Human apolipoprotein B100 (Apo-B100) ELISA Kit

Code CSB-E08100h
Size 96T,5×96T,10×96T
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Product Details

Target Name
apolipoprotein B (including Ag(x) antigen)
Alternative Names
Apo B 100 ELISA Kit; Apo B ELISA Kit; Apo B-100 ELISA Kit; Apo B-48 ELISA Kit; Apo B100 ELISA Kit; Apo B48 ELISA Kit; ApoB 100 ELISA Kit; ApoB 48 ELISA Kit; APOB ELISA Kit; APOB_HUMAN ELISA Kit; Apolipoprotein B (including Ag(x) antigen) ELISA Kit; Apolipoprotein B 100 ELISA Kit; Apolipoprotein B 48 ELISA Kit; Apolipoprotein B ELISA Kit; Apolipoprotein B-48 ELISA Kit; Apolipoprotein B100 ELISA Kit; Apolipoprotein B48 ELISA Kit; FLDB ELISA Kit; LDLCQ4 ELISA Kit
Abbreviation
APOB
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, saliva, urine
Detection Range
78 ng/mL-5000 ng/mL
Sensitivity
19.5 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:200Average %96
Range %92-102
1:400Average %95
Range %91-99
1:800Average %104
Range %102-108
1:1600Average %87
Range %81-92
Recovery
The recovery of human Apo-B100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9592-102
EDTA plasma (n=4)9087-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
50000.074 0.073 0.074
25000.118 0.121 0.120
12500.182 0.172 0.177
6250.272 0.284 0.278
3120.451 0.442 0.447
1560.770 0.754 0.762
781.122 1.054 1.088
02.371 2.270 2.321
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The human APOB100 ELISA Kit is intended to measure human APOB100 in serum, plasma, cell culture supernates, saliva, or urine. This assay employs the competitive inhibition enzyme immunoassay technique and enzyme-substrate chromogenic reaction to quantify human APOB100 concentration. The intensity of the colored product is negatively proportional to the concentration of APOB100 present in the sample. This human APOB100 ELISA kit has high specificity and excellent sensitivity.

APOB100 is synthesized in the liver in human that is required for normal removal and metabolism of low-density lipoproteins (LDL). It is the core protein of the LDL and controls its internalization by binding to the LDL receptor. It is an autoantigen that drives the production of pathogenic Th1 cells with proinflammatory cytokine secretion. Mutations in APOB100 can cause familial hypercholesterolemia. APOB100 has a central role in the development of atherosclerosis. Through the recognition of APOB100 by macrophages, LDL accumulates in plaques within the arterial walls, inducing a prolonged activation of inflammatory responses and resulting in enlargement and weakening of the plaques.

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 Q&A
Q:

Can you provide us with Human ApoB48 elisa kits?

A:
Thanks for your inquiry.
CSB-E08100h Human apolipoprotein B100 (Apo-B100) ELISA Kit is the proper kit for you. I will ask our distributor in your area to give you a quote.

Target Background

Function
(From Uniprot)
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Gene References into Functions
  1. Authors performed an analysis of public databases and literature for every variant published associated with FH, in the genes LDLR, APOB, and PCSK9. PMID: 29261184
  2. Targeted next generation DNA sequencing revealed several rare heterozygous missense variants in both MTTP and APOB genes known or predicted to be deleterious, in addition to a novel heterozygous missense variant in SAR1B, which encodes the gene causing chylomicron retention disease PMID: 29540175
  3. Single nucleotide polymorphisms rs693 and rs6725189 of the apoB gene are associated with calcific aortic stenosis in Chinese subjects, in Xinjiang, China. PMID: 29514644
  4. In conclusion, this study demonstrated that an APOB SNP, rs1042034, is closely associated with HCV infection through lipid metabolism alteration. PMID: 29382324
  5. APOB rs11279109 is associated with increased risk of coronary heart disease in Kuwaiti population. PMID: 29362515
  6. The simultaneous retention of fibrinogen and APOB-lipoproteins in FSD can be detected in routinely stained histological sections. The analysis of protein structures unraveled the pathomorphogenesis of this unexpected phenomenon. Fibrinogen gamma chain mutations provoke conformational changes in the region of the globular domain involved in the "end-to-end" interaction, thus impairing the D-dimer formation PMID: 29244742
  7. APOB polymorphism rs679899 is associated with type 2 diabetes and glutamyl transpeptidase levels, while the LIPC polymorphism rs6083 may influence plasma lipid levels in Chinese Han population. PMID: 29883758
  8. We present a case of homozygous familial defective apolipoprotein B-100 due to APOB R3500Q (rs5742904) treated with evolocumab ..Identification of a patient homozygous for familial defective apolipoprotein B-100(FDB) and successful treatment with PCSK9 inhibition PMID: 28988723
  9. Increased LDL levels were observed over time in patients with the -7673G>A polymorphism of the ApoB gene. PMID: 29846435
  10. Review/Meta-analysis: significant association of APOB rs1801701 GA, and APOB rs1042031 GA with increased risk of ischemic stroke. PMID: 28865324
  11. Serum apoB/A-I ratio appears to have value for predicting SAP in patients with AP. PMID: 28677336
  12. Apolipoprotein B is associated with carotid atherosclerosis progression PMID: 28826575
  13. Results show differences in the apoB100 secondary structure content among lipoproteins incubated at pH 7.4 or at pH 5.0 that could resemble those occurring in the early or late endosomes, respectively. PMID: 27824107
  14. Elevated serum apoB levels independently predict an increased risk for incident non-alcoholic fatty liver disease. PMID: 28106941
  15. This study shows that the ApoB-516C/T promoter gene polymorphism has no impact on the risk of hepatitis C virus infection. However, the C/T genotype may be a protective factor for females. PMID: 28370191
  16. association of TM6SF2 rs58542926 genotype with increased serum tyrosine levels and decreased apoB-100 particles in Finns PMID: 28539357
  17. that apos involved in TG metabolism such as apoC2, C3, E, and A4 (micromolar concentration), and apoB48 and apoA5 (single-digit nanomolar concentration) can be quantified from a single digestion mixture. PMID: 24694356
  18. These findings indicate that maternal apo B levels are significantly associated with apo B levels in their pre-school age children, adjusted for confounding variables. Furthermore, the mother-child correlations in apo B levels were independent of mother-child adiposity. Measurement of apo B levels in mothers may identify both high-risk children and mothers who may benefit from intervention. PMID: 28799493
  19. Individuals with apoB higher than predicted by non-HDL-C had significantly higher levels of PAI-1, which may contribute to the increased risk of future atherothrombotic events PMID: 28502501
  20. analysis of five likely pathogenic heterozygous rare variants that include four novel nonsense mutations in APOB (p.Gln845*, p.Gln2571*, p.Cys2933* and p.Ser3718*) and a rare variant in PCSK9 (Minor Allele Frequency <0.1%). PMID: 27179706
  21. In this sample of older adults with mild cognitive impairment, the presence of at least one copy of ApoE4 was associated with the development of both increased gait variability and cognitive decline during 1 year of follow-up PMID: 28482102
  22. The impact of smoking on the levels of apolipoprotein B (APOB) was evaluated by analyzing data from NHANES for the years 2007-2012 for US adolescents aged 12-19 years and adults aged greater than or eqult to 20 years. The study found that smoking did not influence the observed levels of APOB for either adolescents or adults. PMID: 28672200
  23. Identify LDLR, APOB and PCSK9 novel mutations causing familial hypercholesterolemia in the central south region of China. PMID: 28235710
  24. Rare variants of APOB or PCSK9 were identified in nine of the 22 study patients with extremely low LDL-C levels PMID: 29036232
  25. Eicosapentaenoic acid has direct antioxidant benefits in various apoB-containing subfractions. PMID: 26945158
  26. Association of plasma apoB with IR in obese subjects is dependent on gynoid WAT dysfunction PMID: 28391908
  27. Serum ApoB was not significantly different between term SGA newborns and control term newborns. PMID: 28304324
  28. APOB associated with Familial Hypercholesterolemia and Polygenic Hypercholesterolemia in patients with Acute Coronary Syndrome , age /=160 mg/dl. PMID: 28958330
  29. we identified common intronic SNPs (rs676210 and rs1042034) in the APOB gene and found five SNPs that were collectively associated with hyperlipidemia in the adult Chinese Yugur population. Importantly, we demonstrated that the G allele of rs676210 may confer an increased risk of hyperlipidemia. PMID: 28902930
  30. APOB missense variants, A224T and V925L, in a black South African woman with marked hypocholesterolemia PMID: 27206948
  31. male gender, ageing in women and menopause were associated with increased apoB concentrations. PMID: 27086565
  32. Data provide no evidence supporting an association between the APOB R3527Q variant and type 2 diabetes or glycemia. PMID: 28428224
  33. Levels of lipoprotein (a) [Lp(a)], a complex between an LDL-like lipid moiety containing one copy of apoB, and apo(a), a plasminogen-derived carbohydrate-rich hydrophilic protein, are primarily genetically rIncreasing evidence suggests that age, sex, and hormonal impact may have a modest modulatory influence on Lp(a) levels. Among clinical conditions, Lp(a) levels are reported to be affected by kidney and liver diseases. PMID: 26637279
  34. Data suggest that amphipathic beta-strands in 200 N-terminal residues of beta1 domain of APOB are required for secretion of lipid-rich or lipid-poor particles; residues 300-700 or 1050-1500 of beta1 domain appear to be required for secretion of lipid-rich particles; MTTP is required for secretion of intact APOB but not of truncated APOB. (APOB = apolipoprotein B; MTTP = microsomal triglyceride transfer protein) PMID: 28702990
  35. Basal apoB secretion by BeWo cells supports the concept of basal lipoprotein particle secretion by placental syncytiotrophoblast. PMID: 28487135
  36. ApoB EcoRI confers a moderate risk for coronary heart disease and the E(-) allele at this locus might be a susceptibility allele for the development of disease (Meta-Analysis) PMID: 27637205
  37. APOB single nucleotide polymorphism and it's association with LDL cholesterol level and risk of coronary heart disease in Pakistani population PMID: 27112212
  38. High apoB48 concentration is associated with Atherosclerotic Lesion Progression. PMID: 27487947
  39. Autoantibodies to oxidized lipoproteins and immune complexes with apoB-100 lipoproteins vary significantly by sex, age, and ethnicity. Higher baseline IgG MDA-LDL titers independently associate with new major adverse cardiovascular events. PMID: 28473443
  40. miR-548p regulates apoB secretion by targeting its mRNA degradation. PMID: 28336556
  41. Adolescent girls with obesity and polycystic ovary syndrome have elevated fasting and postprandial plasma triglycerides and ApoB-lipoprotein remnants. PMID: 27997268
  42. the associations of rs693 and rs562338 polymorphisms representing the Apolipoprotein B locus with endophenotypes (total cholesterol [TC] and high-density lipoprotein cholesterol) and phenotypes, were examined. PMID: 27683205
  43. ApoB100 is indispensable for HCV infection. ApoB is required for the generation of fully infectious HCV virions. PMID: 28018102
  44. a higher inflammatory status is associated with decreased triglyceride-rich-rich lipoprotein apoB-48 secretion among insulin resistant subjects PMID: 28183448
  45. Several protein markers were also deregulated in extracellular vesicless from bladder tumour patients, and our data suggest that the presence of ApoB in the 100,000 pellet is a clear marker for malignancy. PMID: 27751843
  46. 10 association studies for Han Chinese, with 1195 CHD patients and 1178 healthy subjects, we found that XbaI (rs693) polymorphisms in APOB were statistically significantly associated with Coronary Heart Disease in Han Chinese population PMID: 27172140
  47. We observed a significant interaction between Ins/Del ApoB genotype and dietary omega-3 PUFA intake with respect to BMI, WC, and obesity risk in both unadjusted (P = 0.007, P = 0.001, and P = 0.021, respectively) and adjusted (P = 0.007, P = 0.04, and P = 0.002, respectively) samples in NIDDM patients. PMID: 27210509
  48. Multiple novel LDLR and ApoB mutations have been identified in a-United Kingdom-based cohort with familial hypercholesterolemia. PMID: 26748104
  49. Data suggest that LDL-C/apoB ratio (ratio of plasma level of total cholesterol to plasma level of apolipoprotein B) is an important biomarker in overall evaluation of atherogenicity in obese, overweight, and normal-weight men; this study of qualitative changes in lipid/lipoprotein profile for early diagnosis of dyslipidemias leading to atherosclerosis was conducted in Russia. PMID: 27347637
  50. Mutations in APOB is associated with hypobetalipoproteinemia. PMID: 26916057

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Involvement in disease
Hypobetalipoproteinemia, familial, 1 (FHBL1); Familial ligand-defective apolipoprotein B-100 (FDB)
Subcellular Location
Cytoplasm. Secreted. Lipid droplet.
Database Links

HGNC: 603

OMIM: 107730

KEGG: hsa:338

STRING: 9606.ENSP00000233242

UniGene: Hs.120759

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