Human glypican 6 (GPC6) ELISA kit

Code CSB-EL009708HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
glypican 6
Alternative Names
A Kit; Glypican 6 [Precursor] ELISA Kit; Glypican proteoglycan 6 ELISA Kit; Gpc6 ELISA Kit; GPC6_HUMAN ELISA Kit; MGC126288 ELISA Kit; OMIMD1 ELISA Kit; PRO705 ELISA Kit; Secreted glypican 6 ELISA Kit; Secreted glypican-6 ELISA Kit; UNQ369 ELISA Kit
Abbreviation
GPC6
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human GPC6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 87    
Range % 82-91    
1:2 Average % 94    
Range % 89-98    
1:4 Average % 107    
Range % 103-110    
1:8 Average % 110    
Range % 104-114    
Recovery
The recovery of human GPC6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 95 91-99    
EDTA plasma (n=4) 103 98-108    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
20 2.443 2.403 2.423 2.271    
10 1.692 1.702 1.697 1.545    
5 1.218 1.268 1.243 1.091    
2.5 0.854 0.865 0.860 0.708    
1.25 0.602 0.612 0.607 0.455    
0.625 0.413 0.403 0.408 0.256    
0.312 0.274 0.268 0.271 0.119    
0 0.153 0.150 0.152      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human GPC6 ELISA Kit was designed for the quantitative measurement of Human GPC6 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Citations

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Target Background

Function
(From Uniprot)
Cell surface proteoglycan that bears heparan sulfate. Putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. Enhances migration and invasion of cancer cells through WNT5A signaling.
Gene References into Functions
  1. Thus, GPC6 and TMEM132D may serve as predictors of CD8+ T-lymphocyte infiltration and as favorable prognostic markers in early stage ovarian cancer with important consequences for diagnosis. PMID: 26448945
  2. Extracellular matrix proteins expression profiling in chemoresistant variants of the A2780 ovarian cancer cell line. PMID: 24804215
  3. GPC6 promotes invasive migration by inhibition of canonical beta-catenin and Wnt signalling, and up-regulation of non-canonical Wnt5A signalling leading to the activation of JNK and p38 MAPK. PMID: 21871017
  4. a reduction in GPC6 mRNA in retinoblastoma is associated with the non-random allelic loss at 13q31 that could contribute to RB development. PMID: 19726429
  5. Mutations in the heparan-sulfate proteoglycan glypican 6 impair endochondral ossification and cause recessive omodysplasia PMID: 19481194

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Involvement in disease
Omodysplasia 1 (OMOD1)
Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.; [Secreted glypican-6]: Secreted, extracellular space.
Protein Families
Glypican family
Tissue Specificity
Widely expressed. High expression in fetal kidney and lung and lower expressions in fetal liver and brain. In adult tissues, very abundant in ovary, high levels also observed in liver, kidney, small intestine and colon. Not detected in peripheral blood le
Database Links

HGNC: 4454

OMIM: 258315

KEGG: hsa:10082

STRING: 9606.ENSP00000366246

UniGene: Hs.444329

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