Product Details

Target Name

serine peptidase inhibitor, Kazal type 6

Alternative Names

Acrosin inhibitor ELISA Kit; BUSI2 ELISA Kit; EG433180 ELISA Kit; ISK6_HUMAN ELISA Kit; MGC21394 ELISA Kit; Protease inhibitor H ELISA Kit; Serine protease inhibitor Kazal-type 6 ELISA Kit; Spink5l1 ELISA Kit; SPINK6 ELISA Kit; UNQ844 ELISA Kit; UNQ844/PRO1782 ELISA Kit

Abbreviation

SPINK6

Uniprot No.

Q6UWN8

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.625 ng/mL-40 ng/mL

Sensitivity

0.156 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SPINK6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	98
	Range %	94-102
1:4	Average %	102
	Range %	98-106
1:8	Average %	95
	Range %	90-100

Recovery

The recovery of human SPINK6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-106
EDTA plasma (n=4)	104	88-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	1.712	1.812	1.762	1.626
20	1.352	1.366	1.359	1.223
10	0.966	0.954	0.960	0.824
5	0.587	0.598	0.593	0.457
2.5	0.463	0.448	0.456	0.320
1.25	0.323	0.345	0.334	0.198
0.625	0.217	0.221	0.219	0.083
0	0.135	0.136	0.136

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	Serine protease inhibitor selective for kallikreins. Efficiently inhibits KLK4, KLK5, KLK6, KLK7, KLK12, KLK13 and KLK14. Doesn't inhibit KLK8.
Gene References into Functions	Study showed that the expression of SPINK6 is specifically suppressed in liver tumor tissues. The suppression could be even detected in the early stage tumors. As SPINK6 is a secretory protein, the extracellular protein level may be decreased because of tumor development. These results support that SPINK6 is an important tumor suppressor in liver. PMID: 27999203 our results identified a novel EGFR-activating mechanism in which SPINK6 has a critical role in promoting nasopharyngeal carcinoma metastasis PMID: 27671677 a significant fraction of SPINK6-sensitive proteases in healthy saliva and confirmed the ability of gingipains to inactivate SPINK6 under ex vivo conditions PMID: 27354280 KLKs. Thereby, beside the conserved binding mode, we identified an alternate binding mode which has so far not been observed for SPINK inhibitors. PMID: 26828269 Our study indicates that Spink6 is a potent inhibitor of kallikrein related peptidases and involved in skin barrier function. PMID: 24352040 Cross-linked SPINK6 might protect specific substrates such as fibronectin from kallikrein-related peptidases cleavage and contribute to the regulation of proteases in the epidermis. PMID: 23303447 Serine protease of Kazal-type (SPINK6) expressed in normal human skin is a potent natural inhibitor of Kallikrein-related peptidases, KLK12 and KLK13. PMID: 21439340 Data suggest that SPINK6 plays a role in modulating the activity of kallikreins in human skin. PMID: 20667819 Show More Hide All
Subcellular Location	Secreted.
Database Links	HGNC: 29486 OMIM: 615868 KEGG: hsa:404203 STRING: 9606.ENSP00000324870 UniGene: Hs.334274

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Check Code：

Code	CSB-EL022579HU
Size	96T,5×96T,10×96T
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Human Serine protease inhibitor Kazal-type 6(SPINK6) ELISA kit

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