Human anti-Survivin(Surv) antibody ELISA Kit

Code CSB-E11874h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Human Surv Ab ELISA Kit was designed for the quantitative measurement of Human Surv Ab protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

Target Name anti-Survivin(Surv) antibody
Abbreviation Surv Ab
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human anti-surv antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 81-90  
1:2 Average % 96  
Range % 90-105  
1:4 Average % 86  
Range % 81-90  
1:8 Average % 93  
Range % 85-100  
The recovery of human anti-surv antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-101  
EDTA plasma (n=4) 87 82-92  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
40 2.213 2.157 2.185 2.014  
20 1.612 1.512 1.562 1.391  
10 1.156 1.113 1.135 0.964  
5 0.779 0.735 0.757 0.586  
2.5 0.522 0.492 0.507 0.336  
1.25 0.383 0.366 0.375 0.204  
0.625 0.267 0.283 0.275 0.104  
0 0.168 0.173 0.171    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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