Human cytokeratin fragment antigen 21-1,CYFRA21-1 ELISA Kit

Code CSB-E09606h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human CYFRA21-1 ELISA Kit was designed for the quantitative measurement of Human CYFRA21-1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

Target Name cytokeratin fragment antigen 21-1,CYFRA21-1
Abbreviation CYFRA21-1
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CYFRA21-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 82-90  
1:2 Average % 98  
Range % 91-105  
1:4 Average % 99  
Range % 94-110  
1:8 Average % 92  
Range % 86-99  
Recovery
The recovery of human CYFRA21-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 80-98  
EDTA plasma (n=4) 97 90-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.541 2.385 2.463 2.305  
10 2.229 2.082 2.156 1.998  
5 1.607 1.545 1.576 1.418  
2.5 0.866 0.842 0.854 0.696  
1.25 0.463 0.439 0.451 0.293  
0.625 0.337 0.324 0.331 0.173  
0.312 0.254 0.246 0.250 0.092  
0 0.160 0.156 0.158    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

May I know are the antibodies used in the ELISA kit polyclonal antibodies or monoclonal antibodies?
Human cytokeratin fragment antigen 21-1 (CYFRA21-1) ELISA Kit

A:
Thanks for your inquiry.Pls check the details as below:
Capture:mouse monoclonal antibody;Detection:goat polyclonal antibody
If further questions,pls let me know.
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