Human cross linked N-telopeptide of type Ⅰ collagen,NTX ELISA Kit

Instructions
Code CSB-E09233h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cross linked N-telopeptide of type Ⅰ collagen,NTX
Alternative Names cross linked N-telopeptide of type 1 collagen,NTX,cross linked N-telopeptide of type I collagen,NTX
Abbreviation NTX
Species Homo sapiens (Human)
Sample Types serum, plasma, urine
Detection Range 7.8 nM BCE-500 nM BCE
Sensitivity 1.95 nM BCE
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human NTX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 82-93  
1:2 Average % 98  
Range % 94-102  
1:4 Average % 102  
Range % 98-106  
1:8 Average % 95  
Range % 92-99  
Recovery
The recovery of human NTX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 85-106  
EDTA plasma (n=4) 92 88-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
nM BCE OD1 OD2 Average Corrected  
500 1.983 1.976 1.980 1.850  
250 1.446 1.550 1.498 1.368  
125 1.011 1.093 1.052 0.922  
62.5 0.616 0.625 0.621 0.491  
31.25 0.374 0.361 0.368 0.238  
15.6 0.272 0.274 0.273 0.143  
7.8 0.204 0.215 0.210 0.080  
0 0.131 0.129 0.130    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

Can you run this assay on samples (whole blood or serum) that has been previously frozen or do samples have to be fresh?

A:
Thanks for your inquiry.
CSB-E09233h
This kit can test serum and plasma samples( not whole blood). It's better to test fresh samples;
If the sample has been previously frozen, pls pay attention to the storage time and avoid repeat freezing and thawing. Repeat freezing and thawing will destroy the structure of protein and affects detection. We suggest you do a pretest first if you want to test frozen sample.

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