Human Thioredoxin domain-containing protein 5(TXNDC5) ELISA kit

Product Details

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Alternative Names

EndoPDI ELISA Kit; Endoplasmic reticulum protein ERp46 ELISA Kit; Endoplasmic reticulum resident protein 46 ELISA Kit; Endothelial protein disulphide isomerase ELISA Kit; ER protein 46 ELISA Kit; ERp46 ELISA Kit; Hcc 2 ELISA Kit; MGC3178 ELISA Kit; PDIA15 ELISA Kit; Protein disulfide isomerase family A member 15 ELISA Kit; STRF8 ELISA Kit; Thioredoxin domain containing 5 (endoplasmic reticulum) ELISA Kit; Thioredoxin domain containing 5 ELISA Kit; Thioredoxin domain containing protein 5 ELISA Kit; Thioredoxin domain-containing protein 5 ELISA Kit; Thioredoxin like protein p46 ELISA Kit; Thioredoxin related protein ELISA Kit; Thioredoxin-like protein p46 ELISA Kit; TLP46 ELISA Kit; TXND5_HUMAN ELISA Kit; TXNDC 5 ELISA Kit; Txndc5 ELISA Kit

Abbreviation

TXNDC5

Uniprot No.

Q8NBS9

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TXNDC5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:5	Average %	90
	Range %	87-94
1:10	Average %	90
	Range %	85-94
1:20	Average %	88
	Range %	80-96
1:40	Average %	92
	Range %	86-99

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.286

2.186

2.236

2.083

1.752

1.652

1.702

1.549

2.5

1.193

1.153

1.173

1.020

1.25

0.704

0.694

0.699

0.546

0.625

0.441

0.431

0.436

0.283

0.312

0.311

0.301

0.306

0.153

0.156

0.236

0.226

0.231

0.078

0.153

0.152

0.153

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Target Background

Function
(From Uniprot)

Possesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide-isomerase deficiency in yeast.

Gene References into Functions

Hypoxia induces the expression of TXNDC5 via upregulating HIF-1alpha in colorectal cancer cells. PMID: 29749460
TXNDC5 is also closely associated with rheumatoid arthritis, diabetes, hepatic steatosis and vitiligo. [review] PMID: 28604976
High TXNDC5 expression is associated with renal cell carcinoma. PMID: 26810069
we defined an important role of TXNDC5 in CRPC and further investigations are needed to screen TXNDC5 antagonists as a novel therapeutic approaches to treat PCa patients with Castration-resistant prostate cancer. PMID: 25500540
Role of ERp46 in oxidative protein folding.Independent actions of ERp46 thioredoxin domains on folding substrates.Structure of thioredoxin ERp46 domains. PMID: 24462249
suggest that the tumorigenic properties of ERp46 in RCC cells are not related to an inhibitory modulation of AdipoR1 PMID: 24594673
Endo-PDI is a novel, important mediator of AP-1-driven gene expression and endothelial angiogenic function. PMID: 24103565
TXNDC5 has increased expression in many tumors that is involved in the proliferation and migration of tumor cells, acting as a tumor-enhancing gene. PMID: 24100949
results suggest that TXNDC5 polymorphisms could be related to the development of HCC in the Korean male population PMID: 24023338
Hyperoxidized peroxiredoxin 2 interacts with the protein disulfide- isomerase ERp46. PMID: 23713588
Hypoxia induced TXCNDC5 expression, which contributed to adiponectin expression, cytokine production and the cellular proliferation and migration of fibroblasts in rheumatoid arthritis. PMID: 23326410
Authors show that ERp46 is able to bind to a large pool of peptides containing aromatic and basic residues via all three of its catalytic domains (a(0), a and a'), though the a(0) domain may contain the primary binding site. PMID: 23376096
crystal structure of the third catalytic domain of protein disulfide isomerase ERp46 (also known as protein disulfide isomerase A5 and TXNDC5) was determined to 2.0 A resolution PMID: 22505402
TXNDC5 has a genetic effect on the risk of rheumatoid arthritis and ankylosing spondylitis PMID: 21801346
Overexpression of the TXNDC5 protein is associated with non-small cell lung carcinoma. PMID: 21617212
results suggest that TXNDC5 gene polymorphisms are associated with the development of nonsegmental vitiligo in the Korean population. PMID: 19906073
TXNDC5 can promote the growth and proliferation of gastric cells. Silencing of TXNDC5 can restrain the growth and proliferation of gastric cancer cells. PMID: 20157732
these results establish ERp46 as the first AdipoR1-specific interacting protein and suggest a role for ERp46 in adiponectin receptor biology and adiponectin signalling. PMID: 20074551
ERp46 has specificity towards peroxiredoxin-4, a thioredoxin peroxidase. PMID: 19887585
upregulation of TXNDC5 are involved in the early development of colorectal cancer PMID: 17895523
Member of the protein disulphide isomerase family and highly expressed in endothelial cells. Protects endothelial cells from apoptosis under hypoxia. PMID: 12963716

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Subcellular Location

Endoplasmic reticulum lumen.

Protein Families

Protein disulfide isomerase family

Database Links

HGNC: 21073

OMIM: 616412

KEGG: hsa:81567

STRING: 9606.ENSP00000369081

UniGene: Hs.150837

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Code	CSB-EL025379HU
Size	96T,5×96T,10×96T
Price	Request a Quote
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.