Human eosinophil cationic protein,ECP ELISA Kit

Code CSB-E11729h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P12724
Lead Time 3-5 working days
Abbreviation ECP
Target Name eosinophil cationic protein,ECP
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ECP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %93
Range %88-98
1:2Average %97
Range %92-103
1:4Average %100
Range %94-108
1:8Average %108
Range %103-112
Recovery
The recovery of human ECP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9087-95
EDTA plasma (n=4)9289-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1002.500 2.493 2.497 2.392
502.003 1.998 2.001 1.896
251.275 1.266 1.271 1.166
12.50.587 0.573 0.580 0.475
6.250.369 0.357 0.363 0.258
3.120.221 0.208 0.215 0.110
1.560.176 0.169 0.173 0.068
00.108 0.102 0.105  
References
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Function Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
Subcellular Location Secreted
Protein Families Pancreatic ribonuclease family
Database Links

HGNC: 10046

OMIM: 131398

KEGG: hsa:6037

STRING: 9606.ENSP00000302324

UniGene: Hs.73839

Q&A and Customer Reviews

Q&A
Q:

I am interested in your Human eosinophil Cationic protein, ECP ELISA Kit (CSB-E11729h). However, I will test other markers and need advide for collection/preparation for this kit as stated below:
“I am hoping to get some information about the collection/preparation of serum prior to analysis. My lab will be analyzing serum samples for a variety of markers. the other markers all state to allow clotting for 30 minutes prior to centrifugation. The ECP (human) kit that I am interested in states to allow clotting for 2 hours. I was wondering if you could tell me if it needs to sit for two hours, or if 30 minutes if suffice. We are trying to avoid needed to process two separate samples so i'm hoping you can help me!”

A:
Thanks for your inquiry.
CSB-E11729h
This kit can test serum and plasma samples. For serum samples, usually we will keep it static for 2 hours and then collect it. In this way more samples can be precipitated. What's more, if the static time is enough, the fibrin can timely trap hematocyte and then shrink and sink. Then if we treat it by centrifugation, the fibrin will not stay in the serum.
As it is mentioned, static for 30 minutes, we think that this static time is not very sufficient, and coagulation phenomenon is not very obvious. It is recommended to extend the static time. Later you should be able to see obvious coagulation and then extend the time. In this way more samples can be precipitated.
Pls let me know if you have any further questions. Thank you.
Customer Reviews

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