Human insulin-like growth factors binding protein 3,IGFBP-3 ELISA Kit

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Target Background

Name: Human insulin-like growth factors binding protein 3,IGFBP-3 ELISA Kit
Brand: CUSABIO
SKU: CSB-E04590h

Product Details

Target Name

insulin-like growth factor binding protein 3

Alternative Names

Acid stable subunit of the 140 K IGF complex ELISA Kit; Binding protein 29 ELISA Kit; Binding protein 53 ELISA Kit; BP 53 ELISA Kit; BP53 ELISA Kit; Growth hormone dependent binding protein ELISA Kit; IBP 3 ELISA Kit; IBP-3 ELISA Kit; IBP3 ELISA Kit; IBP3_HUMAN ELISA Kit; IGF binding protein 3 ELISA Kit; IGF-binding protein 3 ELISA Kit; IGFBP 3 ELISA Kit; IGFBP-3 ELISA Kit; IGFBP3 ELISA Kit; Insulin Like Growth Factor Binding Protein 3 ELISA Kit; Insulin-like growth factor binding protein 3 precursor ELISA Kit; Insulin-like growth factor-binding protein 3 ELISA Kit

Abbreviation

IGFBP3

Uniprot No.

P17936

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.78 ng/mL-50 ng/mL

Sensitivity

0.195 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IGFBP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	92
	Range %	82-103
1:20	Average %	95
	Range %	91-99
1:40	Average %	95
	Range %	90-100
1:80	Average %	91
	Range %	85-100

Recovery

The recovery of human IGFBP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-106
EDTA plasma (n=4)	95	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.119	2.119	2.119	1.957
25	1.560	1.521	1.541	1.379
12.5	1.012	1.080	1.046	0.884
6.25	0.716	0.726	0.721	0.559
3.12	0.471	0.454	0.463	0.301
1.56	0.321	0.334	0.328	0.166
0.78	0.235	0.243	0.239	0.077
0	0.161	0.163	0.162

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The product CSB-E04590h is a sandwich ELISA kit developed to measure concentrations of human IGFBP3 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of IGFBP3 in samples. The color intensity is measured at 450 nm via a microplate reader.

IGFBP3 can regulate the mitogenic activity of insulin-like growth factor 1 receptor (IGF1R). IGF-independent roles of IGFBP3 include its interactions with the extracellular, membrane, and intracellular proteins and translocation into the cytoplasm and into the nucleus. Aberrant expression or malfunction of IGFBP3 is related to cancer development and progression. IGFBP3 functions as either an oncogene or tumor suppressor depending on the cellular context and tumor type. A series of studies have shown that IGFBP3 inhibits cell adhesion, invasiveness of endometrial cancer, metastasis in prostate cancer, and angiogenesis in head and neck squamous cell carcinoma. On the contrary, IGFBP3 has antioxidative activity, inhibiting ROS, and enhancing epithelial-to-mesenchymal transition and motility, which is required for tumor progression.

Code	CSB-E04590h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.