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| Code | CSB-E09058h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<10% | ||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human AFP-L3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||||||||
| Sample | Serum(n=4) | |||||||||||
| 1:1 | Average % | 84 | ||||||||||
| Range % | 80-92 | |||||||||||
| 1:2 | Average % | 97 | ||||||||||
| Range % | 91-105 | |||||||||||
| 1:4 | Average % | 97 | ||||||||||
| Range % | 92-110 | |||||||||||
| 1:8 | Average % | 93 | ||||||||||
| Range % | 86-98 | |||||||||||
| The recovery of human AFP-L3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||||||||
| Sample Type | Average % Recovery | Range | ||||||||||
| Serum (n=5) | 95 | 89-98 | ||||||||||
| EDTA plasma (n=4) | 97 | 90-104 | ||||||||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Human AFP-L3 ELISA Kit was designed for the quantitative measurement of Human AFP-L3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.3 ng/mL.
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