Human Putative humanin peptide(MT-RNR2) ELISA kit

Instructions
Code CSB-EL015084HU
Size 96T,5×96T,10×96T

Product Details

Target Name mitochondrially encoded 16S RNA
Alternative Names MTRNR2,
Abbreviation MT-RNR2
Uniprot No. Q8IVG9
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 28 pg/mL-1800 pg/mL
Sensitivity 7 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MT-RNR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 100  
Range % 92-105  
1:10 Average % 97  
Range % 91-105  
1:20 Average % 97  
Range % 92-106  
1:40 Average % 93  
Range % 86-98  
Recovery
The recovery of human MT-RNR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 85-99  
EDTA plasma (n=4) 95 90-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1800 2.120 2.163 2.142 2.007  
900 1.675 1.605 1.640 1.505  
450 1.158 1.168 1.163 1.028  
225 0.741 0.780 0.761 0.626  
112.5 0.352 0.383 0.368 0.233  
56.25 0.231 0.241 0.236 0.101  
28 0.178 0.186 0.182 0.047  
0 0.132 0.138 0.135    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Plays a role as a neuroprotective factor. Protects against death induced by multiple different familial Alzheimer disease genes and amyloid-beta proteins in Alzheimer disease. Suppresses apoptosis by binding to BAX and preventing the translocation of BAX from the cytosol to mitochondria. Binds to IGFBP3 and specifically blocks IGFBP3-induced cell death Induces chemotaxis of mononuclear phagocytes via FPR2. Reduces the aggregation and fibrillary formation by suppressing the effect of APP on mononuclear phagocytes and acts by competitively inhibiting the access of FPRL1 to APP.
Subcellular Location Secreted, Cytoplasm
Protein Families Humanin family
Tissue Specificity Expressed in the heart, skeletal muscles, kidney and liver. Lesser but significant expression is observed in the brain and the gastrointestinal tract. Expressed in the AD brain, where it is found in some of the large intact neurons of the occipital lobes
Database Links

HGNC: 7471

OMIM: 606120

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