Human endothelial cell-specific molecule 1(ESM-1) ELISA Kit

Instructions
Code CSB-E16530h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name endothelial cell-specific molecule 1
Alternative Names endocan,
Abbreviation ESM1
Uniprot No. Q9NQ30
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Tags & Cell Markers
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ESM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 81-92  
1:2 Average % 95  
Range % 91-105  
1:4 Average % 93  
Range % 90-100  
1:8 Average % 95  
Range % 85-98  
Recovery
The recovery of human ESM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 87 80-98  
EDTA plasma (n=4) 95 92-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.126 2.002 2.064 1.870  
5 1.973 1.876 1.925 1.731  
2.5 1.636 1.604 1.620 1.426  
1.25 1.186 1.139 1.163 0.969  
0.625 0.803 0.784 0.794 0.600  
0.312 0.574 0.539 0.557 0.363  
0.156 0.316 0.309 0.313 0.119  
0 0.199 0.188 0.194    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions.
Subcellular Location Secreted
Tissue Specificity Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney.
Database Links

HGNC: 3466

OMIM: 601521

KEGG: hsa:11082

STRING: 9606.ENSP00000370812

UniGene: Hs.129944

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