Human Protein Wnt-16(WNT16) ELISA kit

Code CSB-EL026132HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name wingless-type MMTV integration site family, member 16
Alternative Names Protein Wnt 16 precursor ELISA Kit; Protein Wnt-16 ELISA Kit; Wingless type MMTV integration site family member 16 ELISA Kit; WNT16 ELISA Kit; WNT16_HUMAN ELISA Kit
Abbreviation WNT16
Uniprot No. Q9UBV4
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Stem Cells
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human WNT16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %87
Range %82-93
1:2Average %102
Range %97-107
1:4Average %98
Range %95-102
1:8Average %93
Range %90-96
Recovery
The recovery of human WNT16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8581-92
EDTA plasma (n=4)10097-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.452 2.487 2.470 2.293
10001.716 1.736 1.726 1.549
5001.111 1.149 1.130 0.953
2500.673 0.698 0.686 0.509
1250.465 0.468 0.467 0.290
62.50.341 0.362 0.352 0.175
31.250.287 0.276 0.282 0.105
00.176 0.178 0.177
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Gene References into Functions
  1. there was a significant difference in the genotypic frequencies of rs2707466 between hypertrophic and atrophic hip OA in males, with overrepresentation of G alleles in the hypertrophic phenotype. An association in the same direction was observed between these alleles and the type of knee OA, with G alleles being more common in the hypertrophic than in atrophic knee phenotypes PMID: 28766055
  2. WNT16B recognizes cancer cell surface receptors including frizzled (FZD) 3/4/6, a process enhanced by SFRP2, coordinated by the co-receptor LRP6 but subject to abrogation by DKK1. PMID: 26751775
  3. PRKX, WNT3 and WNT16 genes, belonging to the WNT signaling pathway, are involved in the tumorigenic process of nodular basal cell carcinoma PMID: 27630294
  4. ThE findingS suggests that WNT16 might be an important genetic factor in determining peak bone mass acquisition. PMID: 26510844
  5. Although in vitro studies demonstrated no differences in expressions of wild-type and mutant forms of IDUA and WNT16B proteins, in silico analyses predicts that WNT16 rs2707466 directly abolishes a phosphorylation site, which could cause a deleterious effect on WNT16 protein. PMID: 26256109
  6. MicroRNA-374b Suppresses Proliferation and Promotes Apoptosis in T-cell Lymphoblastic Lymphoma by Repressing AKT1 and Wnt-16 PMID: 26100275
  7. Data indicate that WNT16 is critical for positive regulation of both cortical and trabecular bone mass and structure. WNT16-TG mice exhibited significantly higher whole-body areal bone mineral density and bone mineral content. PMID: 26584014
  8. ALL cells expressing WNT16 are sensitive to endoplasmic reticulum stress, and show enhanced killing after addition of chloroquine. PMID: 26254351
  9. loss of endogenous WNT16 results specifically in cortical bone loss, whereas overexpression of WNT16 surprisingly increases mainly trabecular bone mass. PMID: 26627248
  10. Describe TGFbeta-Wnt16-Notch signaling conduit in the chondrocyte-like transformation of VSMCs and identify endogenous TGFbeta activity in MGP-null VSMCs as a critical mediator of chondrogenesis. PMID: 25614285
  11. variants at WNT16 were more strongly related to upper limb-bone mineral density, than to bone mineral density at the other sites. PMID: 24945404
  12. Osteoblast-derived WNT16 is a previously unreported key regulator of osteoclastogenesis and fracture susceptibility. PMID: 25306233
  13. Increased translation of WNT16 can thus lead to an increased inhibitory action of WNT16 on canonical WNT signaling. PMID: 24185276
  14. Common missense polymorphisms of the WNT16 gene are associated with bone mineral density at the hip, calcaneal ultrasound and the buckling ratio of the femoral neck, as well as with hip fractures in individuals under 80 years of age. PMID: 23417354
  15. Estrogen/progesterone treatment of mature myometrial cells induced expression of WNT11 and WNT16, which remained constitutively elevated in leiomyoma tissues. PMID: 24082114
  16. results suggest synergistic effects of WNT16a insertion and the at-risk 'T' allele of TCF7L2 (rs7903146) for elevating the expression of TCF7L2 in human pancreas which may affect the regulation of downstream target genes involved in the development of T2D PMID: 23617586
  17. Analyses of femoral neck BMD also supported association with SNPs in WNT16 and ESR1/C6orf97 PMID: 23074152
  18. Treatment-induced damage to the tumor microenvironment promotes prostate cancer therapy resistance through WNT16B. PMID: 22863786
  19. these findings postulate that the WNT16/C7orf58 locus contains complex patterns of genetic variation, which play an important role in peak bone mass accrual and may likely impact BMD determination at later life. PMID: 22792070
  20. These results suggest a critical role of Wnt 16 signaling pathway on cortical bone thickness and bone strength determination as well as fracture susceptibility. PMID: 22792071
  21. Results demonstrated significant up-regulation of WNT-3, WNT-4, WNT-5B, WNT-7B, WNT-9A, WNT-10A, and WNT-16B in patients with CLL compared to normal subjects. PMID: 19863181
  22. study identified WNT16B as a new marker of senescence that regulates p53 activity and the PI3K/AKT pathway and is necessary for the onset of replicative senescence PMID: 19951988
  23. genomic analysis of conserved sequences between human, rat, and zebrafish WNT16 PMID: 15756456
  24. Targeted-Wnt16b inhibition leads to apoptotic cell death, and Wnt16b mediates its effect through canonical Wnt pathway involving dishevelled-2, beta-catenin and survivin; Wnt16 plays an important role in leukemogenesis. PMID: 16007226
  25. Targeted-E2A-PBX1 inhibition leads to reduced expression of the EB-1 and Wnt16b genes; aberrant expression of these genes may be a key step in leukemogenesis in t(1;19)-positive pre-B leukemia. PMID: 16769578
  26. Wnt16 does not activate canonical Wnt signaling in E2A-PBX1-positive cells. Instead, beta-catenin is involved in N-cadherin-dependent adherence junctions PMID: 19101069

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Subcellular Location Secreted, extracellular space, extracellular matrix.
Protein Families Wnt family
Tissue Specificity Isoform Wnt-16b is expressed in peripheral lymphoid organs such as spleen, appendix, and lymph nodes, in kidney but not in bone marrow. Isoform Wnt-16a is expressed at significant levels only in the pancreas.
Database Links

HGNC: 16267

OMIM: 606267

KEGG: hsa:51384

STRING: 9606.ENSP00000222462

UniGene: Hs.272375

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