Code | CSB-RA945260A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
The VDR recombinant monoclonal antibody is produced in four steps: (1) sequencing the VDR monoclonal antibody gene, (2) cloning the gene into a plasmid vector, (3) transfecting the VDR monoclonal antibody gene-carrying vector into a host cell line, and (4) purifying the VDR recombinant monoclonal antibody from the cell culture supernatant using affinity chromatography. The VDR monoclonal antibody is created by using a synthesized peptide derived from the human VDR protein as the immunogen. This recombinant monoclonal antibody is recommended for use in ELISA, WB, and IHC assays to detect human VDR protein.
The VDR mainly regulates gene expression in response to the hormone 1,25-dihydroxy vitamin D3 (1,25(OH)2D3), which is the active form of vitamin D3. Upon binding to 1,25(OH)2D3, VDR forms a complex with another protein, retinoid X receptor (RXR), and together they bind to specific regions of DNA called vitamin D response elements (VDREs) in the promoter region of target genes. This complex then recruits other proteins to activate or repress the transcription of the target genes, which leads to a wide range of biological effects, including calcium and phosphate metabolism, immune response, cell differentiation, and proliferation.
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