Human hepatocyte growth factor activator(HGFA) ELISA kit

Code CSB-E13160h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q04756
Lead Time 3-5 working days
Abbreviation HGFAC
Target Name HGF activator
Alias RP11-529E10.2, HGFA, MGC138395, MGC138397,
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein belongs to peptidase family S1. It is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form.
HGNC 4894
RGD 70909
MGI 1859281
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HGFA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %84
Range %81-89
1:2Average %95
Range %91-99
1:4Average %87
Range %82-93
1:8Average %105
Range %101-109
Recovery
The recovery of human HGFA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8985-91
EDTA plasma (n=4)9491-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
50002.117 2.148 2.133 2.025
25001.696 1.682 1.689 1.581
12501.215 1.268 1.242 1.134
6250.834 0.816 0.825 0.717
3120.502 0.536 0.519 0.411
1560.265 0.258 0.262 0.154
780.168 0.171 0.170 0.062
00.106 0.109 0.108
References
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Function Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.
Subcellular Location Secreted
Protein Families Peptidase S1 family
Tissue Specificity Liver.
Database Links

HGNC: 4894

OMIM: 604552

KEGG: hsa:3083

STRING: 9606.ENSP00000372224

UniGene: Hs.104

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