Human glycoprotein 130,gp130 ELISA KIT

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Alternative Names

CD130 ELISA Kit; CD130 antigen ELISA Kit; CDw130 ELISA Kit; gp130 ELISA Kit; GP130 RAPS ELISA Kit; IL 6R beta ELISA Kit; IL-6 receptor subunit beta ELISA Kit; IL-6R subunit beta ELISA Kit; IL-6R-beta ELISA Kit; IL-6RB ELISA Kit; IL6 ST ELISA Kit; IL6RB_HUMAN ELISA Kit; IL6ST ELISA Kit; Interleukin 6 receptor subunit beta ELISA Kit; Interleukin receptor beta chain ELISA Kit; Interleukin-6 receptor subunit beta ELISA Kit; Interleukin-6 signal transducer ELISA Kit; Membrane glycoprotein 130 ELISA Kit; Membrane glycoprotein gp130 ELISA Kit; Oncostatin M receptor ELISA Kit; Oncostatin M receptor alpha subunit ELISA Kit; Oncostatin-M receptor subunit alpha ELISA Kit

Abbreviation

gp130

Uniprot No.

P40189

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, saliva, urine.

Detection Range

0.24 ng/ml-15 ng/ml

Sensitivity

0.06 ng/ml

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human gp130 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:40	Average %	101
	Range %	94-108
1:80	Average %	93
	Range %	86-100
1:160	Average %	97
	Range %	92-103
1:320	Average %	90
	Range %	85-96

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.518

2.634

2.576

2.422

7.5

1.704

1.667

1.686

1.531

3.75

1.054

1.028

1.041

0.887

1.88

0.632

0.629

0.630

0.476

0.94

0.413

0.421

0.417

0.263

0.47

0.283

0.129

0.24

0.228

0.234

0.231

0.076

0.158

0.151

0.154

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This ELISA kit provides quantitative measurement of soluble gp130 (sgp130) in human samples, supporting research into cytokine signal transduction through the IL-6/JAK-STAT pathway. Because sgp130 functions as a natural inhibitor of IL-6 trans-signaling — a mechanism implicated in chronic inflammation and autoimmunity — precise quantification across both circulating and secreted fractions is essential for dissecting this regulatory axis.

Detection Range: The 0.24–15 ng/ml range aligns with reported circulating sgp130 concentrations in human serum and plasma, which typically fall between 100–400 ng/ml, meaning most serum samples will require dilution. Conditioned media from stimulated cells, where sgp130 shedding may be lower, can often be measured directly within this range.

Sensitivity: At 0.06 ng/ml, the kit detects sgp130 in low-abundance matrices such as saliva, urine, and conditioned media from unstimulated or primary cell cultures where shed gp130 levels are minimal.

Sample Compatibility: Validation across serum, plasma, cell culture supernatants, saliva, and urine enables researchers to correlate systemic sgp130 levels with local shedding at mucosal or renal sites, bridging in vivo and in vitro trans-signaling studies within a single assay platform.

Assay Efficiency: A 50–100 µl sample requirement and 1–5 hour protocol accommodate limited primary cell supernatants and high-throughput screening of patient cohorts.

This kit is directly applicable to studies of IL-6 trans-signaling in rheumatoid arthritis, inflammatory bowel disease, and cytokine storm pathophysiology.

Citations

Target Background

Function
(From Uniprot)

Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates the intracellular JAK-MAPK and JAK-STAT3 signaling pathways. That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3. In parallel, the IL6 signaling pathway induces the expression of two cytokine receptor signaling inhibitors, SOCS1 and SOCS3, which inhibit JAK and terminate the activity of the IL6 signaling pathway as a negative feedback loop. Also activates the yes-associated protein 1 (YAP) and NOTCH pathways to control inflammation-induced epithelial regeneration, independently of STAT3. Acts as a receptor for the neuroprotective peptide humanin as part of a complex with IL27RA/WSX1 and CNTFR. Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism. Has a role in embryonic development. Essential for survival of motor and sensory neurons and for differentiation of astrocytes. Required for expression of TRPA1 in nociceptive neurons. Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation. Required for normal trabecular bone mass and cortical bone composition.; Binds to the soluble IL6:sIL6R complex (hyper-IL6), thereby blocking IL6 trans-signaling. Inhibits sIL6R-dependent acute phase response. Also blocks IL11 cluster signaling through IL11R.

Gene References into Functions

Low soluble glycoprotein 130 (sgp130) level may serve as an additional indicator of coronary atherosclerosis severity. PMID: 29580104
we show by molecular replacement that Arg-112 does not participate in binding of IL-11 to its receptors IL-11R and glycoprotein 130 (gp130). Recombinant IL-11 R112H expressed in E. coli displays a correct four-helix-bundle folding topology, and binds with similar affinity to IL-11R and the IL-11/IL-11R/gp130 complex. PMID: 29237553
SNP rs10940495 in the gp130 locus was associated with functional outcome at 6 months following spontaneous intracerebral hemorrhage. PMID: 28964648
Low levels of serum sgp130 were positively associated with sex hormones in male patients with coronary artery disease, suggesting an important role of sgp130 in the presence of low and imbalanced sex hormones levels. PMID: 29028483
Decreased serum LIF levels may be associated with vasculopathy in systemic sclerosis (SSc) and that Fli1 deficiency may contribute to the inhibition of LIF-dependent biological effects on SSc endothelial cells by suppressing the expression of LIF, LIF receptor, and gp130. PMID: 29038846
gp130 is associated with adverse postoperative clinical outcomes of patients with late-stage non-metastatic gastric cancer. PMID: 27917904
GP130 signaling is required for tumor cell survival in diverse forms of ALK- anaplastic large cell lymphoma, even in the presence of JAK1/STAT3 mutations. PMID: 28356514
Data show that tumor suppressor adenomatous polyposis coli (APC) loss results in up-regulation of IL-6 signal transducer (IL-6ST/gp130), thereby activating YAP (YY1-associated) protein (YAP) which are simultaneously up-regulated in the majority of colorectal cancer (CRC). PMID: 28130546
an impaired oxidative stress response in hepatocytes with gp130 gain-of-function mutations, as detected in dysplastic intrahepatic nodules and hepatocellular adenomas, is one of the central oncogenic mechanisms in chronic liver inflammation. PMID: 27489351
age 3 plasma levels of sGP130 were found related to childhood asthma PMID: 27434124
This functional mouse model of PMM2-CDG, in vitro assays and identification of the novel gp130 biomarker all shed light on the human disease, and moreover, provide the essential tools to test potential therapeutics for this untreatable disease. PMID: 27053713
we developed a CRISPR-Cas9-based tool for specific DNA methylation consisting of deactivated Cas9 (dCas9) nuclease and catalytic domain of the DNA methyltransferase DNMT3A targeted by co-expression of a guide RNA to any 20 bp DNA sequence followed by the NGG trinucleotide.we demonstrated that directed DNA methylation of a wider promoter region of the target loci IL6ST and BACH2 decreased their expression PMID: 26969735
Intracellular p19 associated with the cytokine receptor subunit gp130 and stimulated the gp130-dependent activation of signal transducer and activator of transcription 3 (STAT3) signaling PMID: 26980441
High serum GP130 levels were associated with Chronic Spontaneous Urticaria. PMID: 26699882
Mutations leading to constitutive active gp130/JAK1/STAT3 pathway. PMID: 26188635
In the present study, we analyze the role of a unique cysteine residue (Cys-628) within this C-terminus, which is contained neither in the membrane-bound gp130 nor in the two other sgp130 forms. PMID: 26809098
pulmonary exposure to MWCNT can manifest as a reduced epithelial barrier and activator of vascular gp130-associated transsignaling that may promote susceptibility to cardiovascular derangements PMID: 26589480
Diabetic rats show an increase in the contractions of distal colon partly via the IL-6-IL-6 receptor pathway. PMID: 26191141
Data (including data from molecular dynamics simulation studies) suggest that conformation of GP130 (domains 2 and 3) changes and fluctuates only modestly (inter-domain angles vary only 125-140 degrees). PMID: 25243707
GP130 signaling collaborated with MYC to induce MM and was responsible and sufficient for directing the plasma cell phenotype. PMID: 25384216
GP130/STAT3 pathway is activated in NAFLD and can worsen insulin resistance while protecting against other lipotoxic mechanisms of disease pathogenesis. PMID: 25747354
NDRG2/gp130/STAT3 pathway can mediate the antimetastatic effects of Dp44mT in hepatocellular carcinoma. PMID: 25261367
Candidate gene IL6ST were elevated in male breast cancer biopsies compared to those from female patients without regard to Estrogen Receptors status. PMID: 24656773
Recombinant sgp130-E10Fc binds to a complex of IL-6 and sIL-6R, but not to IL-6 alone, and specifically inhibits IL-6 trans-signaling. Thus, it might play an important role in the regulation of trans-signaling in vivo. PMID: 24973212
Low incidence of IL6ST mutations in exon 6 are associated with lung cancer. PMID: 25242236
However, activation of the gp130 cell surface receptor is both necessary and sufficient for downregulation of TGF-beta2 in Kaposi's sarcoma-associated herpesvirus-infected cells. PMID: 25275137
This review showed that Humanin is a a possible linkage between Alzheimer's disease and type 2 diabetes. PMID: 24365186
Aberrant signalling from intracellular compartments might explain the tumorigenic potential of naturally occurring somatic mutations of gp130. PMID: 24213527
Data suggest women with inflammatory hepatic adenoma exhibit mutations in gp130/IL6ST (glycoprotein subunit 130, interleukin-6 receptor, HNF1A (hepatic nuclear factor 1 alpha), and CTNNB1 (beta-catenin). [REVIEW] PMID: 25076298
serum level decreased in early onset severe pre-eclampsia PMID: 23782174
ERK2 binds to the GP130 promoter, where it perhaps interacts with the transcriptional machinery. PMID: 23686311
Sgp130 serum levels are significantly higher in subjects carrying the gp130 polymorphism G148C compared to wildtype carriers. PMID: 24629561
Higher expression of p-p38, p-C-JUN, p-STAT3, and p-gp130 in the MBC-B group. PMID: 23813018
SC144 binds gp130, induces gp130 phosphorylation (S782) and deglycosylation, abrogates Stat3 phosphorylation and nuclear translocation, and further inhibits the expression of downstream target genes PMID: 23536726
Data indicate that CRLF1 serves its protective role by a cell autonomous mechanism that is independent of the gp130/JAK signaling pathway. PMID: 23818941
CNTF-mediated protection of photoreceptors requires initial activation of the cytokine receptor gp130 in Muller glial cells. PMID: 24191003
Newly synthesized IL-6 drives association of the IL-6 receptor and gp130 with EGFR, leading to EGFR-dependent rephosphorylation of STAT3, which is not inhibited by the continued presence of SOCS3. PMID: 24082147
Data suggest that soluble gp130 has a role in prostate cancer invasion in an interleukin-6 dependent and independent manner. PMID: 21944124
Data suggest flavanoids (naringenin, flavone) suppress interleukin-6/IL6ST signaling and induce expression of SOCS3 (signal transducer and activator of transcription 3) in vascular endothelium; thus, dietary factors act as anti-inflammatory agents. PMID: 23782265
gene expression and surface expression in NK92 cells of ciliary neurotrophic factor alpha (CNTFR-alpha) and glycoprotein (gp)130 in natural killer (NK) cells, in the context of malaria, were investigated. PMID: 23398366
interleukin-6 receptor polymorphism may be a contributing factor in Castleman Disease, and further research is warranted. PMID: 23372742
In a laboratory study, addition of gp130 to culture media improves blastocyst formation and implicates a physiological role in regulating preimplantation development in humans. PMID: 23375201
Data indicate that active cruzipain completely abrogated the STAT3 phosphorylation and enzymatically cleaved gp130 ectodomain. PMID: 23253440
An interleukin-6 receptor-dependent molecular switch mediates signal transduction of the IL-27 cytokine subunit p28 (IL-30) via a gp130 protein receptor homodimer. PMID: 23209286
gp130 activation is regulated by D2-D3 interdomain connectivity. PMID: 23294003
Gp130 mRNA was hardly detectable in endometrium of both fertile and infertile women with no difference between them. Infertile women secreted significantly less gp130 molecules in the uterine flushing compared with fertile women. PMID: 22520363
Together these results suggest that the inhibitory effect of OSM on TGF-beta1-induced CTGF mRNA expression is mainly driven by STAT3, thereby providing a signaling mechanism whereby OSM may contribute to tubulointerstitial protection. PMID: 22814105
the mutant gp130 receptor protein from inflammatory hepatocellular adenoma is inhibited by an anti-gp130 antibody that specifically neutralizes interleukin 11 signaling PMID: 22523320
gp130 regulates interleukin 6 signalling as an antagonist in cerebrospinal fluid immediately after subarachnoid haemorrhage PMID: 20660911
The IL-6/gp130 signaling pathway is dierctly involved in amyloid-beta precursor protein (APP)-induced glial differentiation. PMID: 20309664

Hide All

Subcellular Location

[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.

Protein Families

Type I cytokine receptor family, Type 2 subfamily

Tissue Specificity

Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.; [Isoform 2]: Expressed in blood serum (at protein level).

Database Links

HGNC: 6021

OMIM: 600694

KEGG: hsa:3572

STRING: 9606.ENSP00000338799

UniGene: Hs.532082

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-E04571h-IS
Size	96T,5×96T,10×96T
Price	Request a Quote
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
Have Questions?	Leave a Message or Start an on-line Chat

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.