Human eosinophil cationic protein,ECP ELISA Kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

Name: Human eosinophil cationic protein,ECP ELISA Kit
Brand: CUSABIO
SKU: CSB-E11729h

Product Details

Target Name

eosinophil cationic protein,ECP

Alternative Names

Cytotoxic ribonuclease ELISA Kit; ECP ELISA Kit; ECP_HUMAN ELISA Kit; Eosinophil cationic protein ELISA Kit; OTTHUMP00000164017 ELISA Kit; Ribonuclease 3 ELISA Kit; Ribonuclease; RNase A family; 3 ELISA Kit; RNase 3 ELISA Kit; RNASE3 ELISA Kit; RNS3 ELISA Kit

Abbreviation

ECP

Uniprot No.

P12724

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

1.56 ng/mL-100 ng/mL

Sensitivity

0.39 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ECP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-98
1:2	Average %	97
	Range %	92-103
1:4	Average %	100
	Range %	94-108
1:8	Average %	108
	Range %	103-112

Recovery

The recovery of human ECP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-95
EDTA plasma (n=4)	92	89-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.500	2.493	2.497	2.392
50	2.003	1.998	2.001	1.896
25	1.275	1.266	1.271	1.166
12.5	0.587	0.573	0.580	0.475
6.25	0.369	0.357	0.363	0.258
3.12	0.221	0.208	0.215	0.110
1.56	0.176	0.169	0.173	0.068
0	0.108	0.102	0.105

Materials provided

A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ECP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled ECP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human ECP ELISA Kit was designed for the quantitative measurement of Human ECP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

Related Products

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

■ Q&A

I am interested in your Human eosinophil Cationic protein, ECP ELISA Kit (CSB-E11729h). However, I will test other markers and need advide for collection/preparation for this kit as stated below:
“I am hoping to get some information about the collection/preparation of serum prior to analysis. My lab will be analyzing serum samples for a variety of markers. the other markers all state to allow clotting for 30 minutes prior to centrifugation. The ECP (human) kit that I am interested in states to allow clotting for 2 hours. I was wondering if you could tell me if it needs to sit for two hours, or if 30 minutes if suffice. We are trying to avoid needed to process two separate samples so i'm hoping you can help me!”

Thanks for your inquiry.
CSB-E11729h
This kit can test serum and plasma samples. For serum samples, usually we will keep it static for 2 hours and then collect it. In this way more samples can be precipitated. What's more, if the static time is enough, the fibrin can timely trap hematocyte and then shrink and sink. Then if we treat it by centrifugation, the fibrin will not stay in the serum.
As it is mentioned, static for 30 minutes, we think that this static time is not very sufficient, and coagulation phenomenon is not very obvious. It is recommended to extend the static time. Later you should be able to see obvious coagulation and then extend the time. In this way more samples can be precipitated.
Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)

Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.

Gene References into Functions

measuring serum ECP levels is helpful for assessing the efficacy of inhaled corticosteroids therapy in bronchial asthma children. PMID: 28781336
Data suggest that radioactive I(131) therapy leads to down-regulation of secretion of ECP from Th2 cells (helper-inducer T-lymphocytes) in post-thyroidectomy patients treated for differentiated thyroid cancer; in this study, ECP levels in serum were analyzed before, 3 days after, and 7 days after radiotherapy with I(131). PMID: 29066479
our data add new insights into the pathophysiology of AD, suggesting that BDNF which correlates with disease severity and ECP levels contributes to the regulation of inflammation in an eosinophil-, but not Th2-dependent manner. PMID: 27087278
High circulating levels of eosinophil cationic protein are associated with increased incidence of ischemic stroke. PMID: 28904229
Studies mapped the structural determinants required for the eosinophil cationic protein (ECP)-bacterial lipopolysaccharide (LPS) interaction by nuclear magnetic resonance. PMID: 27696685
Increased MRP8/14 levels suggest the instability of the atherosclerotic plaque. ECP reflects the severity of coronary arteries stenosis, predicting atherosclerosis burden. They may become the new biomarkers of CAD. PMID: 27022611
Children in the acute-phase group showed higher ECP mRNA expression level than those in the stable-phase and healthy control groups, while the level in the stable-phase was markedly higher than that in the healthy control group. PMID: 26600485
drug eluting stent polymers increase eosinophil cationic protein levels following percutaneous coronary intervention independently of C-reactive protein PMID: 25463127
Around half of all variance in serum ECP is explained by genetic factors. Serum ECP is influenced by sex, BMI, and airway responsiveness. Serum ECP and airway responsiveness seem not to share genetic variance. PMID: 25354326
eosinophil cationic protein levels can be used to diagnosis atopic dermatitis but cannot distinguish between intrinsic and extrinsic disease. PMID: 24129707
The antimicrobial protein, Rnase 3, contributes to the host immune response against mycobacteria. PMID: 23716047
ECP was elevated in nasal discharge of patients with nasal polyps compared to controls and those with allergic rhinitis or rhinosinusitis. PMID: 23430080
report the prevalence of a common SNP in the eosinophil protein x/eosinophil-derived neurotoxin (EPX/EDN, RNase2) and the association with the cellular contents of EPX/EDN and ECP PMID: 24738159
Eosinophil cationic protein is not cytotoxic but enhances the growth of normal human dermal fibroblasts PMID: 24383969
Effects of omalizumab on eosinophil cationic peptide, 25-hydroxyvitamin-D, IL-1beta and sCD200 in cases of Samter's syndrome PMID: 23841472
Eosinophil activation occurred in early and late responses after L-ASA NPT in upper airway mucosa of AERD patients, where ApoA1, alpha2M and CP as well as CysLT may be involved in eosinophilic inflammation. PMID: 23786280
Eosinophil cationic protein is a major granule-derived protein with cytotoxic activity found in eosinophils and has been known as a useful marker of allergic inflammation. PMID: 23710948
Polymorphisms of EPX and ECP are associated to inflammatory bowel disease in an age and gender dependent manne. PMID: 23197886
novel insights to identify functional regions of hRNase3 that may have implications for all hRNaseA superfamily members. PMID: 23484086
This study also suggests that eosinophils and eosinophil cationic protein take part in the pathogenesis of nonallergic rhinitis. PMID: 23406588
Lower ECP levels in the active phase of the disease may be a result of decreased production due to the activation of Th1 cytokines. PMID: 23517396
A fraction of native ECP is stored in a non-cytotoxic form, which can be converted into a cytotoxic form by N-deglycosylation, whereas another fraction is stored as a highly cytotoxic form carrying different post-translational modifications PMID: 23597768
Eosinophil cationic protein might induce mesoderm differentiation through fibroblast growth factor signaling pathway and enhance subsequent cardiomyocyte differentiation in concert with dimethyl sulfoxide in P19CL6 cells. PMID: 22845717
a marker of asthma severity within an asthmatic paediatric population PMID: 21962901
Results reveal the structural basis for the high affinity of ECP for glycosaminoglycans. PMID: 22579681
biological and genetic characteristics of ECP PMID: 22845733
371G allele of RNASE3 is associated with susceptibility to cerebral malaria and forms part of a risk associated haplotype GGA defined by the markers PMID: 22216286
Total serum IgE, ECP and eosinophil percentage are strong predictors of allergic rhinitis. PMID: 22289536
The data suggests presence of relationship between development of restenosis and elevated blood plasma Eosinophilic cation protein level in patients with IHD after revascularization. PMID: 21878071
ECP heparin binding affinity depends on its RNase catalytic site PMID: 20213669
basal ECP levels are associated with major advemse cardiac evemts after bare metal stent implantation, suggesting that an allergic-mediated inflammation against the metal could explain some adverse reactions occurring after coronary stenting. PMID: 21183182
Arg22, Arg34, Arg61, Arg77 and His64 of ECP are crucial for eosinophil cationic proteins's membrane destabilization activity, which appears to be the underlying mechanism of its cytotoxic, antibacterial and antiparasitic activities. PMID: 21303303
The expression level of ECP mRNA was up-regulated in nasal polyps. PMID: 19086659
the shortest ECP minimized version that would retain its antimicrobial properties PMID: 20951760
ECP is present in the middle ear effusion of otitis media of effusion. There was a positive correlation between ECP level and IgE level. PMID: 16883790
Among mite-allergic patients T-bet expression was down-regulated, which had no relation to ECP concentration and allergic symptoms. PMID: 16874959
elevated levels in nasal lavage in allergic asthmatics PMID: 21038779
Eosinophil cationic protein may have a role in coronary atherosclerosis PMID: 20307883
There are associations of symptoms of allergy and asthma to ECP-genotypes, but also to smoking habits. ECP may be involved in impairment of lung functions in disease. PMID: 20534163
Serum levels of ECP, IL-5, -6, -8 and -10, G-CSF, MCP-1, IL-1 ra and IP-10 were significantly elevated in acute compared with stable childhhood asthma. PMID: 20523065
Results showed that OSCC patients with intense TATE and at least one C allele had a higher frequency of bilateral neck dissection, local recurrence, vascular embolization, involved resection margins, and postoperative radiotherapy. PMID: 19566743
Our study suggests that RNase3 polymorphisms are potentially associated with susceptibility to allergic rhinitis. PMID: 19760211
The eosinophil cationic protein increased TNF-alpha production in BEAS-2B cells and triggered apoptosis by caspase-8 activation through mitochondria-independent pathway. PMID: 20089176
The mean of serum ECP was significantly higher in patients with respiratory tract diseases (asthma, COPD, interstitial lung disease, and respiratory infections) compared with healthy volunteers. PMID: 20170318
Data show that conformational diversity of ECP is observed for the loop segments 17-22, 65-68, and 92-95 and most exposed sidechains. PMID: 19189375
Measurement of ECP polymorphism is a significant indicator for the disease activity in asthmatic patient. PMID: 12524255
serum levels elevated in asthmatic patients during acute attack PMID: 12530121
results suggest a differential release of ECP by the serum eosinophil, depending on the disease status and asthma exacerbation severity PMID: 12974194
Presence of ECP in human milk is associated with development of cow milk allergy and atopic dermatitis in the breast-fed infant, but has no direct association with the maternal atopy. PMID: 14630983
ECP contributes to surfactant dysfunction in asthma, which in turn could lead to airway obstruction. PMID: 15007353

Hide All

Subcellular Location

Secreted. Note=Located in the matrix of eosinophil large specific granule, which are released following activation by an immune stimulus.

Protein Families

Pancreatic ribonuclease family

Database Links

HGNC: 10046

OMIM: 131398

KEGG: hsa:6037

STRING: 9606.ENSP00000302324

UniGene: Hs.73839

Code	CSB-E11729h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.