Human hepatocyte growth factor activator(HGFA) ELISA kit

Instructions
Code CSB-E13160h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name HGF activator
Alternative Names RP11-529E10.2, HGFA, MGC138395, MGC138397,
Abbreviation HGFAC
Uniprot No. Q04756
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HGFA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %84
Range %81-89
1:2Average %95
Range %91-99
1:4Average %87
Range %82-93
1:8Average %105
Range %101-109
Recovery
The recovery of human HGFA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8985-91
EDTA plasma (n=4)9491-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
50002.117 2.148 2.133 2.025
25001.696 1.682 1.689 1.581
12501.215 1.268 1.242 1.134
6250.834 0.816 0.825 0.717
3120.502 0.536 0.519 0.411
1560.265 0.258 0.262 0.154
780.168 0.171 0.170 0.062
00.106 0.109 0.108
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

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Target Data

Function Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.
Subcellular Location Secreted
Protein Families Peptidase S1 family
Tissue Specificity Liver.
Database Links

HGNC: 4894

OMIM: 604552

KEGG: hsa:3083

STRING: 9606.ENSP00000372224

UniGene: Hs.104

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