Human Twisted gastrulation protein homolog 1(TWSG1) ELISA kit

Code CSB-EL025361HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
twisted gastrulation homolog 1 (Drosophila)
Alternative Names
PSEC0250 ELISA Kit; twisted gastrulation homolog 1 (Drosophila) ELISA Kit; TSG ELISA Kit; TSG1 ELISA Kit; Twisted gastrulation BMP signaling modulator 1 ELISA Kit; Twisted gastrulation homolog 1 ELISA Kit; Twisted gastrulation protein ELISA Kit; Twisted gastrulation protein homolog 1 ELISA Kit; TWSG1 ELISA Kit; TWSG1_HUMAN ELISA Kit
Abbreviation
TWSG1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
125 pg/mL-8000 pg/mL
Sensitivity
31.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TWSG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %86-96
1:2Average %99
Range %92-106
1:4Average %89
Range %83-94
1:8Average %92
Range %87-98
Recovery
The recovery of human TWSG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-99
EDTA plasma (n=4)8480-88
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
80002.557 2.765 2.661 2.542
40001.816 1.902 1.859 1.740
20001.103 1.189 1.146 1.027
10000.707 0.714 0.711 0.592
5000.405 0.374 0.390 0.271
2500.312 0.309 0.311 0.192
1250.205 0.201 0.203 0.084
00.123 0.115 0.119  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human TWSG1 ELISA Kit was designed for the quantitative measurement of Human TWSG1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL .

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Target Background

Function
(From Uniprot)
May be involved in dorsoventral axis formation. Seems to antagonize BMP signaling by forming ternary complexes with CHRD and BMPs, thereby preventing BMPs from binding to their receptors. In addition to the anti-BMP function, also has pro-BMP activity, partly mediated by cleavage and degradation of CHRD, which releases BMPs from ternary complexes. May be an important modulator of BMP-regulated cartilage development and chondrocyte differentiation. May play a role in thymocyte development.
Gene References into Functions
  1. Findings indicate that twisted gastrulation homolog 1 (TWSG1) maybe serves as a tumor suppressor gene in gastric cancer and potential biomarker or therapeutic target for the diagnosis and treatment of gastric cancer. PMID: 29756996
  2. Tsg functions as a BMP-agonist by inducing conformational change in chordin making it more susceptible to tolloid cleavage and as a BMP-antagonist either independently or via a chordin-mediated mechanism. PMID: 26829466
  3. Data suggest CHRD (chordin) cleavage by tolloid-like metalloproteinases is insufficient to regulate BMP(bone morphogenetic protein)/BMPR signaling; CHRD fragments and TSG (twisted gastrulation protein) contribute to inhibition of BMP/BMPR signaling. PMID: 26517884
  4. these results suggest that BMPER and Tsg maintain a fine-tuned equilibrium that controls BMP pathway activity and is necessary for vascular cell homeostasis. PMID: 23641068
  5. mutations in the twisted gastrulation homolog 1 gene are neither a common direct cause nor a frequent modifying factor for human holoprosencephaly pathologies PMID: 21227728
  6. Tsg functions as an agonist synergizing with TGF-beta to inhibit T-cell activation. PMID: 17164348
  7. interferes with BMP-mediated hepcidin expression and may act with GDF15 to dysregulate iron homeostasis in thalassemia syndromes. PMID: 19414861

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Subcellular Location
Secreted.
Protein Families
Twisted gastrulation protein family
Database Links

HGNC: 12429

OMIM: 605049

KEGG: hsa:57045

STRING: 9606.ENSP00000262120

UniGene: Hs.514685

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