IKZF3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA552120A0HU
Size	US$210
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Image	IHC image of CSB-RA552120A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA552120A0HU diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Raji cells stained with CSB-RA552120A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q9UKT9

Target Names

IKZF3

Alternative Names

Zinc finger protein Aiolos (Ikaros family zinc finger protein 3), IKZF3, ZNFN1A3

Species Reactivity

Human

Immunogen

A synthesized peptide from human IKZF3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9E9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

IKZF3, also known as Aiolos, is a zinc finger transcription factor belonging to the Ikaros family that plays essential roles in lymphocyte development and immune regulation. This transcription factor is particularly critical for B cell maturation and differentiation, where it regulates genes involved in B cell receptor signaling and survival. IKZF3 has gained significant attention in cancer research due to its involvement in hematological malignancies, and it serves as a key target for immunomodulatory drugs used in multiple myeloma treatment, making it a valuable marker for both basic immunology and translational oncology studies.

This recombinant monoclonal antibody, generated from clone 9E9 in rabbit host, offers the reproducibility and consistency that demanding research protocols require. Because the antibody sequence is defined and produced recombinantly, you can expect uniform performance across experiments and over time, eliminating the lot-to-lot variability that can complicate long-term studies or multi-site collaborations. Affinity chromatography purification ensures high specificity for your target.

Validation testing demonstrates reliable performance across multiple applications. Immunohistochemistry studies in paraffin-embedded human tissues, including glioma and spleen sections, show clear detection using citrate buffer antigen retrieval at dilutions of 1:100. Flow cytometry analysis using Raji cells, a well-established B cell lymphoma line, confirms intracellular detection of IKZF3 with distinct signal separation from isotype controls. These validated applications provide flexibility for researchers investigating IKZF3 in both tissue-based and cell-based experimental systems.

Whether you are exploring lymphocyte biology, investigating therapeutic mechanisms of immunomodulatory compounds, or characterizing IKZF3 expression in malignancies, this antibody delivers the performance and consistency your research demands.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.

Gene References into Functions

The minor alleles of rs2941522, rs907091, rs1453559, rs12150079 and rs2872507 were statistically associated with an increased risk in Graves' disease patients. PMID: 29510406
Aiolos promotes the binding of Blimp-1 to target genes and thereby enhances Blimp-1-dependent transcriptional repression. PMID: 26823144
Our data indicate a possible regulatory role for the multiple sclerosis-associated IKZF3 and IQGAP1 variants. PMID: 27080863
results indicate that overexpression of Aiolos may contribute to pathogenesis of SLE and RA PMID: 26546109
Aiolos improved the survival of Nalm6 cells via PTEN and Aktdependent processes. PMID: 25608224
NFKB1, CD27, LAG3 and IKZF3 are new susceptibility genes for psoriasis. PMID: 25006012
lentivirus-mediated AIOLOS overexpression in Jurkat cells induced cell apoptosis, arrested the cell cycle at the G0/G1 phase, and synergistically increased the sensitivity of Jurkat cells to etoposide by inhibiting NF-kappaB activity. PMID: 25524659
identified a unique case of adult acute B-cell lymphoblastic leukemia with masked low hypodiploidy (mLH) by genomic duplication PMID: 26185311
The allele T of rs10852936 surrounding the IKZF3-ZPBP2 locus confers risk for early-onset psoriasis. PMID: 25620289
A strong association between rs907091 in the IKZF3 gene and SLE was identified. PMID: 25271777
The immunosuppressive enzyme IL4I1 expressed differentially in human induced Aiolos+, but not natural Helios+, FOXP3+ Treg cells. PMID: 25446972
A strong allelic association between SNPs in the 17q21 region (IKZF3) and asthma has been confirmed for the Latinos population. PMID: 24406073
Overexpression of AIOLOS inhibits cell proliferation, suppresses apoptosis and arrests the cell cycle at the G0/G1 phase in vitro. PMID: 24399134
Aiolos reconfigures chromatin structure within the SHC1 gene, causing isoform-specific silencing of the anchorage reporter p66(Shc) and blocking anoikis in vitro and in vivo. PMID: 24823637
Sixteen SNPs were significantly associated with asthma of which one SNP was novel (IKZF3-rs1453559). PMID: 22626592
Four additional susceptibility loci (IRF8, TMEM39A, IKZF3, and ZPBP2) for systemic lupus erythematosus were robustly established a multiethnic population (European, African American, Asian, Hispanic, Gullah, and Amerindian). PMID: 22464253
To determine the consequences of Aiolos deregulation in B-CLL, we analyzed the effects of Aiolos overexpression or down-regulation on apoptosis. PMID: 21139082
distribution of positive and negative expression of Aiolos and Helios found in various types of leukemias could implicate common pathways of their regulation PMID: 20432734
Aiolos knockout mice develop the symptoms of human systemic lupus erythematosus (SLE). This finding implicates Aiolos as a possible candidate gene for SLE. PMID: 12574333
we show for the first time the differential Aiolos expression at the RNA and protein level in hematopoietic cell subpopulations. PMID: 19540588
Short isoforms of Aiolos were detected at low levels in bone marrow of normal volunters, but were not overexpressed in a patient with acute T-cell leukemia. Data for unaltered Aiolos indicates tumor suppressor function. PMID: 11937265
impairment of Aiolos activity in human leukemia is not based on deficient phosphorylation as had been demonstrated in experiments in vitro PMID: 17296582
this work demonstrates that the cellular activities of Aiolos isoforms are dependent on combinations of various functional domains arising from the differential splicing of mRNA transcripts PMID: 17646674
up-regulation of Aiolos, confirmed at protein level, seems independent of Aiolos promoter H3K9 acetylation and H3K4 trimethylation PMID: 18184862
DNA methylation directs Aiolos silencing and chromatin status in tumor cell lines, while in primary cells is mainly regulated by histone modifications. PMID: 18206652

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

Ikaros C2H2-type zinc-finger protein family

Tissue Specificity

Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus.

Database Links

HGNC: 13178

OMIM: 606221

KEGG: hsa:22806

STRING: 9606.ENSP00000344544

UniGene: Hs.371680

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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Target Therapy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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