Human Ribonuclease 7(RNASE7) ELISA kit

Code CSB-EL019798HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
ribonuclease, RNase A family, 7
Alternative Names
EC 3.1.27.- ELISA Kit; MGC133220 ELISA Kit; Ribonuclease 7 ELISA Kit; ribonuclease, RNase A family, 7 ELISA Kit; RNAS7_HUMAN ELISA Kit; RNase 7 ELISA Kit; RNASE7 ELISA Kit; SAP 2 ELISA Kit; SAP-2 ELISA Kit; Skin derived antimicrobial protein 2 ELISA Kit; Skin-derived antimicrobial protein 2 ELISA Kit
Abbreviation
RNASE7
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, urine, saliva, cell culture supernates, tissue homogenates, cell lysates
Detection Range
1.56 ng/mL-100 ng/mL
Sensitivity
0.39 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Epigenetics and Nuclear Signaling
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human RNASE7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 100  
Range % 95-105  
1:2 Average % 98  
Range % 93-103  
1:4 Average % 89  
Range % 80-95  
1:8 Average % 88  
Range % 82-99  
Recovery
The recovery of human RNASE7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 100 95-105  
EDTA plasma (n=4) 104 100-109  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
100 2.410 2.456 2.433 2.256  
50 1.618 1.629 1.624 1.447  
25 1.076 1.098 1.087 0.910  
12.5 0.726 0.703 0.715 0.538  
6.25 0.490 0.483 0.487 0.310  
3.12 0.384 0.354 0.369 0.192  
1.56 0.269 0.282 0.276 0.099  
0 0.178 0.176 0.177    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human RNASE7 ELISA Kit was designed for the quantitative measurement of Human RNASE7 protein in serum, plasma, urine, saliva, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL .

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Target Background

Function
(From Uniprot)
Exhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium. Causes loss of bacterial membrane integrity. Probably contributes to urinary tract sterility. Bactericidal activity is independent of RNase activity.
Gene References into Functions
  1. These data suggest that the induction of RNase 7 in response to Mycobacterium tuberculosis could have a role in anti-mycobacterial immunity. PMID: 29346642
  2. RNase 7 has immunomodulatory functions on TH2 cells and decreases the production of TH2 cytokines in the skin. PMID: 28378334
  3. Our results indicate that RNase 7 is an important factor released by keratinocytes to control the growth of P. aeruginosa on the skin surface. PMID: 27513608
  4. Insulin and PI3K/AKT signaling are essential for RNase 7 expression and increased infection risks in diabetic patients may be secondary to suppressed RNase 7 production. PMID: 27401534
  5. This article provides an overview about the role of RNase 7 in cutaneous defense with focus on the molecular mechanism of the antimicrobial activity of RNase 7, the regulation of RNase 7 expression, and the role of RNase 7 in skin diseases. [review] PMID: 27089327
  6. RNH1 bound to RNase 7 and suppressed its antimicrobial activity by blocking its ability to bind the cell wall of uropathogenic bacteria. PMID: 24107847
  7. RNase 7 is an antimicrobial protein that plays an important role in the innate immune defense system of human epithelia. PMID: 12244054
  8. Suggest that RNase 7 may function as antimicrobial peptide with a role in the differentiation and development of the primitive epidermis. PMID: 23545750
  9. the influence of STAT3 on the IL-17A/IFN-gamma -mediated RNase 7 induction PMID: 23555696
  10. RNase 7 expression is increased in the urinary tract with infection and has antibacterial activity against uropathogens at micromolar concentrations. PMID: 23302724
  11. a complex relationship between tear induction of miR-762, its modulation of innate defense genes, and P. aeruginosa internalization PMID: 23469087
  12. RNASE7 prevents staphylococcal skin infections PMID: 20668470
  13. proteases contribute indirectly to the defense function of the stratum corneum by releasing the RNase inhibitor mediated inhibition of RNase 5 and RNase 7. PMID: 19262607
  14. Using phospholipid vesicles as membrane models, RNase 3 triggers first the vesicle aggregation, while RNase 7 induces leakage well before the aggregation step. PMID: 19366593
  15. data indicate that RNase 7 contributes to the E. faecium-killing activity of skin extracts and suggest an important role for RNase 7 in the protection of human skin against E. faecium colonization PMID: 19641608
  16. These findings suggest that antimicrobial peptides found at high baseline levels in healthy skin, such as RNase 7, confer protection against Staphylococcus aureus infection of the skin. PMID: 19919305

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Subcellular Location
Secreted.
Protein Families
Pancreatic ribonuclease family
Tissue Specificity
Expressed in collecting ducts in kidney, and in apical uroepithelium in bladder (at protein level). Expressed in various epithelial tissues including skin, respiratory tract, genito-urinary tract and, at a low level, in the gut. Expressed in liver, kidney
Database Links

HGNC: 19278

OMIM: 612484

KEGG: hsa:84659

STRING: 9606.ENSP00000298690

UniGene: Hs.525206

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