Human dynorphin,Dyn ELISA Kit

Code CSB-E09128h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation Dyn
Target Name dynorphin,Dyn
Alias N/A
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human Dyn in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 80-90  
1:2 Average % 95  
Range % 90-100  
1:4 Average % 93  
Range % 90-96  
1:8 Average % 92  
Range % 87-96  
The recovery of human Dyn spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 84-99  
EDTA plasma (n=4) 100 97-103  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 2.537 2.576 2.557 2.451  
100 2.141 2.164 2.153 2.047  
50 1.778 1.791 1.785 1.679  
25 1.405 1.455 1.430 1.324  
12.5 1.012 1.053 1.033 0.927  
6.25 0.604 0.624 0.614 0.508  
3.12 0.348 0.357 0.353 0.247  
0 0.105 0.107 0.106    
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