Human death-associated protein kinase (DAPK) ELISA Kit

Instructions
Code CSB-E14181h
Size 96T,5×96T,10×96T

Product Details

Target Name death-associated protein kinase 1
Alternative Names DAPK, DKFZp781I035,
Abbreviation DAPK1
Uniprot No. P53355
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human DAPK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 85-94
1:2 Average % 87
Range % 82-94
1:4 Average % 93
Range % 87-98
1:8 Average % 104
Range % 100-110
Recovery
The recovery of human DAPK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 90-100
EDTA plasma (n=4) 97 92-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.609 2.534 2.572 2.487
1000 1.624 1.701 1.663 1.578
500 0.912 0.879 0.896 0.811
250 0.531 0.512 0.522 0.437
125 0.274 0.267 0.271 0.186
62.5 0.246 0.235 0.241 0.156
31.25 0.129 0.133 0.131 0.046
0 0.083 0.086 0.085  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.; FUNCTION
Subcellular Location Isoform 1: Cytoplasm, Cytoplasm, cytoskeleton
Protein Families Protein kinase superfamily, CAMK Ser/Thr protein kinase family, DAP kinase subfamily
Tissue Specificity Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.
Database Links

HGNC: 2674

OMIM: 600831

KEGG: hsa:1612

STRING: 9606.ENSP00000350785

UniGene: Hs.380277

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