Human Protein notum homolog(NOTUM) ELISA kit

Code CSB-EL015955HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
notum pectinacetylesterase homolog (Drosophila)
Alternative Names
NOTUM ELISA Kit; notum pectinacetylesterase homolog (Drosophila) ELISA Kit; Notum pectinacetylesterase homolog ELISA Kit; NOTUM_HUMAN ELISA Kit; Protein notum homolog ELISA Kit
Uniprot No.
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 ng/mL-100 ng/mL
0.39 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human NOTUM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 90-100  
1:2 Average % 89  
Range % 85-93  
1:4 Average % 92  
Range % 88-96  
1:8 Average % 85  
Range % 80-90  
The recovery of human NOTUM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-100  
EDTA plasma (n=4) 106 100-114  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
100 2.607 2.497 2.552 2.378  
50 2.203 2.075 2.139 1.965  
25 1.635 1.542 1.589 1.415  
12.5 0.952 1.006 0.979 0.805  
6.25 0.610 0.587 0.599 0.425  
3.12 0.451 0.462 0.457 0.283  
1.56 0.332 0.315 0.324 0.150  
0 0.176 0.171 0.174    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Human NOTUM ELISA Kit was designed for the quantitative measurement of Human NOTUM protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

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Target Background

(From Uniprot)
Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.
Gene References into Functions
  1. NOTUM expression was increased in metastatic cells. Proliferation was suppressed by inhibiting expression of NOTUM. Knockdown of NOTUM genes inhibited proliferation as well as migration, with possible involvement of p38 and c-JUN N-terminal kinase in this process. PMID: 30343282
  2. Data show that notum and glypican-1 and glypican-3 gene expression during colorectal cancer (CRC) development and present evidence to suggest them as potential new biomarkers of CRC pathogenesis. PMID: 26517809
  3. Kinetic and mass spectrometric analyses of human proteins show that Notum is a carboxylesterase that removes an essential palmitoleate moiety from Wnt proteins and thus constitutes the first known extracellular protein deacylase PMID: 25731175
  4. Overexpression of NOTUM is associated with hepatocellular carcinoma PMID: 18429952
Subcellular Location
Protein Families
Pectinacetylesterase family, Notum subfamily
Tissue Specificity
Rarely expressed in adult normal tissues.
Database Links

HGNC: 27106

OMIM: 609847

KEGG: hsa:147111

STRING: 9606.ENSP00000387310

UniGene: Hs.106137

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