Human blca-4 ELISA Kit

Code CSB-E14959h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
blca-4
Abbreviation
BLCA-4
Species
Homo sapiens (Human)
Sample Types
serum, plasma, urine, tissue homogenates
Detection Range
0.625 ng/mL-40 ng/mL
Sensitivity
0.156 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human blca-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 84-91  
1:2 Average % 99  
Range % 96-103  
1:4 Average % 104  
Range % 101-107  
1:8 Average % 95  
Range % 89-99  
Recovery
The recovery of human blca-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 87 84-90  
EDTA plasma (n=4) 92 88-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
40 2.595 2.540 2.568 2.452  
20 2.208 2.365 2.287 2.171  
10 1.823 1.804 1.814 1.698  
5 1.195 1.175 1.185 1.069  
2.5 0.698 0.678 0.688 0.572  
1.25 0.418 0.432 0.425 0.309  
0.625 0.271 0.276 0.274 0.158  
0 0.115 0.117 0.116    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human BLCA-4 ELISA Kit was designed for the quantitative measurement of Human BLCA-4 protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

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 Q&A
Q:

I am interested in purchasing the kit for a study that we wanted to perform. Can you please tell me more abou the antibodies that comprise this kit?

A:
Thanks for your inquiry.
CSB-E14959h The kit uses double antibody sandwich method to quatitative detect blca-4.
And its coated antibody is goat polyclonal antibody, biotin-conjugated antibody (that is, detection antibody) is goat polyclonal antibody
As for the specific components of the kit can be seen in the manual MATERIALS PROVIDED part.I will send the manual to you by e-mail. Pls let me know if you have any further questions. Thank you.
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