Human Vitamin D Receptor,VD R ELISA Kit

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Target Background

Name: Human Vitamin D Receptor,VD R ELISA Kit
Brand: CUSABIO
SKU: CSB-E05136h

Product Details

Target Name

vitamin D (1,25- dihydroxyvitamin D3) receptor

Alternative Names

1 25 dihydroxyvitamin D3 receptor ELISA Kit; 1 ELISA Kit; 1,25 dihydroxyvitamin D3 receptor ELISA Kit; 1,25-@dihydroxyvitamin D3 receptor ELISA Kit; 25-dihydroxyvitamin D3 receptor ELISA Kit; Member 1 ELISA Kit; NR1I1 ELISA Kit; Nuclear receptor subfamily 1 group I member 1 ELISA Kit; PPP1R163 ELISA Kit; Protein phosphatase 1, regulatory subunit 163 ELISA Kit; VDR ELISA Kit; VDR_HUMAN ELISA Kit; Vitamin D (1,25- dihydroxyvitamin D3) receptor ELISA Kit; Vitamin D hormone receptor ELISA Kit; Vitamin D nuclear receptor variant 1 ELISA Kit; Vitamin D receptor ELISA Kit; Vitamin D3 receptor ELISA Kit

Abbreviation

VDR

Uniprot No.

P11473

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

6.25 pg/mL-400 pg/mL

Sensitivity

1.56 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human VD R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-96
1:2	Average %	103
	Range %	97-105
1:4	Average %	95
	Range %	90-100
1:8	Average %	94
	Range %	88-98

Recovery

The recovery of human VD R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	82-94
EDTA plasma (n=4)	99	90-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.178	2.098	2.138	1.977
200	1.562	1.644	1.603	1.442
100	1.138	1.118	1.128	0.967
50	0.811	0.803	0.807	0.646
25	0.452	0.460	0.456	0.295
12.5	0.325	0.315	0.320	0.159
6.25	0.227	0.231	0.229	0.068
0	0.162	0.160	0.161

Materials provided

A micro ELISA plate --The 96-well plate has been pre-coated with an anti-human VDR antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labled VDR antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The vitamin D receptor (VDR) is a nuclear hormone receptor that mediates the biological effects of 1,25-dihydroxyvitamin D3, the active form of vitamin D. As a ligand-activated transcription factor, VDR regulates calcium homeostasis, bone mineralization, and immune function by controlling the expression of target genes involved in these processes. VDR is expressed in various tissues including intestine, kidney, prostate, and immune cells. This makes it a key mediator of vitamin D signaling pathways and an important research target for understanding metabolic bone diseases, autoimmune disorders, and cancer.

The Human Vitamin D Receptor ELISA Kit (CSB-E05136h) uses a quantitative sandwich assay principle to measure VDR levels in serum, plasma, tissue homogenates, and cell lysates. This kit offers a detection range of 6.25 pg/mL to 400 pg/mL with a sensitivity of 1.56 pg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours, with detection performed at 450 nm wavelength.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in clinical research studies to quantify vitamin D receptor levels in human biological samples as part of biomarker profiling. The kit supports studies examining vitamin D pathway components alongside other metabolic and inflammatory markers in various patient populations.

• Metabolic research: Measuring vitamin D receptor concentrations in studies that evaluate vitamin D status and related biochemical variables including glucose and lipid profiles
• Biomarker correlation studies: Quantifying vitamin D receptor levels alongside inflammatory markers and apoptosis-related proteins to examine potential associations in clinical populations
• Clinical profiling: Measuring vitamin D receptor in serum and plasma samples as part of multi-analyte panels that investigate endocrine and metabolic pathways in patient cohorts

Code	CSB-E05136h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.