Code | CSB-E08100h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:200 | Average % | 96 | ||||
Range % | 92-102 | |||||
1:400 | Average % | 95 | ||||
Range % | 91-99 | |||||
1:800 | Average % | 104 | ||||
Range % | 102-108 | |||||
1:1600 | Average % | 87 | ||||
Range % | 81-92 |
The recovery of human Apo-B100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 92-102 | ||||
EDTA plasma (n=4) | 90 | 87-94 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human APOB100 ELISA Kit is intended to measure human APOB100 in serum, plasma, cell culture supernates, saliva, or urine. This assay employs the competitive inhibition enzyme immunoassay technique and enzyme-substrate chromogenic reaction to quantify human APOB100 concentration. The intensity of the colored product is negatively proportional to the concentration of APOB100 present in the sample. This human APOB100 ELISA kit has high specificity and excellent sensitivity.
APOB100 is synthesized in the liver in human that is required for normal removal and metabolism of low-density lipoproteins (LDL). It is the core protein of the LDL and controls its internalization by binding to the LDL receptor. It is an autoantigen that drives the production of pathogenic Th1 cells with proinflammatory cytokine secretion. Mutations in APOB100 can cause familial hypercholesterolemia. APOB100 has a central role in the development of atherosclerosis. Through the recognition of APOB100 by macrophages, LDL accumulates in plaques within the arterial walls, inducing a prolonged activation of inflammatory responses and resulting in enlargement and weakening of the plaques.
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