Human SPARC-related modular calcium-binding protein 2(SMOC2) ELISA kit

Instructions
Code CSB-EL021843HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name SPARC related modular calcium binding 2
Alternative Names bA270C4A.1 ELISA Kit; bA37D8.1 (secreted modular calcium binding protein 2) ELISA Kit; bA37D8.1 ELISA Kit; dJ421D16.1 (novel thyroglobulin type 1 repeat containing protein) ELISA Kit; dJ421D16.1 ELISA Kit; MST117 ELISA Kit; MSTP117 ELISA Kit; MSTP140 ELISA Kit; Secreted modular calcium binding protein 2 ELISA Kit; Secreted modular calcium-binding protein 2 ELISA Kit; SMAP 2 ELISA Kit; SMAP-2 ELISA Kit; SMAP2 ELISA Kit; SMOC 2 ELISA Kit; SMOC-2 ELISA Kit; SMOC2 ELISA Kit; SMOC2_HUMAN ELISA Kit; Smooth muscle associated protein 2 ELISA Kit; Smooth muscle-associated protein 2 ELISA Kit; SPARC related modular calcium binding 2 ELISA Kit; SPARC-related modular calcium-binding protein 2 ELISA Kit
Abbreviation SMOC2
Uniprot No. Q9H3U7
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SMOC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %92-103
1:2Average %100
Range %96-104
1:4Average %93
Range %87-97
1:8Average %99
Range %92-103
Recovery
The recovery of human SMOC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9187-95
EDTA plasma (n=4)9488-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.272 2.211 2.242 2.094
51.794 1.765 1.780 1.632
2.51.190 1.123 1.157 1.009
1.250.787 0.754 0.771 0.623
0.6250.493 0.482 0.488 0.340
0.3120.336 0.348 0.342 0.194
0.1560.236 0.221 0.229 0.081
00.149 0.147 0.148
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Promotes matrix assembly and cell adhesiveness (By similarity). Can stimulate endothelial cell proliferation, migration, as well as angiogenesis.
Gene References into Functions
  1. SMOC2 was highly expressed in both peritoneal and endometrioma lesions. Considering that the genes studied participate either directly or indirectly in cellular processes that can lead to cell migration, angiogenesis, and inappropriate invasion, it is possible that the deregulation of these genes caused the development and maintenance of ectopic tissue. PMID: 28678915
  2. The expression of Smoc2 was significantly higher in hepatocellular carcinoma (HCC) tissues compared with corresponding non-tumor liver tissues. Smoc2 promotes the proliferation of HCC cells through accelerating cell cycle progression. PMID: 28018113
  3. L1-mediated CRC progression involves the acquisition of a stem cell-like phenotype, and that SMOC-2 elevation is necessary for L1-mediated induction of more aggressive/invasive CRC properties. PMID: 25915847
  4. could not detect an association between SMOC2 gene and AMD. This study tests the candidacy of SMOC2 gene to the etiology of AMD PMID: 22964816
  5. SMOC2 SNP may play a role in autoimmune thyroid disease susceptibility as a dominant polymorphism. PMID: 23463390
  6. mutation in exon 8 of the SMOC2 gene linked to oligodontia PMID: 23317772
  7. Homozygosity mapping and candidate prioritization identify mutations, missed by whole-exome sequencing, in SMOC2, causing major dental developmental defects PMID: 22152679
  8. The variant rs13208776 in SMOC2 gene does not play a major role in increasing the risk of vitiligo in Jordanian Arab patients. PMID: 20965805
  9. SMOC2 is a risk locus for generalized vitiligo and perhaps other autoimmune diseases PMID: 19890347
  10. identification of gene, chromosome mapping PMID: 12741954
  11. SMOC-2 is a novel angiogenic factor that potentiates angiogenic effects of growth factors PMID: 16774925
  12. We tested association between SMOC2 polymorphisms and FEV(1) and FVC. SMOC2 might play an important role in the determination of FEV(1) and FVC PMID: 17204727
  13. Although this study specifies rat, the authors studied and discussed the human gene in this study as well. PMID: 12031507

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Involvement in disease Dentin dysplasia 1 (DTDP1)
Subcellular Location Secreted, extracellular space, extracellular matrix, basement membrane
Database Links

HGNC: 20323

OMIM: 125400

KEGG: hsa:64094

STRING: 9606.ENSP00000346537

UniGene: Hs.487200

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