Human glypican 6 (GPC6) ELISA kit

Code CSB-EL009708HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9Y625
Lead Time 7-14 working days
Abbreviation GPC6
Target Name glypican 6
Alias MGC126288, OMIMD1, glypican proteoglycan 6
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details The glypicans comprise a family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycans, and they have been implicated in the control of cell growth and cell division. The glypican encoded by this gene is a putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases.
HGNC 4454
MGI 1346322
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human GPC6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 87    
Range % 82-91    
1:2 Average % 94    
Range % 89-98    
1:4 Average % 107    
Range % 103-110    
1:8 Average % 110    
Range % 104-114    
Recovery
The recovery of human GPC6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 95 91-99    
EDTA plasma (n=4) 103 98-108    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
20 2.443 2.403 2.423 2.271    
10 1.692 1.702 1.697 1.545    
5 1.218 1.268 1.243 1.091    
2.5 0.854 0.865 0.860 0.708    
1.25 0.602 0.612 0.607 0.455    
0.625 0.413 0.403 0.408 0.256    
0.312 0.274 0.268 0.271 0.119    
0 0.153 0.150 0.152      
References
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Function Cell surface proteoglycan that bears heparan sulfate. Putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases (By similarity). Enhances migration and invasion of cancer cells through WNT5A signaling.
Involvement in disease Omodysplasia 1 (OMOD1)
Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor, Extracellular side, SUBCELLULAR LOCATION: Secreted glypican-6: Secreted, extracellular space
Protein Families Glypican family
Tissue Specificity Widely expressed. High expression in fetal kidney and lung and lower expressions in fetal liver and brain. In adult tissues, very abundant in ovary, high levels also observed in liver, kidney, small intestine and colon. Not detected in peripheral blood le
Database Links

HGNC: 4454

OMIM: 258315

KEGG: hsa:10082

STRING: 9606.ENSP00000366246

UniGene: Hs.444329

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