytes with downregulated S100A10 showed significantly decreased production of inflammatory cytokines such as tumor necrosis factor-alpha, IL-1beta and IL-10; hence, S100A10 might be considered a potential target for anti-inflammatory treatment PMID: 22797859
  28. Annexin A2 anchors S100A10 to the cell surface and, in doing so, allows S100A10 to play a prominent role in the activation of plasminogen in angiogenesis and oncogenesis. PMID: 22830395
  29. annexin A2 heterotetramer contributes to HPV16 internalization and infection of epithelial cells and this interaction is dependent on the presence of the L2 minor capsid protein PMID: 22927980
  30. COX7A2, TAGLN2 and S100-A10 as novel prognostic markers in Barrett's adenocarcinoma. PMID: 22365974
  31. the overexpression of thioredoxin,S100-A10 and S100-A6 specifically distinguished metastatic from non-metastatic tumors. PMID: 21938494
  32. Interferon-gamma stimulates p11-dependent surface expression of annexin A2 in lung epithelial cells to enhance phagocytosis. PMID: 21928315
  33. This study demonstrates that PBMC p11 mRNA expression is associated with neural activation in the brain of BD patients and warrants a larger translational study to determine its clinical utility. PMID: 21722919
  34. Both the annexin A2 and p11 subunits of calpactin I coimmunoprecipitate with human papillomavirus type 16 E5 in COS cells and in human epithelial cell lines, and an intact E5 C terminus is required for binding. PMID: 21849434
  35. Understanding the chromatin remodeling involved in the glucocorticoid-mediated increase of p11 expression by stress may clarify stress-induced over-expression of p PMID: 21367534
  36. The results of this study suggested that PBMC p11 mRNA levels may be a potential adjunctive biomarker for the assessment of suicide risk in mental disorders and warrants a larger translational study to determine its clinical utility. PMID: 20863517
  37. DLC1 binding to S100A10 did not affect DLC1's RhoGAP activity, but it decreased the steady-state level of S100A10 expression. PMID: 21372205
  38. S100A10 plays a crucial role in the generation of plasmin leading to fibrinolysis, thus providing a link to the clinical hemorrhagic phenotype of acute promyelocytic leukemia PMID: 21310922
  39. The present study represents a first attempt to systematically understand the molecular basis for the calcium-insensitive open conformation of S100A10. PMID: 21269277
  40. CFTR function by annexin A2-S100A10 complex has roles in health and disease [review] PMID: 20093721
  41. First insights of S100A10 function as a regulator of the filamentous actin network. PMID: 20100475
  42. These results suggest that epidermal growth factor treatment increased p11 bound to cPLA(2) may lead to the late suppression of AA release induced by EGF. PMID: 12163506
  43. p11 interacts specifically with the TASK-1 K+ channel. PMID: 12198146
  44. Calpactin light chain binds to bluetongue virus NS3 protein PMID: 12235365
  45. IFN-gamma-stimulated p11 expression may serve a counterregulatory role in human epithelial cells PMID: 12645529
  46. analysis of S100A10 interaction with tissue plasminogen activator, plasminogen, and plasmin PMID: 12730231
  47. annexin 2/S100A10 complex functions in the intracellular positioning of recycling endosomes and that both subunits are required for this activity PMID: 13679511
  48. Temperature stress-induced annexin 2 translocation is dependent on both expression of protein p11 tyrosine phosphorylation of annexin 2 PMID: 15302870
  49. S100A10 and annexin A2 play an important role in plasmin regulation and in cancer cell invasiveness and metastasis [review] PMID: 15574370
  50. complex with annexin II is a substrate of thioredoxin PMID: 15849182

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Protein Families S-100 family
Database Links

HGNC: 10487

OMIM: 114085

KEGG: hsa:6281

STRING: 9606.ENSP00000357799

UniGene: Hs.143873

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