Human activated coagulation factor X(FXa) ELISA kit

Instructions
Code CSB-E12696h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name activated coagulation factor X(FXa)
Abbreviation FXa
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FXa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %92-100
1:2Average %92
Range %87-96
1:4Average %94
Range %90-98
1:8Average %89
Range %83-96
Recovery
The recovery of human FXa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9792-102
EDTA plasma (n=4)9690-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.536 2.598 2.567 2.405
101.735 1.768 1.752 1.590
51.036 1.098 1.067 0.905
2.50.650 0.698 0.674 0.512
1.250.446 0.432 0.439 0.277
0.6250.342 0.358 0.350 0.188
0.3120.240 0.261 0.251 0.089
00.161 0.163 0.162  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

Is it possible to work with citrate-plasma instead of EDTA- or heparin-plasma?
Can you please provide exemplary data from plasma samples?

A:
Thanks for your inquiry.. It is recommended to detect the EDTA plasma sample with the kit.
Generally pls detect the normal healthy human sample. Pls let me know if you have any further questions. Thank you.

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