Thromboxane B2 (TX-B2) is a stable metabolite of thromboxane A2, a potent bioactive lipid mediator derived from arachidonic acid through the cyclooxygenase pathway. As the primary measurable end product of thromboxane A2 synthesis, TX-B2 serves as a reliable biomarker for thromboxane production and platelet activation. This prostanoid plays important roles in cardiovascular physiology and pathology, making it a valuable target for research examining inflammation, thrombosis, and vascular function.
The Human Thromboxane B2,TX-B2 ELISA Kit (CSB-E08046h) uses a quantitative sandwich measurement principle for detecting TX-B2 in serum, plasma, cell culture supernates, and tissue homogenates. The assay provides a detection range of 9.38 pg/mL to 600 pg/mL with a sensitivity of 2.34 pg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in research studies to measure thromboxane B2 levels in human serum samples, serving as a stable biomarker for thromboxane A2 activity in various physiological and pathological conditions. The kit has shown reliable performance in studies examining vasoactive biomarkers and platelet function, where thromboxane B2 serves as an important indicator of cyclooxygenase pathway activity.
• Vasoactive biomarker research - Used to quantify thromboxane B2 as a stable metabolite reflecting thromboxane A2 production in cardiovascular and vascular function studies
• Platelet function analysis - Applied in measuring thromboxane B2 levels in both serum and platelet supernatant samples to evaluate platelet activation and aggregation pathways
• Multi-biomarker profiling - Incorporated into comprehensive biomarker panels alongside other inflammatory and vascular markers to evaluate complex physiological responses
• Serum-based studies - Used for routine measurement of circulating thromboxane B2 levels in human subjects as part of broader research examining prostaglandin metabolism
