Human Zinc finger protein Aiolos(IKZF3) ELISA kit

Instructions
Code CSB-EL011577HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name IKAROS family zinc finger 3 (Aiolos)
Alternative Names AIO ELISA Kit; Aiolos ELISA Kit; IKAROS family zinc finger 3 (Aiolos) ELISA Kit; IKAROS family zinc finger 3 ELISA Kit; Ikaros family zinc finger protein 3 ELISA Kit; IKZF 3 ELISA Kit; IKZF3 ELISA Kit; IKZF3_HUMAN ELISA Kit; zinc finger DNA binding protein Aiolos ELISA Kit; Zinc finger protein Aiolos ELISA Kit; Zinc finger protein subfamily 1A 3 (Aiolos) ELISA Kit; Zinc finger protein subfamily 1A 3 ELISA Kit; Zinc finger protein subfamily 1A; member 3 ELISA Kit; ZNFN1A3 ELISA Kit
Abbreviation IKZF3
Uniprot No. Q9UKT9
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IKZF3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %86
Range %80-91
1:2Average %96
Range %91-104
1:4Average %96
Range %92-109
1:8Average %94
Range %86-97
Recovery
The recovery of human IKZF3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-97
EDTA plasma (n=4)9690-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
16002.098 1.986 2.042 1.950
8001.357 1.298 1.328 1.236
4000.825 0.789 0.807 0.715
2000.500 0.481 0.491 0.399
1000.360 0.351 0.356 0.264
500.245 0.240 0.243 0.151
250.156 0.152 0.154 0.062
00.092 0.091 0.092  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.
Gene References into Functions
  1. The minor alleles of rs2941522, rs907091, rs1453559, rs12150079 and rs2872507 were statistically associated with an increased risk in Graves' disease patients. PMID: 29510406
  2. Aiolos promotes the binding of Blimp-1 to target genes and thereby enhances Blimp-1-dependent transcriptional repression. PMID: 26823144
  3. Our data indicate a possible regulatory role for the multiple sclerosis-associated IKZF3 and IQGAP1 variants. PMID: 27080863
  4. results indicate that overexpression of Aiolos may contribute to pathogenesis of SLE and RA PMID: 26546109
  5. Aiolos improved the survival of Nalm6 cells via PTEN and Aktdependent processes. PMID: 25608224
  6. NFKB1, CD27, LAG3 and IKZF3 are new susceptibility genes for psoriasis. PMID: 25006012
  7. lentivirus-mediated AIOLOS overexpression in Jurkat cells induced cell apoptosis, arrested the cell cycle at the G0/G1 phase, and synergistically increased the sensitivity of Jurkat cells to etoposide by inhibiting NF-kappaB activity. PMID: 25524659
  8. identified a unique case of adult acute B-cell lymphoblastic leukemia with masked low hypodiploidy (mLH) by genomic duplication PMID: 26185311
  9. The allele T of rs10852936 surrounding the IKZF3-ZPBP2 locus confers risk for early-onset psoriasis. PMID: 25620289
  10. A strong association between rs907091 in the IKZF3 gene and SLE was identified. PMID: 25271777
  11. The immunosuppressive enzyme IL4I1 expressed differentially in human induced Aiolos+, but not natural Helios+, FOXP3+ Treg cells. PMID: 25446972
  12. A strong allelic association between SNPs in the 17q21 region (IKZF3) and asthma has been confirmed for the Latinos population. PMID: 24406073
  13. Overexpression of AIOLOS inhibits cell proliferation, suppresses apoptosis and arrests the cell cycle at the G0/G1 phase in vitro. PMID: 24399134
  14. Aiolos reconfigures chromatin structure within the SHC1 gene, causing isoform-specific silencing of the anchorage reporter p66(Shc) and blocking anoikis in vitro and in vivo. PMID: 24823637
  15. Sixteen SNPs were significantly associated with asthma of which one SNP was novel (IKZF3-rs1453559). PMID: 22626592
  16. Four additional susceptibility loci (IRF8, TMEM39A, IKZF3, and ZPBP2) for systemic lupus erythematosus were robustly established a multiethnic population (European, African American, Asian, Hispanic, Gullah, and Amerindian). PMID: 22464253
  17. To determine the consequences of Aiolos deregulation in B-CLL, we analyzed the effects of Aiolos overexpression or down-regulation on apoptosis. PMID: 21139082
  18. distribution of positive and negative expression of Aiolos and Helios found in various types of leukemias could implicate common pathways of their regulation PMID: 20432734
  19. Aiolos knockout mice develop the symptoms of human systemic lupus erythematosus (SLE). This finding implicates Aiolos as a possible candidate gene for SLE. PMID: 12574333
  20. we show for the first time the differential Aiolos expression at the RNA and protein level in hematopoietic cell subpopulations. PMID: 19540588
  21. Short isoforms of Aiolos were detected at low levels in bone marrow of normal volunters, but were not overexpressed in a patient with acute T-cell leukemia. Data for unaltered Aiolos indicates tumor suppressor function. PMID: 11937265
  22. impairment of Aiolos activity in human leukemia is not based on deficient phosphorylation as had been demonstrated in experiments in vitro PMID: 17296582
  23. this work demonstrates that the cellular activities of Aiolos isoforms are dependent on combinations of various functional domains arising from the differential splicing of mRNA transcripts PMID: 17646674
  24. up-regulation of Aiolos, confirmed at protein level, seems independent of Aiolos promoter H3K9 acetylation and H3K4 trimethylation PMID: 18184862
  25. DNA methylation directs Aiolos silencing and chromatin status in tumor cell lines, while in primary cells is mainly regulated by histone modifications. PMID: 18206652

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Subcellular Location Nucleus, Cytoplasm
Protein Families Ikaros C2H2-type zinc-finger protein family
Tissue Specificity Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus.
Database Links

HGNC: 13178

OMIM: 606221

KEGG: hsa:22806

STRING: 9606.ENSP00000344544

UniGene: Hs.371680

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