Human endothelial cell-specific molecule 1(ESM-1) ELISA Kit

Code CSB-E16530h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9NQ30
Lead Time 3-5 working days
Abbreviation ESM1
Target Name endothelial cell-specific molecule 1
Alias endocan,
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Tags & Cell Markers
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. Two transcript variants encoding different isoforms have been found for this gene.
HGNC 3466
RGD 71013
MGI 1918940
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ESM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 81-92  
1:2 Average % 95  
Range % 91-105  
1:4 Average % 93  
Range % 90-100  
1:8 Average % 95  
Range % 85-98  
Recovery
The recovery of human ESM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 87 80-98  
EDTA plasma (n=4) 95 92-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.126 2.002 2.064 1.870  
5 1.973 1.876 1.925 1.731  
2.5 1.636 1.604 1.620 1.426  
1.25 1.186 1.139 1.163 0.969  
0.625 0.803 0.784 0.794 0.600  
0.312 0.574 0.539 0.557 0.363  
0.156 0.316 0.309 0.313 0.119  
0 0.199 0.188 0.194    
References
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Function Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions.
Subcellular Location Secreted
Tissue Specificity Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney.
Database Links

HGNC: 3466

OMIM: 601521

KEGG: hsa:11082

STRING: 9606.ENSP00000370812

UniGene: Hs.129944

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