Code | CSB-E04590h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human IGFBP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:10 | Average % | 92 | ||||
Range % | 82-103 | |||||
1:20 | Average % | 95 | ||||
Range % | 91-99 | |||||
1:40 | Average % | 95 | ||||
Range % | 90-100 | |||||
1:80 | Average % | 91 | ||||
Range % | 85-100 |
The recovery of human IGFBP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 98 | 92-106 | ||||
EDTA plasma (n=4) | 95 | 90-100 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The product CSB-E04590h is a sandwich ELISA kit developed to measure concentrations of human IGFBP3 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of IGFBP3 in samples. The color intensity is measured at 450 nm via a microplate reader.
IGFBP3 can regulate the mitogenic activity of insulin-like growth factor 1 receptor (IGF1R). IGF-independent roles of IGFBP3 include its interactions with the extracellular, membrane, and intracellular proteins and translocation into the cytoplasm and into the nucleus. Aberrant expression or malfunction of IGFBP3 is related to cancer development and progression. IGFBP3 functions as either an oncogene or tumor suppressor depending on the cellular context and tumor type. A series of studies have shown that IGFBP3 inhibits cell adhesion, invasiveness of endometrial cancer, metastasis in prostate cancer, and angiogenesis in head and neck squamous cell carcinoma. On the contrary, IGFBP3 has antioxidative activity, inhibiting ROS, and enhancing epithelial-to-mesenchymal transition and motility, which is required for tumor progression.
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