| Description |
This Human Surv Ab ELISA Kit was designed for the quantitative measurement of Human Surv Ab protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL. |
| Target Name |
anti-Survivin(Surv) antibody |
| Abbreviation |
Surv Ab |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma |
| Detection Range |
0.625 ng/mL-40 ng/mL |
| Sensitivity |
0.156 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human anti-surv antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
85 |
|
| Range % |
81-90 |
|
| 1:2 |
Average % |
96 |
|
| Range % |
90-105 |
|
| 1:4 |
Average % |
86 |
|
| Range % |
81-90 |
|
| 1:8 |
Average % |
93 |
|
| Range % |
85-100 |
|
|
| Recovery |
| The recovery of human anti-surv antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
90-101 |
|
| EDTA plasma (n=4) |
87 |
82-92 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 40 |
2.213 |
2.157 |
2.185 |
2.014 |
|
| 20 |
1.612 |
1.512 |
1.562 |
1.391 |
|
| 10 |
1.156 |
1.113 |
1.135 |
0.964 |
|
| 5 |
0.779 |
0.735 |
0.757 |
0.586 |
|
| 2.5 |
0.522 |
0.492 |
0.507 |
0.336 |
|
| 1.25 |
0.383 |
0.366 |
0.375 |
0.204 |
|
| 0.625 |
0.267 |
0.283 |
0.275 |
0.104 |
|
| 0 |
0.168 |
0.173 |
0.171 |
|
|
|
|
| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
