Human cleaved microtubule-associated protein tau (C-MAPT/C-TAU) ELISA kit

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Human cleaved microtubule-associated protein tau (C-MAPT/C-TAU) ELISA kit

Citations (1) Protocol MSDS

Code	CSB-ECL013481H
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (1)
Customer Reviews and Q&A

Target Name

cleaved microtubule-associated protein tau

Alternative Names

cleaved form of DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU, G protein beta1/gamma2 subunit-interacting factor 1|microtubule-associated protein tau, isoform 4

Abbreviation

C-MAPT/C-TAU

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cerebrospinal fluid (CSF), tissue homogenates, cell lysates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human C-MAPT/C-TAU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	102
	Range %	96-106
1:10	Average %	100
	Range %	90-104
1:20	Average %	95
	Range %	85-99
1:40	Average %	94
	Range %	88-99

Recovery

The recovery of human C-MAPT/C-TAU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-95
EDTA plasma (n=4)	100	91-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	3.001	2.889	2.945	2.832
2000	2.114	1.976	2.045	1.932
1000	1.231	1.200	1.216	1.103
500	0.596	0.565	0.581	0.468
250	0.353	0.341	0.347	0.234
125	0.239	0.232	0.236	0.123
62.5	0.171	0.168	0.170	0.057
0	0.111	0.115	0.113

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human C-MAPT/C-TAU ELISA Kit was designed for the quantitative measurement of Human C-MAPT/C-TAU protein in serum, plasma, cerebrospinal fluid (CSF), tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

A prospective pilot study on serum cleaved tau protein as a neurologicalMarker in severe traumatic brain injury Pandey S.et al,Br J Neurosurg,2017

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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