Human Thiosulfate sulfurtransferase(TST) ELISA kit

Code CSB-EL025197HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
thiosulfate sulfurtransferase (rhodanese)
Alternative Names
GlpE ELISA Kit; RDS ELISA Kit; Rhodanese ELISA Kit; Thiosulfate sulfurtransferase ELISA Kit; Thiosulfate sulfurtransferase GlpE ELISA Kit; THTR_HUMAN ELISA Kit; TST ELISA Kit
Abbreviation
TST
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 80-92  
1:2 Average % 95  
Range % 91-105  
1:4 Average % 100  
Range % 92-110  
1:8 Average % 92  
Range % 86-98  
Recovery
The recovery of human TST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 89-98  
EDTA plasma (n=4) 95 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.504 2.511 2.508 2.352  
500 2.131 2.052 2.092 1.936  
250 1.415 1.333 1.374 1.218  
125 0.759 0.744 0.752 0.596  
62.5 0.541 0.523 0.532 0.376  
31.2 0.337 0.323 0.330 0.174  
15.6 0.251 0.244 0.248 0.092  
0 0.159 0.153 0.156    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human TST ELISA Kit was designed for the quantitative measurement of Human TST protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

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Target Background

Function
(From Uniprot)
Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity. Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.
Gene References into Functions
  1. TST mRNA expression in adipose tissue correlated positively with insulin sensitivity in adipose tissue and negatively with fat mass. PMID: 27270587
  2. show that polymorphic variations that are distant from the active site differentially modulate the sulfurtransferase activity of human rhodanese to cyanide versus sulfite PMID: 26269602
  3. Loss of Thiosulfate Sulfurtransferase is associated with decreased mucosal sulfide detoxification capacity in patients with Crohn's disease PMID: 23399736
  4. silencing of the rhodanese gene caused not only a proportional decrease of 5 S rRNA import but also a general inhibition of mitochondrial translation, indicating the functional importance of the imported 5 S rRNA inside the organelle. PMID: 20663881
  5. Proteomics was used to study colonic epithelial aging, for differential proteins in the human normal colonic epithelial tissues from young and old people. Rack1, EF-Tu and Rhodanese, three validated differential proteins, were further investigated. PMID: 20099848
  6. A partially gluconoylated N-terminus of the His6-tagged molybdenum cofactor synthesis 3 (MOCS3)-rhodanese-like domain is identified which results in a heterogeneity of the protein but does not influence sulfurtransferase activity. PMID: 15910006
  7. The USP8 recognition domain of NRDP1 has a novel protein fold that interacts with a conserved peptide loop of the rhodanese domain. PMID: 17035239

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Subcellular Location
Mitochondrion matrix.
Database Links

HGNC: 12388

OMIM: 180370

KEGG: hsa:7263

STRING: 9606.ENSP00000249042

UniGene: Hs.474783

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