Human Small Dense Low Density Lipoprotein(sLDL)ELISA Kit

Code CSB-E13350h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human sLDL ELISA Kit was designed for the quantitative measurement of Human sLDL protein in serum, plasma, saliva, urine, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.312 nmol/mL-20 nmol/mL and the sensitivity is 0.078 nmol/mL.

Target Name Small Dense Low Density Lipoprotein(sLDL)
Abbreviation sLDL
Species Homo sapiens (Human)
Sample Types serum, plasma, saliva, urine, tissue homogenates
Detection Range 0.312 nmol/mL-20 nmol/mL
Sensitivity 0.078 nmol/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human sLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 95  
Range % 92-103  
1:200 Average % 92  
Range % 86-96  
1:400 Average % 104  
Range % 97-107  
1:800 Average % 84  
Range % 80-88  
Recovery
The recovery of human sLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 87 83-93  
EDTA plasma (n=4) 92 88-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
nmol/ml OD1 OD2 Average    
20 0.086 0.085 0.086    
10 0.177 0.181 0.179    
5 0.384 0.375 0.380    
2.5 0.718 0.704 0.711    
1.25 1.253 1.269 1.261    
0.625 1.728 1.714 1.721    
0.312 2.223 2.121 2.172    
0 2.526 2.425 2.476    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

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 Q&A
Q:

What kind of dsLDL does the kit measure? (LDL-cholesterol? LDL-ApoB…. Etc?)
How does the kit differentiate de sdLDL from other LDL?

A:
Thanks for your inquiry.
This kit specifically use the sLDL raw material and detects the total sLDL(including the free SLDL or in combination with other proteins).
sLDL is the small dense Low-Density Lipoprotein,sd LDL or sLDL, which has much smaller grains and lower densities.
While those larger grains and lower densities LDL called large LDL.
You also can check more detailed informaiton on sLDL from this link: https://www.sciencedirect.com/science/article/pii/S0167527399001072 Pls let me know if you have any further questions. Thank you.
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