Human Vitamin D Receptor,VD R ELISA Kit

Instructions
Code CSB-E05136h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The Human Vitamin D Receptor (VDR) ELISA Kit can quantitatively detect the content of VDR in human serum, plasma, tissue homogenates, or cell lysates. VDR is a member of the nuclear receptor/steroid hormone receptor superfamily. It is the single known regulatory mediator of hormonal 1,25-dihydroxy vitamin D3 [1,25(OH)2D3] in higher vertebrates. It acts in the nucleus of vitamin D target cells to regulate the expression of genes whose products control diverse, cell type-specific biological functions that include mineral homeostasis. VDR is also involved in the regulation of calcium and phosphate homeostasis in tissues, including the intestine, bone, kidney, and parathyroid glands. Importantly, It participates in bone development.

This assay employs the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction to achieve the measurement. The solution develops in proportion to the amount of VDR in the sample. And the color intensity can be measured at 450 nm through a microplate reader. The kit has undergone rigorous quality validation in many aspects, including sensitivity, specificity, precision, recovery, linearity, and consistency between batches.

Target Name vitamin D (1,25- dihydroxyvitamin D3) receptor
Alternative Names 1 25 dihydroxyvitamin D3 receptor ELISA Kit; 1 ELISA Kit; 1,25 dihydroxyvitamin D3 receptor ELISA Kit; 1,25-@dihydroxyvitamin D3 receptor ELISA Kit; 25-dihydroxyvitamin D3 receptor ELISA Kit; Member 1 ELISA Kit; NR1I1 ELISA Kit; Nuclear receptor subfamily 1 group I member 1 ELISA Kit; PPP1R163 ELISA Kit; Protein phosphatase 1, regulatory subunit 163 ELISA Kit; VDR ELISA Kit; VDR_HUMAN ELISA Kit; Vitamin D (1,25- dihydroxyvitamin D3) receptor ELISA Kit; Vitamin D hormone receptor ELISA Kit; Vitamin D nuclear receptor variant 1 ELISA Kit; Vitamin D receptor ELISA Kit; Vitamin D3 receptor ELISA Kit
Abbreviation VDR
Uniprot No. P11473
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human VD R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 87-96  
1:2 Average % 103  
Range % 97-105  
1:4 Average % 95  
Range % 90-100  
1:8 Average % 94  
Range % 88-98  
Recovery
The recovery of human VD R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 82-94  
EDTA plasma (n=4) 99 90-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
400 2.178 2.098 2.138 1.977  
200 1.562 1.644 1.603 1.442  
100 1.138 1.118 1.128 0.967  
50 0.811 0.803 0.807 0.646  
25 0.452 0.460 0.456 0.295  
12.5 0.325 0.315 0.320 0.159  
6.25 0.227 0.231 0.229 0.068  
0 0.162 0.160 0.161    
Materials provided
  • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-human VDR antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labled VDR antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
  • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
  • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Recruited to promoters via its interaction with BAZ1B/WSTF which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
Gene References into Functions
  1. Vitamin D Receptor Gene SNPs and the environment interact to Influence survival in hemodialysis patients. PMID: 30087217
  2. Results suggest the association between some maternal VDR polymorphisms with neonatal anthropometric measures and the risk of premature birth. PMID: 30150529
  3. SNPs of the VDR and GC genes are associated with vitamin D deficiency in postmenopausal Mexican women. PMID: 30150596
  4. VDR gene FokI polymorphism is associated with papillary thyroid cancer. PMID: 30486759
  5. No significant associations were found between the VDR polymorphisms analysed and Developmental dysplasia of the hip . Further work need to be performed using genomewide analysis to elucidate the genetic basis of Developmental dysplasia of the hip . PMID: 30262704
  6. There was no significant association detected between BMI and rs1544410 of VDR in the Emirati population PMID: 29343214
  7. Apparently, VDR-mediated signaling pathways seem to be dysregulated in those pathological conditions PMID: 30096760
  8. Vitamin D Receptorgene TaqI and BsmI polymorphisms might contribute to the increased risk of hallux valgus in Chinese population. Apal or Fokl polymorphisms showed no increased susceptibility. PMID: 29705233
  9. PTPN2, an anti-inflammatory factor regulated by VDR, was reduced in type 2 diabetics with chronic kidney disease stages 1-2. PMID: 30246029
  10. ApaI gene polymorphism and Fok1 FF genotype were associated with renal cell carcinoma susceptibility in Asians PMID: 29970659
  11. findings show polymorphism Taq-1 occurring in the vitamin D receptor may have an impact on the development of acute pancreatitis due to the lack of the protective role of vitamin D. PMID: 29966312
  12. only VDR FokI polymorphism is associated with Hashimoto's thyroiditis risk in Asian population, but not in Caucasians; and the TaqI, ApaI and BsmI polymorphisms have not positive association neither in the overall population (Meta-Analysis) PMID: 28134349
  13. Loss of function VDR mutation is associated with Hereditary 1,25-dihydroxyvitamin D-resistant rickets. PMID: 29949513
  14. JNK1 and VDR act as tumor suppressors, and their stromal expression levels are associated with prognosis in esophageal squamous cell carcinoma. PMID: 29423673
  15. Associations between VDR gene polymorphisms and osteoporosis risk and bone mineral density in postmenopausal women have been documented. (Meta-analysis) PMID: 29343720
  16. Vitamin D deficiency and vitamin D receptor variants in mothers and their neonates are risk factors for neonatal sepsis PMID: 29530503
  17. Study identified that CCC and TCC VDR haplotypes are risk factors for diabetic nephropathy in patients with diabetes type 2. PMID: 30315926
  18. The VDR rs2228570 variant may increase susceptibility to dyslipidemia in the Chinese Han population. PMID: 30119682
  19. NB-UVB phototherapy is associated with improved cutaneous VDR expression and vitamin D synthesis. Better repigmentation response to NB-UVB may be related to higher baseline VDR expression and its upregulation after phototherapy PMID: 29080365
  20. Vitamin D Receptor Gene Polymorphism is associated with Breast Cancer. PMID: 28780723
  21. Studied association of vitamin D receptor (VDR) single nucleotide polymorphisms (SNPs) and promoter region deletions of toll like receptor 2 (TLR2) with genetic predisposition for pulmonary tuberculosis (PTB) in India communities. Results show that the BsmI and FokI polymorphisms of the VDR gene are significantly associated with an increased risk of PTB. PMID: 29727015
  22. Results disclose FokI polymorphism as a relevant variant capturing the association of VDR polymorphisms with viral infection. PMID: 30092343
  23. VDR (rs1544410) SNP was found to be associated with decreased serum (25[OH]D) levels. PMID: 29738868
  24. CA genotype of ApaI VDR gene polymorphism was associated with family history and C allele of ApaI was related with family history and hypercalciuria in under one-year-old infants from Turkey. PMID: 29085969
  25. A total of six Bcell epitopes and three Tcell epitopes for VDR were predicted by bioinformatics, which when validated, may in the future aid in immunological diagnosis and development of a targeted drug therapy for clinical asthma. PMID: 29901144
  26. Review/Meta-analysis: VDR Tru9I polymorphism may be associated with osteoporosis risk in Chinese individuals, but BsmI, ApaI polymorphisms might not be a risk factor for osteoporosis. PMID: 29624920
  27. Our data reveal that VDR plays a central role in protecting cells from excessive respiration and production of ROS that leads to cell damage. PMID: 29874855
  28. This study emphasizes a positive association between SNPs (Fok-I and Bsm-I) and T1DM among Saudi children with increased risk with the Fok-I F and Bsm-I b alleles. PMID: 29417618
  29. The Apa-I variant in VDR gene is associated with metabolic syndrome in southern Brazilian women with polycystic ovary syndrome. PMID: 29669566
  30. Whole blood VDR gene expression was significantly higher in the autistic disorder group compared to control subjects (p < 0.0001). There were no significant differences among allele and genotype distribution of rs11568820 and rs4516035 polymorphisms between autistic disorder patients and controls. PMID: 29777458
  31. Vitamin D receptor ApaI AC genotype may be a possible cardiovascular risk factor for the development of arteriovenous fistula failure. PMID: 29544394
  32. preliminary results indicate the VDR gene ApaI, BsmI, FokI, and TaqI polymorphisms may not be associated with elevated multiple sclerosis (MS) risk among overall populations, but ApaI polymorphism may confer different susceptibility to MS among different populations - systematic review and meta-analysis PMID: 29110148
  33. Studied association between 25-hydroxy vitamin D (25[OH]D) levels and vitamin D receptor (VDR) gene polymorphism in association with diabetes type 2. PMID: 28739347
  34. The VDR Tru9I 'uu' genotype may increase the risk of premenopausal breast cancer. PMID: 29529900
  35. Low VDR expression is associated with Coronary Artery Disease. PMID: 29176261
  36. Expression analyses showed significant downregulation of VDR expression in peripheral blood of epileptic patients compared with healthy subjects. PMID: 29549592
  37. This meta-analysis demonstrated the association between FokI and ApaI polymorphisms in VDR gene with the risk of BD, providing insights into the potential role of vitamin D receptor in the pathogenesis of BD. PMID: 29388852
  38. Vitamin D receptor polymorphisms is a risk factor for multiple sclerosis susceptibility and progression in the Czech population. PMID: 29589202
  39. important role for SOST SNP rs1877632 and VDR SNPs rs10735810 and rs731236 in the pathophysiology of stress fracture PMID: 29129460
  40. CT genotype and the C allele of VDR were significantly associated with increased risks of childhood autism spectrum disorder. PMID: 29581796
  41. Study found a significant association between multiple sclerosis and the VDR FokI polymorphism in our region of Turkey. PMID: 29331875
  42. VDR's Fok-I and Taq-I show significant association with risk of RRMS, while Apa-I and Bsm-I are not related to the risk of the disease in Iranian Kurds. PMID: 29072967
  43. The VDR rs2228570 polymorphism increases the risk of ovarian cancer in Caucasian populations in a dominant genetic model. PMID: 29239065
  44. The present study indicates an association between VDR and vitamin D binding protein Single Nucleotide Polymorphisms and Type 1 Diabetes Mellitus among Turkish subjects. PMID: 29506625
  45. Review/Meta-analysis: VDR B allele, and BB + Bb genotypes of Bsm I variant, Tt genotype of Taq I variant might be risk factors for diabetic nephropathy. PMID: 28703918
  46. The VDR Bb genotype is an independent predictor of developing secondary hyperparathyroidism in patients with end stage kidney disease. PMID: 29415666
  47. FokI and TaqI VDR variants are significantly associated with systemic lupus erythematosus in an eastern Indian cohort. PMID: 29230954
  48. examination of the evidence for the role of Vitamin D Receptor (VDR) Polymorphisms in autoimmune diseases (review). PMID: 28786260
  49. results suggests that there may be a relationship between certain VDR genotype combinations and the risk of preterm birth. PMID: 27958635
  50. VDR BsmI polymorphism was associated with decreased risk of periodontitis in Chinese individuals from South China (meta-analysis). PMID: 29208185

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Involvement in disease Rickets vitamin D-dependent 2A (VDDR2A)
Subcellular Location Nucleus, Cytoplasm
Protein Families Nuclear hormone receptor family, NR1 subfamily
Database Links

HGNC: 12679

OMIM: 277440

KEGG: hsa:7421

STRING: 9606.ENSP00000447173

UniGene: Hs.524368

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