Human Reticulon-4(RTN4) ELISA kit

Instructions
Code CSB-EL020572HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name reticulon 4
Alternative Names ASY, NI220/250, NOGO, NOGO-A, NOGOC, NSP, NSP-CL, Nbla00271, Nbla10545, Nogo-B, Nogo-C, RTN-X, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C, Human NogoA|My043 protein|OTTHUMP00000201714|OTTHUMP00000201801|OTTHU
Abbreviation RTN4
Uniprot No. Q9NQC3
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 46.88 pg/mL-3000 pg/mL
Sensitivity 11.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human RTN4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 97    
Range % 92-101    
1:2 Average % 92    
Range % 88-96    
1:4 Average % 89    
Range % 84-93    
1:8 Average % 96    
Range % 91-100    
Recovery
The recovery of human RTN4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 103 99-108    
EDTA plasma (n=4) 84 80-88    
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
3000 2.664 2.728 2.696 2.502    
1500 2.120 1.994 2.057 1.863    
750 1.200 1.251 1.226 1.032    
375 0.753 0.785 0.769 0.575    
187.5 0.439 0.462 0.451 0.257    
93.75 0.315 0.307 0.311 0.117    
46.88 0.247 0.239 0.243 0.049    
0 0.191 0.196 0.194      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. Induces the formation and stabilization of endoplasmic reticulum (ER) tubules
Subcellular Location Endoplasmic reticulum membrane, Multi-pass membrane protein
Tissue Specificity Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis-specific.
Database Links

HGNC: 14085

OMIM: 604475

KEGG: hsa:57142

STRING: 9606.ENSP00000337838

UniGene: Hs.637850

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