Rat dynorphin,Dyn ELISA Kit

Rat dynorphin,Dyn ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Rat dynorphin,Dyn ELISA Kit

Name: Rat dynorphin,Dyn ELISA Kit
Brand: CUSABIO
SKU: CSB-E13294r

Citations (4) Protocol MSDS

Code	CSB-E13294r
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (4)
Related Products
Customer Reviews and Q&A

Target Name

dynorphin,Dyn

Alternative Names

N/A

Abbreviation

Dyn

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

2.35 pg/mL-150 pg/mL

Sensitivity

0.58 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat Dyn in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

Recovery

The recovery of rat Dyn spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
150	2.135	2.169	2.152	2.027
75	1.766	1.798	1.782	1.657
37.5	1.364	1.330	1.347	1.222
18.7	0.853	0.846	0.850	0.725
9.4	0.454	0.461	0.458	0.333
4.7	0.303	0.323	0.313	0.188
2.35	0.217	0.231	0.224	0.099
0	0.124	0.126	0.125

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Rat Dyn ELISA Kit was designed for the quantitative measurement of Rat Dyn protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 2.35 pg/mL-150 pg/mL and the sensitivity is 0.58 pg/mL.

Acute Kappa opioid receptor blocking disrupts the pro-cognitive effect of cannabidiol in neuropathic rats S Boccella, A Fusco, F Ricciardi, AM Morace,Neuropharmacology,2024
Sex-dependent effect of inflammatory pain on negative affective states is prevented by kappa opioid receptors blockade in the nucleus accumbens shell JD Lorente,Neuropharmacology,2024
Sex-dependent effect of inflammatory pain on negative affective states is prevented by kappa opioid receptors blockade in the nucleus accumbens shell JD Lorente,biorxiv,2023
Controlled-release levodopa methyl ester/benserazide-loaded nanoparticles ameliorate levodopa-induced dyskinesia in rats /,Int J Nanomedicine,2012

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■ Q&A

Regarding your ELISA kit CSB-E13294r, could you please advise whether this kit is detecting Dynorphin A, Dynorphin A 1-8 or Dynorphin B, or if it detected all forms?

Thanks for your inquiry.
It detects Dynorphin B. Pls let me know if you have any further questions. Thank you.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.