Human amyloid beta peptide 1-40,Aβ1-40 ELISA Kit

Human amyloid beta peptide 1-40,Aβ1-40 ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

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Customer Reviews and Q&A

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Human amyloid beta peptide 1-40,Aβ1-40 ELISA Kit

Citations (11) Protocol MSDS

Code	CSB-E08299h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (11)
Related Products
Customer Reviews and Q&A

Target Name

amyloid beta peptide 1-40,Aβ1-40

Abbreviation

Aβ1-40

Species

Homo sapiens (Human)

Sample Types

serum, tissue homogenates, cerebrospinal fluid (CSF)

Detection Range

125 pg/mL-8000 pg/mL

Sensitivity

31.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Aβ1-40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	80-92
1:2	Average %	99
	Range %	91-105
1:4	Average %	95
	Range %	92-98
1:8	Average %	103
	Range %	96-110

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.846	2.687	2.767	2.610
4000	2.237	2.089	2.163	2.006
2000	1.679	1.612	1.646	1.489
1000	1.246	1.211	1.229	1.072
500	0.736	0.697	0.717	0.560
250	0.512	0.498	0.505	0.348
125	0.303	0.296	0.300	0.143
0	0.160	0.154	0.157

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human Aβ1-40 ELISA Kit was designed for the quantitative measurement of Human Aβ1-40 protein in serum, tissue homogenates, cerebrospinal fluid (CSF). It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.

GNG5 is a novel regulator of Aβ42 production in Alzheimer's disease C Li, Y Yang, S Luo, W Qiu, X Wang, W Ge,Cell Death & Disease,2024
Oab-14 Alleviates Mitochondrial Impairment Through the Sirt3-Dependent Mechanism in App/Ps1 Transgenic Mice and N2a/App Cells N Zheng, R Cao, D Liu, P Liu, X Zhao, S Zhang,Available at SSRN 5013649,/
Targeting blood brain barrier—Remote ischemic conditioning alleviates cognitive impairment in female APP/PS1 rats Y Ma,CNS neuroscience & therapeutics,2024
The aldehyde dehydrogenase 2 rs671 variant enhances amyloid β pathology X Wang,Nature communications,2024
Melatonin alleviates depression-like behaviors and cognitive dysfunction in mice by regulating the circadian rhythm of AQP4 polarization D Yao,Translational psychiatry,2023
Extracellular Vesicles Play a Central Role in Cerebral Venous Disease‐Associated Brain Atrophy JY Wang,Advanced science,2023
Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model M Hu,Nature communications,2023
Magnolol improves Alzheimer's disease-like pathologies and cognitive decline by promoting autophagy through activation of the AMPK/mTOR/ULK1 pathway X Wang,Biomedicine & pharmacotherapy,2023
Activating AhR alleviates cognitive deficits of Alzheimer's disease model mice by upregulating endogenous Aβ catabolic enzyme Neprilysin C Qian,Research Square,2021
Notopterygium incisum extract (NRE) rescues cognitive deficits in APP/PS1 Alzhneimer's disease mice by attenuating amyloid-beta, tau, and neuroinflammation pathology Jiang X, et al,Journal of Ethnopharmacology,2019
Induced dural lymphangiogenesis facilities soluble amyloid-beta clearance from brain in a transgenic mouse model of Alzheimer's disease Ya-Ru Wen.et al,NRR,2018

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■ Q&A

We are interested in this kit.Could you let me know the information about the following ?
Do you have the information of capture antibody coated on the plate and biotinylated detection antibody in the kit?

Thanks for your inquiry.
Capture antibody:mouse monoclonal antibody;Detection antibody:goat polyclonal antibody.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.