Human guanylate cyclase activator 2B (GUCA2B) ELISA kit

Code CSB-EL010048HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
guanylate cyclase activator 2B
Alternative Names
GUCA2B ELISA Kit; GCAP II ELISA Kit; GCAP-II ELISA Kit; Guanylate cyclase activator 2B (uroguanylin) ELISA Kit; Guanylate cyclase activator 2B ELISA Kit; Guanylate cyclase C-activating peptide II ELISA Kit; GUC2B_HUMAN ELISA Kit; GUCA2B ELISA Kit; UGN ELISA Kit; Uroguanylin ELISA Kit
Abbreviation
GUCA2B
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, urine, cell culture supernates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human GUCA2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 89  
Range % 85-91  
1:10 Average % 102  
Range % 98-105  
1:20 Average % 99  
Range % 93-105  
1:40 Average % 95  
Range % 92-97  
Recovery
The recovery of human GUCA2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 91-96  
EDTA plasma (n=4) 92 87-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.155 2.175 2.165 2.038  
1000 1.640 1.633 1.637 1.510  
500 1.069 1.054 1.062 0.935  
250 0.680 0.689 0.685 0.558  
125 0.438 0.424 0.431 0.304  
62.5 0.266 0.287 0.277 0.150  
31.25 0.185 0.176 0.181 0.054  
0 0.129 0.125 0.127    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human GUCA2B ELISA Kit was designed for the quantitative measurement of Human GUCA2B protein in serum, plasma, urine, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL .

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins. May be a potent physiological regulator of intestinal fluid and electrolyte transport. May be an autocrine/paracrine regulator of intestinal salt and water transport.
Gene References into Functions
  1. The simulations suggested that all missense SNPs considered as convergent deleterious caused some kind of structural change to the uroguanylin peptide. Additionally, four of these SNPs were also shown to cause modifications in peptide flexibility, possibly resulting in functional changes. PMID: 27620667
  2. Uroguanylin concentrations are increased in patients with chronic renal failure, nephrotic syndrome, or those on dialysis. PMID: 15780094
  3. The occurrences of the C-A haplotype (rs883062-rs1047047) and the C-A-G haplotype (rs883062-rs1047047-rs2297566) were significantly higher in the essential hypertension group than in the controls. PMID: 18037771
Subcellular Location
Secreted.
Protein Families
Guanylin family
Tissue Specificity
Stomach and intestine.
Database Links

HGNC: 4683

OMIM: 601271

KEGG: hsa:2981

STRING: 9606.ENSP00000361662

UniGene: Hs.32966

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1