Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

TXNDC5

Uniprot No.

Q8NBS9

Research Area

Cell Biology

Alternative Names

EndoPDI; Endoplasmic reticulum protein ERp46; Endoplasmic reticulum resident protein 46; Endothelial protein disulphide isomerase; ER protein 46; ERp46; Hcc 2; MGC3178; PDIA15; Protein disulfide isomerase family A member 15; STRF8; Thioredoxin domain containing 5 (endoplasmic reticulum); Thioredoxin domain containing 5; Thioredoxin domain containing protein 5; Thioredoxin domain-containing protein 5; Thioredoxin like protein p46; Thioredoxin related protein; Thioredoxin-like protein p46; TLP46; TXND5_HUMAN; TXNDC 5; Txndc5

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-324aa

Target Protein Sequence

MEDAKVYVAKVDCTAHSDVCSAQGVRGYPTLKLFKPGQEAVKYQGPRDFQTLENWMLQTLNEEPVTPEPEVEPPSAPELKQGLYELSASNFELHVAQGDHFIKFFAPWCGHCKALAPTWEQLALGLEHSETVKIGKVDCTQHYELCSGNQVRGYPTLLWFRDGKKVDQYKGKRDLESLREYVESQLQRTETGATETVTPSEAPVLAAEPEADKGTVLALTENNFDDTIAEGITFIKFYAPWCGHCKTLAPTWEELSKKEFPGLAGVKIAEVDCTAERNICSKYSVRGYPTLLLFRGGKKVSEHSGGRDLDSLHRFVLSQAKDEL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

63.2kDa

Protein Length

Full Length of Isoform 2

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This Recombinant Human TXNDC5 protein is an essential tool for researchers in the field of cell biology and related studies. Thioredoxin domain-containing protein 5 (TXNDC5) is a multifunctional protein involved in various cellular processes, including protein folding, redox regulation, and cellular stress response. This versatile protein has been implicated in a variety of pathophysiological conditions, such as inflammation, autoimmune diseases, and cancer.

Our Recombinant Human TXNDC5 protein is produced using a highly efficient E.coli expression system, ensuring a properly folded and functional protein. The expression region of our TXNDC5 protein spans from 1-324aa, covering the full length of Isoform 2. For ease of purification and detection, an N-terminal GST-tag is included, guaranteeing a consistent and high-quality product for your research needs.

With a purity greater than 90% as determined by SDS-PAGE, our Recombinant Human TXNDC5 protein exhibits exceptional quality for your experiments. We offer this product in both liquid and lyophilized powder forms, providing you with the flexibility to choose the most suitable format for your research requirements. Rely on our Recombinant Human TXNDC5 protein to advance your cell biology research and achieve outstanding results.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

Can this protein be removed both of the His tag and Myc-tag?

Thanks for your inquiry.Please remark your requirement for tag removal if you need the untagged protein or tag removal when placing the order. We can try enzyme digestion, but we can't guarantee 100% successfully.
The overall success rate of enzyme digestion data analysis is 75%-86%.
a.Not all protein tags can be removed as some proteins will be very unstable after tag removal.
b.If we succeed in removing the tag, we will charge for some extra cost.
c.If we fail in removing the tag, we won’t charge for any extra cost, and remark this information in datasheet as follows
“Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
d.Generally, the delivery time will be extended for 3 working days.

Target Background

Function

Possesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide-isomerase deficiency in yeast.

Gene References into Functions

Hypoxia induces the expression of TXNDC5 via upregulating HIF-1alpha in colorectal cancer cells. PMID: 29749460
TXNDC5 is also closely associated with rheumatoid arthritis, diabetes, hepatic steatosis and vitiligo. [review] PMID: 28604976
High TXNDC5 expression is associated with renal cell carcinoma. PMID: 26810069
we defined an important role of TXNDC5 in CRPC and further investigations are needed to screen TXNDC5 antagonists as a novel therapeutic approaches to treat PCa patients with Castration-resistant prostate cancer. PMID: 25500540
Role of ERp46 in oxidative protein folding.Independent actions of ERp46 thioredoxin domains on folding substrates.Structure of thioredoxin ERp46 domains. PMID: 24462249
suggest that the tumorigenic properties of ERp46 in RCC cells are not related to an inhibitory modulation of AdipoR1 PMID: 24594673
Endo-PDI is a novel, important mediator of AP-1-driven gene expression and endothelial angiogenic function. PMID: 24103565
TXNDC5 has increased expression in many tumors that is involved in the proliferation and migration of tumor cells, acting as a tumor-enhancing gene. PMID: 24100949
results suggest that TXNDC5 polymorphisms could be related to the development of HCC in the Korean male population PMID: 24023338
Hyperoxidized peroxiredoxin 2 interacts with the protein disulfide- isomerase ERp46. PMID: 23713588
Hypoxia induced TXCNDC5 expression, which contributed to adiponectin expression, cytokine production and the cellular proliferation and migration of fibroblasts in rheumatoid arthritis. PMID: 23326410
Authors show that ERp46 is able to bind to a large pool of peptides containing aromatic and basic residues via all three of its catalytic domains (a(0), a and a'), though the a(0) domain may contain the primary binding site. PMID: 23376096
crystal structure of the third catalytic domain of protein disulfide isomerase ERp46 (also known as protein disulfide isomerase A5 and TXNDC5) was determined to 2.0 A resolution PMID: 22505402
TXNDC5 has a genetic effect on the risk of rheumatoid arthritis and ankylosing spondylitis PMID: 21801346
Overexpression of the TXNDC5 protein is associated with non-small cell lung carcinoma. PMID: 21617212
results suggest that TXNDC5 gene polymorphisms are associated with the development of nonsegmental vitiligo in the Korean population. PMID: 19906073
TXNDC5 can promote the growth and proliferation of gastric cells. Silencing of TXNDC5 can restrain the growth and proliferation of gastric cancer cells. PMID: 20157732
these results establish ERp46 as the first AdipoR1-specific interacting protein and suggest a role for ERp46 in adiponectin receptor biology and adiponectin signalling. PMID: 20074551
ERp46 has specificity towards peroxiredoxin-4, a thioredoxin peroxidase. PMID: 19887585
upregulation of TXNDC5 are involved in the early development of colorectal cancer PMID: 17895523
Member of the protein disulphide isomerase family and highly expressed in endothelial cells. Protects endothelial cells from apoptosis under hypoxia. PMID: 12963716

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Subcellular Location

Endoplasmic reticulum lumen.

Protein Families

Protein disulfide isomerase family

Database Links

HGNC: 21073

OMIM: 616412

KEGG: hsa:81567

STRING: 9606.ENSP00000369081

UniGene: Hs.150837

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