Human Zyxin(ZYX) ELISA kit

Instructions
Code CSB-EL027165HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name zyxin
Alternative Names ZYX ELISA Kit; Zyxin ELISA Kit; Zyxin-2 ELISA Kit
Abbreviation ZYX
Uniprot No. Q15942
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of Human ZYX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %88
Range %84-94
1:2Average %94
Range %90-98
1:4Average %92
Range %85-96
1:8Average %91
Range %88-95
Recovery
The recovery of Human ZYX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9389-97
EDTA plasma (n=4)8884-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15002.813 2.618 2.716 2.611
7502.318 2.414 2.366 2.261
3751.825 1.722 1.774 1.669
187.50.984 0.968 0.976 0.871
940.533 0.567 0.550 0.445
470.301 0.322 0.312 0.207
23.50.192 0.185 0.189 0.084
00.104 0.105 0.105
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Citations

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Target Data

Function Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).
Gene References into Functions
  1. Study findings identify zyxin as a physiological regulator of endothelial exocytosis through reorganizing local actin network in the final stage of exocytosis. Zyxin downregulation inhibits the secretion of VWF from human primary endothelial cells. PMID: 28256511
  2. these observations suggest that Zyxin promotes colon cancer tumorigenesis in a mitotic-phosphorylation-dependent manner and through CDK8-mediated YAP activation. PMID: 29967145
  3. Increased zyxin expression is associated with asthma. PMID: 28278518
  4. Zyxin binding to Ptc2 is due to the interaction of Zyxin 2nd LIM-domain (530-590 aa) with the under-membrane region of the cytoplasmic C-terminus of Ptc2 (1159-1412 aa). PMID: 27125030
  5. ZYX gene may be aging-related gene and involved in Osteoporosis. PMID: 25771989
  6. key regulator of stretch-induced gene expression at the cellular level PMID: 26071033
  7. Matrix density alters zyxin phosphorylation, which limits peripheral process formation and extension in endothelial cells invading 3D collagen matrices. PMID: 25038525
  8. Zyxin, which is thought to be essential for tight cell-to-cell junctions, decreased 1.8-fold in TNF-alpha-treated human brain microvascular endothelial cells. PMID: 25025691
  9. Integrin-beta5 and zyxin mediate formation of ventral stress fibers in response to transforming growth factor beta. PMID: 24036928
  10. Zyxin is a novel target for beta-amyloid peptide activities in Alzheimer's disease. PMID: 23330981
  11. Zyxin is suggested to promote growth, migration and invasiveness of fibroblastic type oral squamous cell carcinoma cells by upregulating Rac1 and Cdc42. PMID: 23292068
  12. A stretch-induced signaling pathway in vascular cells leads to the activation of zyxin, a cytoskeletal protein specifically involved in transducing mechanical stimuli. PMID: 23233529
  13. RA-induced cancer cell cytotoxicity was significantly impaired by Zyxin or PTOV1. Overall, our findings suggest that Zyxin and PTOV1 should be considered as critical determinants in cancer therapy with retinoids PMID: 23321499
  14. Zyxin is a transforming growth factor-beta (TGF-beta)/Smad3 target gene that regulates lung cancer cell motility via integrin alpha5beta1. PMID: 22778267
  15. Zyxin phenotype is mediated by partners alpha-actinin and p130Cas and ensures that motile cells in a three-dimensional matrix explore the largest space possible in minimum time. PMID: 22395610
  16. Depletion of nectin-2 from L cells expressing E-cadherin results in a loss of zyxin localization to cell-cell contacts, demonstrating that the zyxin-nectin interaction plays a critical role in zyxin targeting to these sites. PMID: 22056563
  17. LIM domain of zyxin recruits zyxin to force-bearing sites at the leading edge of migrating cells PMID: 21889443
  18. Findings suggest an unforeseen role for Zyxin in DNA damage-induced cell fate control through modulating the HIPK2-p53 signaling axis. PMID: 21248071
  19. Zyxin, whose expression is negatively regulated by microRNA-16 (miR-16), is confirmed to be a direct target gene of miR-16. Overexpression of zyxin could also restrain cell movement and enhance cell-cell adhesion. PMID: 21360639
  20. Stretch appears to be an important factor in the development of endothelial dysfunction with zyxin as a potential therapeutic target to interfere with these early changes in endothelial cell phenotype PMID: 20705921
  21. The LIM domain protein zyxin was identified as an interaction partner for human synemin. PMID: 19853601
  22. This study establishes a crucial role of zyxin in thrombin signaling in endothelial cells and provides evidence for a novel PAR-1 signaling pathway mediated by zyxin. PMID: 19690217
  23. LIM-nebulette, Lasp-1, and zyxin may play an important role in the organization of focal adhesions PMID: 15004028
  24. Within Ewing tumor cells, zyxin is expressed at very low levels and remains diffusely distributed throughout the cytoplasm, instead of concentrating in actin-rich dynamic structures. Zyxin has tumor suppressor activity in Ewing tumor cells. PMID: 15748890
  25. zyxin indirectly enables the interaction of ZNF384/CIZ/NMP4 with p130CAS PMID: 16510139
  26. A sarch for genes upregulated by thymosin beta4 identified zyxin in SiHA cells in the presence of exogenously added thymosin beta4 and when thymosin beta4 is overexpressed using adenoviral vectors. PMID: 16956606
  27. These results suggest that zyxin is involved in mechanical-force-dependent facilitation of actin polymerization at focal adhesions. PMID: 18682496
  28. We identified zyxin as an interactor with endoglin in human umbilical vein vascular endothelial cells and this interaction is localized within the 47-amino acid carboxyl-terminal cytosolic domain of endoglin, and maps within zyxin residues 326-572. PMID: 15084601

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Subcellular Location Cytoplasm, Cytoplasm, cytoskeleton, Nucleus, Cell junction, focal adhesion
Protein Families Zyxin/ajuba family
Database Links

HGNC: 13200

OMIM: 602002

KEGG: hsa:7791

STRING: 9606.ENSP00000324422

UniGene: Hs.490415

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