Human apelin 13,AP13 ELISA Kit

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Human apelin 13,AP13 ELISA Kit

Citations (1) Protocol MSDS

Code	CSB-E13072h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (1)
Customer Reviews and Q&A

Target Name

apelin 13,AP13

Abbreviation

AP13

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

6.25 pg/mL-400 pg/mL

Sensitivity

1.56 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human AP13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	104
	Range %	100-108
1:2	Average %	98
	Range %	91-105
1:4	Average %	101
	Range %	94-108
1:8	Average %	94
	Range %	89-100

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

400

2.354

2.434

2.394

2.316

200

1.326

1.337

1.332

1.253

100

0.798

0.818

0.808

0.730

0.502

0.529

0.516

0.437

0.249

0.251

0.250

0.172

12.5

0.147

0.153

0.150

0.072

6.25

0.123

0.118

0.121

0.042

0.076

0.081

0.078

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human AP13 ELISA Kit was designed for the quantitative measurement of Human AP13 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

Apelin modulates inflammation and leukocyte recruitment in experimental autoimmune encephalomyelitis H Park,Nature communications,2024

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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