Human Secretoglobin family 3A member 2(SCGB3A2) ELISA kit

Product Details

Citations

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Customer Reviews and Q&A

Target Background

Product Details

Target Name

secretoglobin, family 3A, member 2

Alternative Names

LU103 ELISA Kit; Pneumo secretory protein 1 ELISA Kit; PnSP-1 ELISA Kit; PNSP1 ELISA Kit; SCGB3A2 ELISA Kit; Secretoglobin family 3A member 2 ELISA Kit; SG3A2_HUMAN ELISA Kit; UGRP1 ELISA Kit; Uteroglobin-related protein 1 ELISA Kit

Abbreviation

SCGB3A2

Uniprot No.

Q96PL1

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

46.88 pg/mL-3000 pg/mL

Sensitivity

11.72 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Epigenetics and Nuclear Signaling

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SCGB3A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-98
1:2	Average %	88
	Range %	81-92
1:4	Average %	104
	Range %	98-111
1:8	Average %	97
	Range %	83-93

Recovery

The recovery of human SCGB3A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-99
EDTA plasma (n=4)	95	89-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.703	2.752	2.728	2.606
1500	1.906	1.866	1.886	1.764
750	1.128	1.200	1.164	1.042
375	0.545	0.539	0.542	0.420
187.5	0.316	0.332	0.324	0.202
93.75	0.205	0.208	0.207	0.085
46.88	0.186	0.200	0.193	0.071
0	0.121	0.122	0.122

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human SCGB3A2 ELISA Kit was designed for the quantitative measurement of Human SCGB3A2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 46.88 pg/mL-3000 pg/mL and the sensitivity is 11.72 pg/mL .

Target Background

Function
(From Uniprot)

Secreted cytokine-like protein. Binds to the scavenger receptor MARCO. Can also bind to pathogens including the Gram-positive bacterium L.monocytogenes, the Gram-negative bacterium P.aeruginosa, and yeast. Strongly inhibits phospholipase A2 (PLA2G1B) activity. Seems to have anti-inflammatory effects in respiratory epithelium. Also has anti-fibrotic activity in lung. May play a role in fetal lung development and maturation. Promotes branching morphogenesis during early stages of lung development. In the pituitary, may inhibit production of follicle-stimulating hormone (FSH) and luteinizing hormone (LH).

Gene References into Functions

rs151333009 SNP showed a monomorphic genotype. Two promoter SNPs (rs6882292, -659 G/A and rs1368408, -112 G/A) showed significant association with asthma. These results suggest that the promoter SNPs (rs6882292 and rs1368408) of SCGB3A2 gene may contribute to susceptibility to asthma in a Korean population. PMID: 28422086
This meta-analysis suggests that SCGB3A2 polymorphism at positions -112G>A was associated with Graves' disease both in Chinese and Caucasian population. PMID: 23934357
the SCGB3A2 -112G >A polymorphism may be considered as a likely marker linking susceptibility to allergy/asthma and Graves' disease PMID: 21170691
Reduced production of UGRP1, which is likely due, at least in part, to a local cytokine environment, may contribute to the hyper-inflammation in chronic rhinosinusitis and correlates with response to surgery. PMID: 21385388
This study documents the association between polymorphisms in UGRP1 and the common cold, suggesting a potential role in its pathogenesis. PMID: 21410962
Results demonstrate that SCGB3A2 is a useful marker for diagnosis of pulmonary tumors both in mice and humans. PMID: 20466451
pattern of UGRP-1 concentration resembled that of Clara cell protein, both proteins occurring in high concentrations in amniotic fluid, sputum and BALF and in much lower concentrations in serum and urine PMID: 20843168
association was detected between SCGB3A2 and U.K. Caucasian Graves' disease subjects but the size of effect was smaller than that seen in the Oriental population (odds ratio = 1.28-1.73). PMID: 20210668
polymorphism in the human UGRP1 gene promoter that regulates transcription is associated with an increased risk of asthma PMID: 11813133
By in situ hybridization, UGRP1 is found to be expressed by lung Clara-like cells in the bronchial epithelium and up-regulated in lung epithelium of a patient with cystic fibrosis. PMID: 12847263
results demonstrate that interleukin-10 induces uteroglobin related protein 1(UGRP1) gene expression in lung epithelial cells through transcription factor T/EBP/NKX2.1-dependent pathway PMID: 15485815
plasma UGRP1 levels were associated with the G-112A UGRP1 gene promoter polymorphism and the severity of asthma PMID: 18089940
results suggest that the -112G/A polymorphism does not play a significant role in the genetic predisposition of the UGRP1 gene in atopic asthma in the Sicilian population PMID: 18201431
SCGB3A2 gene may contribute to Graves' disease susceptibility. PMID: 19126779

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Subcellular Location

Secreted.

Protein Families

Secretoglobin family, UGRP subfamily

Tissue Specificity

Highly expressed in lung and trachea. Detected throughout the airway epithelium in lung, with slightly higher expression in large airways. Found in lung submucosal gland acinus where it localizes to serous-like cells. Probably expressed in club/Clara cell

Database Links

HGNC: 18391

OMIM: 606531

KEGG: hsa:117156

STRING: 9606.ENSP00000296694

UniGene: Hs.483765

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Code	CSB-EL020819HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.