Human Semaphorin-4A(SEMA4A) ELISA kit

Product Details

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Customer Reviews and Q&A

Target Background

Name: Human Semaphorin-4A(SEMA4A) ELISA kit
Brand: CUSABIO
SKU: CSB-EL020987HU

Product Details

Target Name

sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A

Alternative Names

CORD10 ELISA Kit; RP11 54H19 2 ELISA Kit; RP35 ELISA Kit; SEM4A_HUMAN ELISA Kit; Sema B ELISA Kit; Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain 4A ELISA Kit; Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4A ELISA Kit; Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) ELISA Kit; Sema4a ELISA Kit; SEMAB ELISA Kit; Semaphorin 4A precursor ELISA Kit; Semaphorin B ELISA Kit; Semaphorin-4A ELISA Kit; Semaphorin-B ELISA Kit; SEMB ELISA Kit

Abbreviation

SEMA4A

Uniprot No.

Q9H3S1

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SEMA4A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-93
1:2	Average %	97
	Range %	93-100
1:4	Average %	91
	Range %	88-95
1:8	Average %	94
	Range %	91-99

Recovery

The recovery of human SEMA4A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	83	80-86
EDTA plasma (n=4)	87	81-90

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.604	2.635	2.620	2.478
10	1.863	1.899	1.881	1.739
5	1.021	1.036	1.029	0.887
2.5	0.564	0.532	0.548	0.406
1.25	0.315	0.335	0.325	0.183
0.625	0.259	0.271	0.265	0.123
0.312	0.206	0.199	0.203	0.061
0	0.141	0.143	0.142

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human SEMA4A ELISA Kit was designed for the quantitative measurement of Human SEMA4A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Customer Reviews and Q&A

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■ Q&A

1) Can you give me any more information on the standards used in the kit? i.e. if they are full length proteins, are they recombinant, what kind of expression systems have they been made in? Thanks
2) I would also be interested to know what kind of antibodies are used for the capture and detection - i.e. the species of antibody, whether they are polyclonal or monoclonal, which immunogen they were raised against and also whether they are available to purchase separately?

Thanks for your inquiry! Pls check our information:
Standards:293 cell-derived recombinant protein
Coated anti: Mouse monoclonal antibody
Detect anti: Goat polyclonal antibody
Immunogen:293 cell-derived recombinant protein
The detailed sequence of standard and immunogen is our confidential information and cannot be provided.
All the components are the set of our kit and cannot be provided separately.

A customer interested in quantifying human semaphorin-4A from serum samples. They are particularly interested in quantifying the extracellular portion of such protein since not all my samples have the cytoplasmic region.

Thanks for your inquiry!
CSB-EL020987HU
This kit can test serum samples.This kit is designed against extracellular domain. Pls refer to: https://www.uniprot.org/uniprot/Q9H3S1 .
Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)

Cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling. Regulates glutamatergic and GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner and promotes the development of excitatory synapses in a PLXNB2-dependent manner. Plays a role in priming antigen-specific T-cells, promotes differentiation of Th1 T-helper cells, and thereby contributes to adaptive immunity. Promotes phosphorylation of TIMD2. Inhibits angiogenesis. Promotes axon growth cone collapse. Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.

Gene References into Functions

Human oligodendrocytes undergo apoptosis when exposed to Sema4A and take up H-ferritin for meeting iron requirements and that these functions are mediated via the Tim-1 receptor. H-ferritin can block Sema4A-mediated cytotoxicity. Sema4A is detectable in the CSF of multiple sclerosis patients and HIV-seropositive persons and can induce oligodendrocyte cell death. PMID: 29457657
Advanced peri-implantitis lesions showed higher levels of gene expression for Sem3A and Sem4D and lower levels of Sem4A in comparison to tissues obtained from a healthy dental implant. PMID: 29763494
Here we show three families with retinal degeneration in which unaffected family members are either homozygous or heterozygous for the variant. The p.R713Q variant in SEMA4A is insufficient to cause either autosomal recessive or autosomal dominant retinitis pigmentosa and is unlikely to be pathogenic. PMID: 28805479
ILT-4 is a receptor for hSEMA4A)on activated CD4(+) T cells. hSEMA4A is highly expressed in human asthmatic lung tissue. PMID: 29467366
Suggest a role for Plexin-B1 as a ligand and Sema4A as a receptor and characterize a reverse signaling pathway downstream of Sema4A regulating cell migration via Scrib. PMID: 28007914
SEMA 4A confers doxorubicin resistance on hepatocellular carcinoma by inducing epithelialemesenchymal transition. PMID: 27697528
In this review, we summarized the current findings on neuroimmune Sema4A and Sema4D molecules in chronic inflammation underlying many diseases and discussed their positive or negative impacts on the implicated molecular and cellular processes PMID: 27554682
Sema4A activated the Akt pathway via Plexin D1 receptor in lung fibroblasts.Lung fibroblasts show elevated levels of Sema4A expression in systemic sclerosis patients. PMID: 26648031
Data suggest that increased expression of semaphorin 4A (Sema4A) is required to promote inflammation of rheumatoid arthritis (RA). PMID: 26303122
Germline variants in SEMA4A predispose to familial colorectal cancer type X. PMID: 25307848
PACAP released from retinal neural cells (photoreceptors or optic nerve cells) may regulate Sema4A expression in retinal pigment epithelial cells and thereby contribute to the maintenance of retinal structure and function. PMID: 25515530
These results indicate the importance of the Sema4A protein conformation in human and mouse retina homeostasis. PMID: 23360997
Sema4A plays an inhibitory role in T helper (Th)2-type allergic diseases, such as allergic asthma, in a fraction of Sema4a-deficient transgenic mice. PMID: 23007237
mutations in SEMA4A may cause susceptibility to light exposure, oxidative stress, and ER stress, which may be involved in the progression and pathology of RP. PMID: 22956603
Dendritic cell-derived SEM4A is not only critical for T helper type (Th)1 cells but also for Th17 cell differentiation, and multiple sclerosis patients with high Sema4A levels exhibit Th17 cell skewing. PMID: 22491253
Mutations within the SEMA4A gene cause various retinal degnerative diseases. PMID: 16199541

Hide All

Involvement in disease

Retinitis pigmentosa 35 (RP35); Cone-rod dystrophy 10 (CORD10)

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

Semaphorin family

Database Links

HGNC: 10729

OMIM: 607292

KEGG: hsa:64218

STRING: 9606.ENSP00000347117

UniGene: Hs.408846

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Code	CSB-EL020987HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.