Recombinant Human Apolipoprotein B-100(APOB),partial

In Stock
Code CSB-EP001918HUb0
Size US$1726

Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names APOB
Uniprot No. P04114
Research Area Epigenetics and Nuclear Signaling
Species Homo sapiens (Human)
Source E.coli
Expression Region 28-127aa
Target Protein Sequence EEEMLENVSLVCPKDATRFKHLRKYTYNYEAESSSGVPGTADSRSATRINCKVELEVPQLCSFILKTSQCTLKEVYGFNPEGKALLKKTKNSEEFAAAMS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 14.7 kDa
Protein Length Partial
Tag Info N-terminal 10xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA Please contact us to get it.
Still Have Questions? Leave a Message or Start an on-line Chat

Q&A and Customer Reviews

 Q&A
Q:

I'm looking for the full-length human recombinant ApoB-48 protein?

A:
Thanks for your inquiry.
Reference: https://www.uniprot.org/uniprot/P04114
The full-length human recombinant ApoB-48 protein refers to the region of 28-2179aa, the length is 2152aa, which is too difficult to express, sorry, we can't provide.Do you accept the partial protein ? If yes, please tell us the fragment of interest, we will check if we can provide.
Q:

Thank you for your response, I'm wondering what the maximum length of the ApoB-48 protein you can produce? I'm looking for a protein that is as complete as possible.

A:
Thanks for your inquiry.
Generally, The longer the length, the more difficult it is to express. The length is 2152aa, I'm afraid the full length protein is impossible to express.
For the time being, we can offer risk-free custom service for expressing protein within 800aa,and we can try to express longer protein within 1200aa,
but the custom service for protein expression with length over 800aa is charged step by step.
1.Protein length within 800aa: Risk-free custom service
2.Protein length over 800aa but less than 1200aa: Custom service charged step by step.
3.Protein length over 1200aa: We would rather not to try, but if the customer insists, we can try to express, it's charged step by step.
In addition, could you pls tell us your application of this protein ? Do it must be the purified protein, is the lysate be OK ?
If the lysate is OK, the success rate would be much higher.
BTW, we need to choose the suitable expression region according to your application, or if you could tell us the region, that would be better.
We can try any region with the length less than 1200aa.
Previously, we have successfully developed a partial protein listed as below:
Recombinant Human Apolipoprotein B-100(APOB),partial
CSB-EP001918HUb0 >> E.coli
Expression Region: 28-127aa; Partial.
Tag information:N-terminal 10xHis-tagged.
Sequence:

EEEMLENVSLVCPKDATRFKHLRKYTYNYEAESSSGVPGTADSRSATRINCKVELEVPQLCSFILKTSQCTLKEVYGFNPEGKALLKKTKNSEEFAAAMS

 Customer Reviews

Target Data

Function Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Involvement in disease Hypobetalipoproteinemia, familial, 1 (FHBL1); Familial ligand-defective apolipoprotein B-100 (FDB)
Subcellular Location Cytoplasm, Secreted
Database Links

HGNC: 603

OMIM: 107730

KEGG: hsa:338

STRING: 9606.ENSP00000233242

UniGene: Hs.120759

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.