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Human Serine protease inhibitor Kazal-type 6(SPINK6) ELISA kit

Instructions
Code CSB-EL022579HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name serine peptidase inhibitor, Kazal type 6
Alternative Names Acrosin inhibitor ELISA Kit; BUSI2 ELISA Kit; EG433180 ELISA Kit; ISK6_HUMAN ELISA Kit; MGC21394 ELISA Kit; Protease inhibitor H ELISA Kit; Serine protease inhibitor Kazal-type 6 ELISA Kit; Spink5l1 ELISA Kit; SPINK6 ELISA Kit; UNQ844 ELISA Kit; UNQ844/PRO1782 ELISA Kit
Abbreviation SPINK6
Uniprot No. Q6UWN8
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SPINK6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1 Average % 87
Range % 82-93
1:2 Average % 98
Range % 94-102
1:4 Average % 102
Range % 98-106
1:8 Average % 95
Range % 90-100
Recovery
The recovery of human SPINK6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 85-106
EDTA plasma (n=4) 104 88-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
40 1.712 1.812 1.762 1.626
20 1.352 1.366 1.359 1.223
10 0.966 0.954 0.960 0.824
5 0.587 0.598 0.593 0.457
2.5 0.463 0.448 0.456 0.320
1.25 0.323 0.345 0.334 0.198
0.625 0.217 0.221 0.219 0.083
0 0.135 0.136 0.136 　
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
Serine protease inhibitor selective for kallikreins. Efficiently inhibits KLK4, KLK5, KLK6, KLK7, KLK12, KLK13 and KLK14. Doesn't inhibit KLK8.
Gene References into Functions
  1. Study showed that the expression of SPINK6 is specifically suppressed in liver tumor tissues. The suppression could be even detected in the early stage tumors. As SPINK6 is a secretory protein, the extracellular protein level may be decreased because of tumor development. These results support that SPINK6 is an important tumor suppressor in liver. PMID: 27999203
  2. our results identified a novel EGFR-activating mechanism in which SPINK6 has a critical role in promoting nasopharyngeal carcinoma metastasis PMID: 27671677
  3. a significant fraction of SPINK6-sensitive proteases in healthy saliva and confirmed the ability of gingipains to inactivate SPINK6 under ex vivo conditions PMID: 27354280
  4. KLKs. Thereby, beside the conserved binding mode, we identified an alternate binding mode which has so far not been observed for SPINK inhibitors. PMID: 26828269
  5. Our study indicates that Spink6 is a potent inhibitor of kallikrein related peptidases and involved in skin barrier function. PMID: 24352040
  6. Cross-linked SPINK6 might protect specific substrates such as fibronectin from kallikrein-related peptidases cleavage and contribute to the regulation of proteases in the epidermis. PMID: 23303447
  7. Serine protease of Kazal-type (SPINK6) expressed in normal human skin is a potent natural inhibitor of Kallikrein-related peptidases, KLK12 and KLK13. PMID: 21439340
  8. Data suggest that SPINK6 plays a role in modulating the activity of kallikreins in human skin. PMID: 20667819

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Subcellular Location Secreted
Database Links

HGNC: 29486

OMIM: 615868

KEGG: hsa:404203

STRING: 9606.ENSP00000324870

UniGene: Hs.334274
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