Human Signal Peptide, CUB and EGF-like Domain-containing Protein 1(SCUBE1)ELISA Kit

Instructions
Code CSB-E15005h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name signal peptide, CUB domain, EGF-like 1
Alternative Names SCUB1_HUMAN ELISA Kit; SCUBE 1 ELISA Kit; SCUBE1 ELISA Kit; Signal peptide, CUB and EGF-like domain-containing protein 1 ELISA Kit; Signal peptide, CUB domain, EGF like 1 ELISA Kit
Abbreviation SCUBE1
Uniprot No. Q8IWY4
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SCUBE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:5Average %89
Range %82-94
1:10Average %96
Range %91-105
1:20Average %99
Range %95-108
1:40Average %94
Range %86-102
Recovery
The recovery of human SCUBE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10699-110
EDTA plasma (n=4)9284-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
402.670 2.592 2.631 2.502
202.271 2.173 2.222 2.093
101.631 1.615 1.623 1.494
50.940 0.925 0.933 0.804
2.50.512 0.502 0.507 0.378
1.250.357 0.347 0.352 0.223
0.6250.226 0.219 0.223 0.094
00.130 0.127 0.129  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.
Gene References into Functions
  1. The results of this pioneering study indicate that SCUBE protein family appears to have a probable role in the pathogenesis and angiogenesis development in psoriasis and SCUBE 1 and 3 may be novel markers of angiogenesis in psoriasis. PMID: 28238185
  2. enhanced SCUBE1 concentrations are correlated with increasing severity and poor outcomes of aneurysmal subarachnoid hemorrhage patients, indicating SCUBE1 might have the potential to identify aneurysmal subarachnoid hemorrhage patients at risk of poor prognosis PMID: 27265525
  3. Increased serum SCUBE1 concentrations have close relation to increasing severity and poor prognosis of intracerebral hemorrhage PMID: 27496080
  4. SCUBE1 might be a novel prognostic biomarker after traumatic brain injury PMID: 26940951
  5. describe novel mechanisms in targeting S1 to the plasma membrane and demonstrate that N-glycans are required for S1 functions during primitive haematopoiesis in zebrafish. PMID: 26699903
  6. SCUBE1 may indicate hypercoagulability in patients with BC and thus help identify patients at greater risk of thrombosisSCUBE1 may indicate hypercoagulability in patients with breast cancer and thus help identify patients at greater risk of thrombosis PMID: 26438214
  7. SCUBE1 levels are a new biomarker that can be used in the differential diagnosis and monitoring of patients hospitalized with suspected CCHF. PMID: 23632301
  8. Primary prostatic cancer-associated fibroblasts (CAFs) were isolated and the expression of CAF markers, Dlk1 and SCUBE1 transcripts were characterised. PMID: 23136397
  9. SCUBE 1 levels in patients with gastric cancer were found higher compared to healthy subjects. PMID: 23220851
  10. SCUBE1 level, a potential acute ischemia marker, is elevated in hemodialysis patients with no clinical ischemic event. PMID: 22874483

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Subcellular Location Secreted, Cell membrane, Peripheral membrane protein
Tissue Specificity Detected in endothelial cells. Highly expressed in platelets. Stored in platelet alpha granules, and transferred to the cell surface upon activation and aggregation. A smaller form, probably produced by limited proteolysis, after being released from the s
Database Links

HGNC: 13441

OMIM: 611746

KEGG: hsa:80274

STRING: 9606.ENSP00000354080

UniGene: Hs.133995

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