Human Semenogelin-2(SEMG2) ELISA kit

Code CSB-EL021002HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q02383
Lead Time 7-14 working days
Abbreviation SEMG2
Target Name semenogelin II
Alias SGII, Semenogelin 2
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details The secreted protein encoded by this gene is involved in the formation of a gel matrix that encases ejaculated spermatozoa. Proteolysis by the prostate-specific antigen (PSA) breaks down the gel matrix and allows the spermatozoa to move more freely. The encoded protein is found in lesser abundance than a similar semenogelin protein. The genes encoding these two semenogelin proteins are found in a cluster on chromosome 20.
HGNC 10743
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SEMG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 85-94
1:2 Average % 92
Range % Sep-87
1:4 Average % 92
Range % 85-96
1:8 Average % 89
Range % 87-92
Recovery
The recovery of human SEMG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 86-96
EDTA plasma (n=4) 103 98-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.423 2.579 2.501 2.393
1000 1.954 2.054 2.004 1.896
500 1.265 1.302 1.284 1.176
250 0.712 0.734 0.723 0.615
125 0.416 0.405 0.411 0.303
62.5 0.251 0.266 0.259 0.151
31.25 0.176 0.183 0.180 0.072
0 0.107 0.108 0.108  
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Function Participates in the formation of a gel matrix (sperm coagulum) entrapping the accessory gland secretions and ejaculated spermatozoa.
Subcellular Location Secreted
Protein Families Semenogelin family
Tissue Specificity Seminal vesicles, and to a much lesser extent, epididymis.
Database Links

HGNC: 10743

OMIM: 182141

KEGG: hsa:6407

STRING: 9606.ENSP00000361855

UniGene: Hs.537218

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