Human galanin,GAL ELISA Kit

Code CSB-E09025h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P22466
Lead Time 3-5 working days
Abbreviation GAL
Target Name galanin prepropeptide
Alias GALN, GLNN, GMAP, MGC40167, galanin|galanin-message-associated peptide|galanin-related peptide
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 4.7 pg/mL-300 pg/mL
Sensitivity 1.17 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 917
RGD 1586615
MGI 1349461
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human GAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 81-89  
1:2 Average % 99  
Range % 95-103  
1:4 Average % 100  
Range % 98-102  
1:8 Average % 95  
Range % 90-98  
Recovery
The recovery of human GAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-97  
EDTA plasma (n=4) 95 90-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
300 2.618 2.556 2.587 2.478  
150 2.054 2.033 2.044 1.935  
75 1.237 1.212 1.225 1.116  
37.5 0.659 0.631 0.645 0.536  
18.75 0.413 0.398 0.406 0.297  
9.4 0.241 0.224 0.233 0.124  
4.7 0.175 0.163 0.169 0.060  
0 0.111 0.107 0.109    
References
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Function Endocrine hormone of the central and peripheral nervous systems that binds and activates the G protein-coupled receptors GALR1, GALR2, and GALR3. This small neuropeptide may regulate diverse physiologic functions including contraction of smooth muscle of the gastrointestinal and genitourinary tract, growth hormone and insulin release and adrenal secretion.
Involvement in disease Epilepsy, familial temporal lobe, 8 (ETL8)
Subcellular Location Secreted
Protein Families Galanin family
Database Links

HGNC: 4114

OMIM: 137035

KEGG: hsa:51083

STRING: 9606.ENSP00000265643

UniGene: Hs.278959

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