Human Signal Peptide, CUB and EGF-like Domain-containing Protein 1(SCUBE1)ELISA Kit

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Human Signal Peptide, CUB and EGF-like Domain-containing Protein 1(SCUBE1)ELISA Kit

Citations (18) Protocol

Code	CSB-E15005h
Size	96T,5×96T,10×96T
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Product Details
Citations (18)
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Description

This Human SCUBE1 ELISA Kit was designed for the quantitative measurement of Human SCUBE1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .

Target Name

signal peptide, CUB domain, EGF-like 1

Alternative Names

SCUB1_HUMAN ELISA Kit; SCUBE 1 ELISA Kit; SCUBE1 ELISA Kit; Signal peptide, CUB and EGF-like domain-containing protein 1 ELISA Kit; Signal peptide, CUB domain, EGF like 1 ELISA Kit

Abbreviation

SCUBE1

Uniprot No.

Q8IWY4

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.625 ng/mL-40 ng/mL

Sensitivity

0.156 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SCUBE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	89
	Range %	82-94
1:10	Average %	96
	Range %	91-105
1:20	Average %	99
	Range %	95-108
1:40	Average %	94
	Range %	86-102

Recovery

The recovery of human SCUBE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	106	99-110
EDTA plasma (n=4)	92	84-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.670	2.592	2.631	2.502
20	2.271	2.173	2.222	2.093
10	1.631	1.615	1.623	1.494
5	0.940	0.925	0.933	0.804
2.5	0.512	0.502	0.507	0.378
1.25	0.357	0.347	0.352	0.223
0.625	0.226	0.219	0.223	0.094
0	0.130	0.127	0.129

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

The diagnostic potential of plasma SCUBE‐1 concentration for pulmonary embolism: A pilot study L Xiao,The clinical respiratory journal,2023
CAFs-derived SCUBE1 promotes malignancy and stemness through the Shh/Gli1 pathway in hepatocellular carcinoma J Zhao,Journal of translational medicine,2022
Can serum SCUBE1 levels be useful in the diagnosis of bladder cancer? A MENTEŞE,Journal of Experimental and Clinical Medicine,2022
Pericardial SCUBE1 levels may help predict postoperative results in patients operated on for coronary artery bypass graft surgery T Özkara,CARDIOVASCULAR JOURNAL OF AFRICA,2021
Diagnostic value of signal peptide-Complement C1r/C1s, Uegf, and Bmp1-epidermal growth factor domain-containing protein 1 on serum and tissue samples in non-small cell lung cancer Selcuk Gurz.et al,/,2018
The Diagnostic Value of Scube1 in Patients Presenting to the Emergency Department Due to Angina Pectoris Aynur SAHIN.et al,/,2018
The predictive value of signal peptide-CUB-EGF domain-containing protein1 (SCUBE1) in terms of the duration of peripheral ischemia and exposure to reperfusion Ozgur Sogut.et al,Biomed Res Int.,2016
Increased serum concentrations of signal peptide-Cub-Egf domain-containing protein-1 in patients with aneurysmal subarachnoid hemorrhage. Ding YS.et al,Clin Chim Acta.,2016
Role of signal peptide-Cub-Egf domain-containing protein-1 in serum as a predictive biomarker of outcome after severe traumatic brain injury Chen QH.et al,Clin Chim Acta.,2016
Evaluation of SCUBE-1 and sCD40L biomarkers in patients with hypothyroidism due to Hashimoto's thyroiditis: a single-blind, controlled clinical study Bilir B.et al,Eur Rev Med Pharmacol Sci.,2016
The Diagnostic Value of Plasma SCUBE1, a Novel Biomarker of Platelet Activation, in Testicular Torsion: A Randomized, Controlled, Experimental Study Turedi S.et al,Urology,2015
Elevated Serum Levels of SCUBE1, aMarker for Coagulation, in Patients with Breast Cancer Topcu TO.et al,Tohoku J Exp Med.,2015
The role of hypoxia at primary dysmenorrhea, utilizing a novel hypoxiaMarker--SCUBE1. Tekin YB.et al,J Pediatr Adolesc Gynecol.,2015
The diagnostic role of signal peptide-C1r/C1s, Uegf, and Bmp1-epidermal growth factor domain-containing protein 1 in non-ST-elevation acute coronary syndrome. Sonmez E.et al,Am J Emerg Med.,2015
Signal peptide-UB-GF domain-containing protein 1 (SCUBE1) levels in patients with overt and subclinical hyperthyroidism: effects of treatment Erem C. et al,Clin Endocrinol (Oxf),2015
The diagnostic value of SCUBE1 levels in acute ischemic stroke Muumlcahit Guumlnaydin et al,Türk Biyokimya Dergisi,2014
The diagnostic and prognostic significance of SCUBE1 levels in Crimean-Congo hemorrhagic fever Mentese A et al,Int J Infect Dis.,2013
Signal peptide-CUB-EGF domain-containing protein 1 (SCUBE1) level in hemodialysis patients and parameters affecting that level /,Clinical Biochemistry,2012

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Function (From Uniprot)	Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.
Gene References into Functions	The results of this pioneering study indicate that SCUBE protein family appears to have a probable role in the pathogenesis and angiogenesis development in psoriasis and SCUBE 1 and 3 may be novel markers of angiogenesis in psoriasis. PMID: 28238185 enhanced SCUBE1 concentrations are correlated with increasing severity and poor outcomes of aneurysmal subarachnoid hemorrhage patients, indicating SCUBE1 might have the potential to identify aneurysmal subarachnoid hemorrhage patients at risk of poor prognosis PMID: 27265525 Increased serum SCUBE1 concentrations have close relation to increasing severity and poor prognosis of intracerebral hemorrhage PMID: 27496080 SCUBE1 might be a novel prognostic biomarker after traumatic brain injury PMID: 26940951 describe novel mechanisms in targeting S1 to the plasma membrane and demonstrate that N-glycans are required for S1 functions during primitive haematopoiesis in zebrafish. PMID: 26699903 SCUBE1 may indicate hypercoagulability in patients with BC and thus help identify patients at greater risk of thrombosisSCUBE1 may indicate hypercoagulability in patients with breast cancer and thus help identify patients at greater risk of thrombosis PMID: 26438214 SCUBE1 levels are a new biomarker that can be used in the differential diagnosis and monitoring of patients hospitalized with suspected CCHF. PMID: 23632301 Primary prostatic cancer-associated fibroblasts (CAFs) were isolated and the expression of CAF markers, Dlk1 and SCUBE1 transcripts were characterised. PMID: 23136397 SCUBE 1 levels in patients with gastric cancer were found higher compared to healthy subjects. PMID: 23220851 SCUBE1 level, a potential acute ischemia marker, is elevated in hemodialysis patients with no clinical ischemic event. PMID: 22874483 Show More Hide All
Subcellular Location	Secreted. Cell membrane; Peripheral membrane protein.
Tissue Specificity	Detected in endothelial cells. Highly expressed in platelets. Stored in platelet alpha granules, and transferred to the cell surface upon activation and aggregation. A smaller form, probably produced by limited proteolysis, after being released from the s
Database Links	HGNC: 13441 OMIM: 611746 KEGG: hsa:80274 STRING: 9606.ENSP00000354080 UniGene: Hs.133995

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ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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