Human cholecystokinin octapeptide,CCK-8 ELISA Kit

Instructions
Code CSB-E08986h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name cholecystokinin octapeptide,CCK-8
Abbreviation CCK-8
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 0.57 ng/mL-10 ng/mL
Sensitivity 0.357 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CCK-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %85-97
1:2Average %96
Range %90-106
1:4Average %95
Range %86-104
1:8Average %93
Range %88-96
Recovery
The recovery of human CCK-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9893-108
EDTA plasma (n=4)9589-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
0.570.048 0.051 0.050
1.140.162 0.153 0.158
2.290.376 0.363 0.370
5.710.838 0.842 0.840
101.655 1.667 1.661
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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