Human carbonhydrate antigen 19-9 (CA19-9) ELISA kit

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Code CSB-E04773h
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
carbonhydrate antigen 19-9 (CA19-9)
Alternative Names
CA199
Abbreviation
CA19-9
Species
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
10 U/mL-150 U/mL
Sensitivity
3.8 U/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<20%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CA19-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Sample

Serum(n=4)

1:1

Average %

96

Range %

90-110

1:2

Average %

89

Range %

85-99

1:4

Average %

93

Range %

87-101

1:8

Average %

95

Range %

89-98

Recovery

The recovery of human CA19-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

97

93-100

EDTA plasma (n=4)

97

90-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

U/ml

OD1

OD2

Average

 

150

2.045

2.011

2.028

 

100

1.292

1.300

1.296

 

40

0.728

0.735

0.732

 

10

0.304

0.306

0.305

 

Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Human CA19-9 ELISA Kit was designed for the quantitative measurement of Human CA19-9 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 10 U/mL-150 U/mL and the sensitivity is 3.8 U/mL.

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