Human cholecystokinin octapeptide,CCK-8 ELISA Kit

Code CSB-E08986h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details

Target Name
cholecystokinin octapeptide,CCK-8
Abbreviation
CCK-8
Species
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
0.57 ng/mL-10 ng/mL
Sensitivity
0.357 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CCK-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %85-97
1:2Average %96
Range %90-106
1:4Average %95
Range %86-104
1:8Average %93
Range %88-96
Recovery
The recovery of human CCK-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9893-108
EDTA plasma (n=4)9589-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
0.570.048 0.051 0.050
1.140.162 0.153 0.158
2.290.376 0.363 0.370
5.710.838 0.842 0.840
101.655 1.667 1.661
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human CCK-8 ELISA Kit was designed for the quantitative measurement of Human CCK-8 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.57 ng/mL-10 ng/mL and the sensitivity is 0.357 ng/mL .

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