Human dopamine-β-hydroxylase,DBH ELISA Kit

Instructions
Code CSB-E09653h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name dopamine beta-hydroxylase (dopamine beta-monooxygenase)
Alternative Names RP11-317B10.1, DBM, dopamine beta hydroxylase
Abbreviation DBH
Uniprot No. P09172
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 2.5 ng/mL-500 ng/mL
Sensitivity 1.25 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human DBH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 82-96  
1:2 Average % 95  
Range % 91-100  
1:4 Average % 98  
Range % 91-105  
1:8 Average % 93  
Range % 84-99  
Recovery
The recovery of human DBH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-99  
EDTA plasma (n=4) 97 90-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
0 1.958 1.963 1.961    
2.5 1.311 1.321 1.316    
10 0.812 0.836 0.824    
40 0.516 0.527 0.522    
125 0.319 0.324 0.322    
500 0.186 0.189 0.188    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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Target Data

Function Conversion of dopamine to noradrenaline.
Involvement in disease Dopamine beta-hydroxylase deficiency (DBH deficiency)
Subcellular Location Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen, Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen, Secreted, SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle membrane, Single-pass type II membrane protein, Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane, Single-pass type II membrane protein
Protein Families Copper type II ascorbate-dependent monooxygenase family
Database Links

HGNC: 2689

OMIM: 223360

KEGG: hsa:1621

STRING: 9606.ENSP00000376776

UniGene: Hs.591890

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