Human cross linked N-telopeptide of type Ⅰ collagen,NTX ELISA Kit

Human cross linked N-telopeptide of type Ⅰ collagen,NTX ELISA Kit

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Human cross linked N-telopeptide of type Ⅰ collagen,NTX ELISA Kit

Name: Human cross linked N-telopeptide of type Ⅰ collagen,NTX ELISA Kit
Brand: CUSABIO
SKU: CSB-E09233h

^Instructions

Code	CSB-E09233h
Size	96T,5×96T,10×96T
Trial Size
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Target Name

cross linked N-telopeptide of type Ⅰ collagen,NTX

Alternative Names

cross linked N-telopeptide of type 1 collagen,NTX,cross linked N-telopeptide of type I collagen,NTX

Abbreviation

NTX

Species

Homo sapiens (Human)

Sample Types

serum, plasma, urine

Detection Range

7.8 nM BCE-500 nM BCE

Sensitivity

1.95 nM BCE

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human NTX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	98
	Range %	94-102
1:4	Average %	102
	Range %	98-106
1:8	Average %	95
	Range %	92-99

Recovery

The recovery of human NTX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-106
EDTA plasma (n=4)	92	88-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

nM BCE	OD1	OD2	Average	Corrected
500	1.983	1.976	1.980	1.850
250	1.446	1.550	1.498	1.368
125	1.011	1.093	1.052	0.922
62.5	0.616	0.625	0.621	0.491
31.25	0.374	0.361	0.368	0.238
15.6	0.272	0.274	0.273	0.143
7.8	0.204	0.215	0.210	0.080
0	0.131	0.129	0.130

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Efficacy and Safety of Kudzu Flower–Mandarin Peel on Hot Flashes and Bone Markers in Women during the Menopausal Transition: A Randomized Controlled Trial JE Kim,Nutrients,2020
Effects of short-term aerobic exercise with and without external loading on bone metabolism and balance in postmenopausal women with osteoporosis /,Rheumatology International,2013
Smad, but not MAPK, pathway mediates the expression of type I collagen in radiation induced fibrosis /,Biochemical and Biophysical Research Communications,2012

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■ Q&A

Can you run this assay on samples (whole blood or serum) that has been previously frozen or do samples have to be fresh?

Thanks for your inquiry.
CSB-E09233h
This kit can test serum and plasma samples( not whole blood). It's better to test fresh samples；
If the sample has been previously frozen, pls pay attention to the storage time and avoid repeat freezing and thawing. Repeat freezing and thawing will destroy the structure of protein and affects detection. We suggest you do a pretest first if you want to test frozen sample.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.