Human Repetin(RPTN) ELISA kit

Code CSB-EL020501HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
repetin
Alternative Names
RPTN ELISA Kit; Repetin ELISA Kit
Abbreviation
RPTN
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.625 ng/mL-40 ng/mL
Sensitivity
0.156 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human RPTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 88-97  
1:2 Average % 86  
Range % 80-92  
1:4 Average % 96  
Range % 87-102  
1:8 Average % 93  
Range % 89-97  
Recovery
The recovery of human RPTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 88-98  
EDTA plasma (n=4) 90 87-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
40 2.796 2.869 2.833 2.711  
20 2.131 1.994 2.063 1.941  
10 1.152 1.243 1.198 1.076  
5 0.705 0.732 0.719 0.597  
2.5 0.343 0.366 0.355 0.233  
1.25 0.236 0.224 0.230 0.108  
0.625 0.218 0.210 0.214 0.092  
0 0.124 0.120 0.122    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human RPTN ELISA Kit was designed for the quantitative measurement of Human RPTN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .

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Target Background

Function
(From Uniprot)
Involved in the cornified cell envelope formation. Multifunctional epidermal matrix protein. Reversibly binds calcium.
Gene References into Functions
  1. The single-nucleotide polymorphisms of CRNN (rs941934) and RPTN (rs3001978, rs28441202) may contribute to Atopic Dermatitis development, but further studies on a larger group of Atopic Dermatitis patients are needed to verify this assumption. PMID: 28219068
  2. RPTN plays a potential role in emotional and cognitive processing; its decrease in serum may indicate its involvement in the pathogenesis of schizophrenia and bipolar disorder PMID: 25613293
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
S100-fused protein family
Tissue Specificity
Expression is scattered in the normal epidermis but strong in the acrosyringium, the inner hair root sheath and in the filiform papilli of the tongue.
Database Links

HGNC: 26809

OMIM: 613259

KEGG: hsa:126638

STRING: 9606.ENSP00000317895

UniGene: Hs.376144

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