| Description |
This Human CCK-8 ELISA Kit was designed for the quantitative measurement of Human CCK-8 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.57 ng/mL-10 ng/mL and the sensitivity is 0.357 ng/mL . |
| Target Name |
cholecystokinin octapeptide,CCK-8 |
| Abbreviation |
CCK-8 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma |
| Detection Range |
0.57 ng/mL-10 ng/mL |
| Sensitivity |
0.357 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<15% | | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | | Inter-assay Precision (Precision between assays): CV%<15% | | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | | |
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human CCK-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | | Sample | Serum(n=4) | | | 1:1 | Average % | 92 | | | Range % | 85-97 | | | 1:2 | Average % | 96 | | | Range % | 90-106 | | | 1:4 | Average % | 95 | | | Range % | 86-104 | | | 1:8 | Average % | 93 | | | Range % | 88-96 | |
|
| Recovery |
| The recovery of human CCK-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range | | | Serum (n=5) | 98 | 93-108 | | | EDTA plasma (n=4) | 95 | 89-103 | | | | | | | | | | | | | | | | | |
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
 | ng/ml | OD1 | OD2 | Average | | | | 0.57 | 0.048 | 0.051 | 0.050 | | | | 1.14 | 0.162 | 0.153 | 0.158 | | | | 2.29 | 0.376 | 0.363 | 0.370 | | | | 5.71 | 0.838 | 0.842 | 0.840 | | | | 10 | 1.655 | 1.667 | 1.661 | | |
|
|
| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
